Difference between revisions of "Part:BBa K1055001"
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===Applications of BBa_K1055001===
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===Usage and Biology===
  
 
[[Image:LSSmOrange_bac.png|400px|right|thumb|Figure 1. '''Pellet of ''E. coli'' BL21 (DE3) cells with expressed LSSmOrange. Left side without UV radiation, right side with UV radiation''']]
 
[[Image:LSSmOrange_bac.png|400px|right|thumb|Figure 1. '''Pellet of ''E. coli'' BL21 (DE3) cells with expressed LSSmOrange. Left side without UV radiation, right side with UV radiation''']]
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[[Image:LSSmOrange_Diagramm.png|left|400px|thumb|Figure 2. '''Excitation spectrum (dashed line) and emission spectrum (solid line) of LSSmOrange with marked maximums''']]
 
[[Image:LSSmOrange_Diagramm.png|left|400px|thumb|Figure 2. '''Excitation spectrum (dashed line) and emission spectrum (solid line) of LSSmOrange with marked maximums''']]
 
[[Image:FRET_Normalized_2.png|left|400px|thumb|Figure 3. '''Overlay of exitation spectrum (dashed line) and emission (solid line) of mKate and LSSmOrange''']]
 
[[Image:FRET_Normalized_2.png|left|400px|thumb|Figure 3. '''Overlay of exitation spectrum (dashed line) and emission (solid line) of mKate and LSSmOrange''']]
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===Usage and Biology===
 
  
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Furthermore we did homology modeling with this part, please refer to our [[http://2013.igem.org/Team:TU_Darmstadt/modelling/Structure wiki page]], whose best model results are illustrated below:
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[[Image:LssmOrange_first_activeHelix.png|left|400px|thumb|Figure 3. '''Overlay of exitation spectrum (dashed line) and emission (solid line) of mKate and LSSmOrange''']]
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Revision as of 17:49, 12 October 2013

LssmOrange is a fluorophor derived from dsRed

LssmOrange is a fluorophor derived from dsRed and is a FRET partner of mKate [BBa_K1055000]


Usage and Biology

Figure 1. Pellet of E. coli BL21 (DE3) cells with expressed LSSmOrange. Left side without UV radiation, right side with UV radiation

LSSmOrange is an orange fluorescent protein that we want to use for FRET, we us it as a donor. LSSmOrange has an excitation maximum by 437 nm and an emission maximum by 572 nm. The excitation maximum is at 415 nm, another at 453 nm and our measured emission maximum is at 564 nm (Fig. 2). This aberration changes nothing on the FRET system. The excitation maximum is big enough to stimulate mKate, the other fluorescent protein (Fig. 3). Of course, we also checked the emission and excitation of our E. coli BL21(DE3) cells (data not shown) and we can eliminate the theories that these cells disturb this florescence measurement.

Figure 2. Excitation spectrum (dashed line) and emission spectrum (solid line) of LSSmOrange with marked maximums
Figure 3. Overlay of exitation spectrum (dashed line) and emission (solid line) of mKate and LSSmOrange

Furthermore we did homology modeling with this part, please refer to our http://2013.igem.org/Team:TU_Darmstadt/modelling/Structure wiki page, whose best model results are illustrated below:

Figure 3. Overlay of exitation spectrum (dashed line) and emission (solid line) of mKate and LSSmOrange


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 570
  • 1000
    COMPATIBLE WITH RFC[1000]