Difference between revisions of "Part:BBa K1223005"

 
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<partinfo>BBa_K1223005 short</partinfo>
 
<partinfo>BBa_K1223005 short</partinfo>
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===Usage and Biology===
 
===Usage and Biology===
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===Design notes===
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This part was designed to function as a biological timer for our P.A.S.E 1 system. It includes an assembly of two existing parts from the Registry, Lac/Ara-1 IPTG Inducible promoter (BBa_K354000) and LVA-tagged cI repressor protein (BBa_K327018). In order to extend the half life of the cI protein, we removed the LVA tail.
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The part was characterized through sequencing and restriction digest with PvuII and HindIII.
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[[File:cI1.png]]
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lane1: undigested pUC57-cI
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lane2: pUC57-cI digested with PvuII and HindIII
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lane3: DS5000 ladder
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In addition, the promoter's performance under the presence of IPTG and Arabinose was analyzed via comassie staining. The cI protein is 29 KDa.
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[[File:cI2.jpg]]
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===Source===
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genebank, iGEM parts registry
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===References===
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Revision as of 12:41, 12 October 2013

cI translational unit

Lambda cI translational unit with BBa_K345000 as promoter

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]