Difference between revisions of "Part:BBa K1202109:Experience"
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| − | Inducible promoter experiment | + | ===Inducible promoter experiment=== |
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To observe inducible promoters response, we co incubated two different bacteria. The first bacteria culture is expressing enzyme system and the second culture is including inducible promoter. At the experiment, we incubated them seperately for 16 hours and mixed them into one flask and 4 hour later, we add 0,1 mM SAH (with 10x PBS containing %1 BSA). Incubate with SAH for one day and, took 2 ml of liquid culture to santrifuge tube.Centrifuged it. We saw the red color at the pellet as you see below. | To observe inducible promoters response, we co incubated two different bacteria. The first bacteria culture is expressing enzyme system and the second culture is including inducible promoter. At the experiment, we incubated them seperately for 16 hours and mixed them into one flask and 4 hour later, we add 0,1 mM SAH (with 10x PBS containing %1 BSA). Incubate with SAH for one day and, took 2 ml of liquid culture to santrifuge tube.Centrifuged it. We saw the red color at the pellet as you see below. | ||
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| + | [[File:Co-incubation.png]] | ||
So, it means the enzyme system is working and inducible promoter is succesfully induced from AI-2. | So, it means the enzyme system is working and inducible promoter is succesfully induced from AI-2. | ||
| − | Discussion | + | '''Discussion''' |
We were searching for inducible system that, getting activated with cancer cell antigen precense. As an activating molecule, autoinducer2 activates LsrR-LsrK promoter system for express the killer protein by bacteria. | We were searching for inducible system that, getting activated with cancer cell antigen precense. As an activating molecule, autoinducer2 activates LsrR-LsrK promoter system for express the killer protein by bacteria. | ||
Latest revision as of 10:32, 9 October 2013
Inducible promoter experiment
To observe inducible promoters response, we co incubated two different bacteria. The first bacteria culture is expressing enzyme system and the second culture is including inducible promoter. At the experiment, we incubated them seperately for 16 hours and mixed them into one flask and 4 hour later, we add 0,1 mM SAH (with 10x PBS containing %1 BSA). Incubate with SAH for one day and, took 2 ml of liquid culture to santrifuge tube.Centrifuged it. We saw the red color at the pellet as you see below.
So, it means the enzyme system is working and inducible promoter is succesfully induced from AI-2.
Discussion
We were searching for inducible system that, getting activated with cancer cell antigen precense. As an activating molecule, autoinducer2 activates LsrR-LsrK promoter system for express the killer protein by bacteria.
Our co-incubation liquid cultures’ santrifuged tubes are seemin red colored.We added rfp gene to continue of inducible promotor gene and rfp is marker of activating. So this co incubation result significantly shows us promoter system is activating in the precense of autoindcer-2.
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