Difference between revisions of "Part:BBa K1045011:Experience"
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===Applications of BBa_K1045011=== | ===Applications of BBa_K1045011=== | ||
| − | We used this part in our DarR reporter system [[Part:BBa_K1045017|BBa_K1045017]]. [[Part:BBa_K1045011|BBa_K1045011]] was functional as our characterization experiments of [[Part:BBa_K1045017|BBa_K1045017]] suggested. In this system, ''gfp'' is placed under the control of the DarR operator [[Part:BBa_K1045000|BBa_K1045000]]. We saw that GFP expression was repressed when ''E. coli'' harbored a construct carrying DarR under the control of the promoter in [[Part:BBa_K1045011|BBa_K1045011]]. For more information, see main page of [[Part:BBa_K1045017|BBa_K1045017]] and our entry on the experience page of BBa_K1045017: [ | + | We used this part in our DarR reporter system [[Part:BBa_K1045017|BBa_K1045017]]. [[Part:BBa_K1045011|BBa_K1045011]] was functional as our characterization experiments of [[Part:BBa_K1045017|BBa_K1045017]] suggested. In this system, ''gfp'' is placed under the control of the DarR operator [[Part:BBa_K1045000|BBa_K1045000]]. We saw that GFP expression was repressed when ''E. coli'' harbored a construct carrying DarR under the control of the promoter in [[Part:BBa_K1045011|BBa_K1045011]]. For more information, see main page of [[Part:BBa_K1045017|BBa_K1045017]] and our entry on the experience page of BBa_K1045017: |
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| + | [[File:-DarR.jpg|420px|thumb|'''Fig. 1.''': ''Top'': ''E. coli'' transformed with a plasmid encoding [[Part:BBa_K1045013|BBa_K1045013]] shows a strong green fluorescence under the fluorescence microscope. ''Bottom'': ''E. coli'' transformed with a plasmid harboring the DarR reporter system barely shows fluorescence. [[File:+DarR.jpg|420px]]|center]] | ||
===User Reviews=== | ===User Reviews=== | ||
Revision as of 17:36, 12 October 2013
This experience page is provided so that any user may enter their experience using this part.
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how you used this part and how it worked out.
Applications of BBa_K1045011
We used this part in our DarR reporter system BBa_K1045017. BBa_K1045011 was functional as our characterization experiments of BBa_K1045017 suggested. In this system, gfp is placed under the control of the DarR operator BBa_K1045000. We saw that GFP expression was repressed when E. coli harbored a construct carrying DarR under the control of the promoter in BBa_K1045011. For more information, see main page of BBa_K1045017 and our entry on the experience page of BBa_K1045017:
Fig. 1.: Top: E. coli transformed with a plasmid encoding BBa_K1045013 shows a strong green fluorescence under the fluorescence microscope. Bottom: E. coli transformed with a plasmid harboring the DarR reporter system barely shows fluorescence. 
User Reviews
UNIQ0af2eabdc32cd53c-partinfo-00000000-QINU UNIQ0af2eabdc32cd53c-partinfo-00000001-QINU