Difference between revisions of "Part:BBa K1071009"
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the fluorescent protein eGFP, codon usage of'' E. coli'', works also in ''Phaeodactylum tricornutum''. The excitation peak is at 488nm and the emission at 507nm.
 
the fluorescent protein eGFP, codon usage of'' E. coli'', works also in ''Phaeodactylum tricornutum''. The excitation peak is at 488nm and the emission at 507nm.
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biology of this part here
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<!-- Add more about the biology of this part here
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===Usage and Biology===
 
===Usage and Biology===
  
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In the following picture we demostrate that both promotors (i.e. <html><a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K1071004">PNR</a></html> and <html><a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K1071003">PfcpB</a></html>) work properly as shown by the expression of eGFP (green, second panel). The red color (third panel) is due to autoflourescence of the plastides and indicates their localisation within P. tricornutum. The very left and the very right panel represent DIC and an overlay of green and red, respectively.
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<html><img src="https://static.igem.org/mediawiki/2013/1/12/416_496.jpg" alt="eGFP in P. tricornutum" /></html>
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eGFP localisation in ''P. tricornutum''.
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K1071009 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1071009 SequenceAndFeatures</partinfo>

Revision as of 17:54, 12 October 2013

eGFP

the fluorescent protein eGFP, codon usage of E. coli, works also in Phaeodactylum tricornutum. The excitation peak is at 488nm and the emission at 507nm. 

biology of this part here

Usage and Biology

In the following picture we demostrate that both promotors (i.e. PNR and PfcpB) work properly as shown by the expression of eGFP (green, second panel). The red color (third panel) is due to autoflourescence of the plastides and indicates their localisation within P. tricornutum. The very left and the very right panel represent DIC and an overlay of green and red, respectively.

eGFP in P. tricornutum eGFP localisation in P. tricornutum.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]