Difference between revisions of "Part:BBa K1055000"
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''Characterization mKate'' | ''Characterization mKate'' | ||
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[[Image:FRET_Normalized_2.png|400px|center|thumb|Figure 3. '''Overlay of excitation spectrum (dashed line) and emission spectrum (solid line) of mKate and LSSmOrange ''']] | [[Image:FRET_Normalized_2.png|400px|center|thumb|Figure 3. '''Overlay of excitation spectrum (dashed line) and emission spectrum (solid line) of mKate and LSSmOrange ''']] | ||
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+ | Moreover, unlike for our LssmOrange BioBrick (BBa_K1055001), there is a crystal structure available for further analysis which is shown below: | ||
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+ | [[Image:mkate-active_surface2_light1.png|400px|thumb|center|Figure 2. '''Excitation spectrum (dashed line) and emission spectrum (solid line) of mKate with marked maximums''' ]] | ||
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Revision as of 17:54, 12 October 2013
mKate is a fluorophor derived from dsRed
mKate is a fluorophor derived from dsRed and is a FRET partner of LssmOrange [BBa_K1055001]
Usage and Biology
Characterization mKate
mKate is a red fluorescent protein that we want to use as a acceptor for FRET (Figure 3). According to [http://www.evrogen.com/products/basicFPs.shtml Evrogen] mKate has an excitation maximum at 588 nm and an emission maximum at 633 nm. Figure 2 shows that the excitation maximum is at 579 nm and the emission maximum is 611 nm. The emission maximum is big enough to get stimulated by LSSmOrange, the other fluorescent protein. Of course, we checked also the emission and excitation of our BL21(DE3) cells (data not shown) and we can eliminate the theories that these cells disturb this florescence measurement.
Moreover, unlike for our LssmOrange BioBrick (BBa_K1055001), there is a crystal structure available for further analysis which is shown below:
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]