Difference between revisions of "Part:BBa K1159315"
m |
m |
||
| Line 10: | Line 10: | ||
<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
<partinfo>BBa_K1159315 SequenceAndFeatures</partinfo> | <partinfo>BBa_K1159315 SequenceAndFeatures</partinfo> | ||
| − | + | <html><!--- Please copy this table containing parameters for BBa_ at the end of the parametrs section ahead of the references. ---><style type="text/css">table#AutoAnnotator {border:1px solid black; width:100%; border-collapse:collapse;} th#AutoAnnotatorHeader { border:1px solid black; width:100%; background-color: rgb(221, 221, 221);} td.AutoAnnotator1col { width:100%; border:1px solid black; } span.AutoAnnotatorSequence { font-family:'Courier New', Arial; } td.AutoAnnotatorSeqNum { text-align:right; width:2%; } td.AutoAnnotatorSeqSeq { width:98% } td.AutoAnnotatorSeqFeat1 { width:3% } td.AutoAnnotatorSeqFeat2a { width:27% } td.AutoAnnotatorSeqFeat2b { width:97% } td.AutoAnnotatorSeqFeat3 { width:70% } table.AutoAnnotatorNoBorder { border:0px; width:100%; border-collapse:collapse; } table.AutoAnnotatorWithBorder { border:1px solid black; width:100%; border-collapse:collapse; } td.AutoAnnotatorOuterAmino { border:0px solid black; width:20% } td.AutoAnnotatorInnerAmino { border:1px solid black; width:50% } td.AutoAnnotatorAminoCountingOuter { border:1px solid black; width:40%; } td.AutoAnnotatorBiochemParOuter { border:1px solid black; width:60%; } td.AutoAnnotatorAminoCountingInner1 { width: 7.5% } td.AutoAnnotatorAminoCountingInner2 { width:62.5% } td.AutoAnnotatorAminoCountingInner3 { width:30% } td.AutoAnnotatorBiochemParInner1 { width: 5% } td.AutoAnnotatorBiochemParInner2 { width:55% } td.AutoAnnotatorBiochemParInner3 { width:40% } td.AutoAnnotatorCodonUsage1 { width: 3% } td.AutoAnnotatorCodonUsage2 { width:14.2% } td.AutoAnnotatorCodonUsage3 { width:13.8% } td.AutoAnnotatorAlignment1 { width: 3% } td.AutoAnnotatorAlignment2 { width: 10% } td.AutoAnnotatorAlignment3 { width: 87% } td.AutoAnnotatorLocalizationOuter {border:1px solid black; width:40%} td.AutoAnnotatorGOOuter {border:1px solid black; width:60%} td.AutoAnnotatorLocalization1 { width: 7.5% } td.AutoAnnotatorLocalization2 { width: 22.5% } td.AutoAnnotatorLocalization3 { width: 70% } td.AutoAnnotatorGO1 { width: 5% } td.AutoAnnotatorGO2 { width: 35% } td.AutoAnnotatorGO3 { width: 60% } td.AutoAnnotatorPredFeat1 { width:3% } td.AutoAnnotatorPredFeat2a { width:27% } td.AutoAnnotatorPredFeat3 { width:70% } div.AutoAnnotator_trans { position:absolute; background:rgb(11,140,143); background-color:rgba(11,140,143, 0.8); height:5px; top:100px; } div.AutoAnnotator_sec_helix { position:absolute; background:rgb(102,0,102); background-color:rgba(102,0,102, 0.8); height:5px; top:110px; } div.AutoAnnotator_sec_strand { position:absolute; background:rgb(245,170,26); background-color:rgba(245,170,26, 1); height:5px; top:110px; } div.AutoAnnotator_acc_buried { position:absolute; background:rgb(89,168,15); background-color:rgba(89,168,15, 0.8); height:5px; top:120px; } div.AutoAnnotator_acc_exposed { position:absolute; background:rgb(0, 0, 255); background-color:rgba(0, 0, 255, 0.8); height:5px; top:120px; } div.AutoAnnotator_dis { position:absolute; text-align:center; font-family:Arial,Helvetica,sans-serif; background:rgb(255, 200, 0); background-color:rgba(255, 200, 0, 1); height:16px; width:16px; top:80px; border-radius:50%; } </style><div id='AutoAnnotator_container_1381266044751'><table id="AutoAnnotator"><tr><!-- Time stamp in ms since 1/1/1970 1381266044751 --><th id="AutoAnnotatorHeader" colspan="2">Protein data table for BioBrick <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_<!------------------------Enter BioBrick number here------------------------>">BBa_<!------------------------Enter BioBrick number here------------------------></a> automatically created by the <a href="http://2013.igem.org/Team:TU-Munich/Results/AutoAnnotator">BioBrick-AutoAnnotator</a> version 1.0</th></tr><tr><td class="AutoAnnotator1col" colspan="2"><strong>Nucleotide sequence</strong> in <strong>RFC 25</strong>, so ATGGCCGGC and ACCGGT were added (in italics) to the 5' and 3' ends: (underlined part encodes the protein)<br><span class="AutoAnnotatorSequence"> <u><i>ATGGCCGGC</i>CCTGGACAA ... CTCTACAAA<i>ACCGGT</i></u></span><br> <strong>ORF</strong> from nucleotide position -8 to 939 (excluding stop-codon)</td></tr><tr><td class="AutoAnnotator1col" colspan="2"><strong>Amino acid sequence:</strong> (RFC 25 scars in shown in bold, other sequence features underlined; both given below)<br><span class="AutoAnnotatorSequence"><table class="AutoAnnotatorNoBorder"><tr><td class="AutoAnnotatorSeqNum">1 <br>101 <br>201 <br>301 </td><td class="AutoAnnotatorSeqSeq">MAGPGQPPFPPPPPFTPPPPQTPNGASGENSTGAIAGGVAAGAALLFAAPAIGFAWWRRRRPIEA<b>TG</b>GGSGGGSG<b>TG</b>SKGEELFTGVVPILVELDGDVNG<br>HKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIE<br>LKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLSPDNHYLSTQSALSKDPNEKRDHMVLLEFV<br>TAAGITHGMDELYKTG*</td></tr></table></span></td></tr><tr><td class="AutoAnnotator1col" colspan="2"><strong>Sequence features:</strong> (with their position in the amino acid sequence, see the <a href="http://2013.igem.org/Team:TU-Munich/Results/Software/FeatureList">list of supported features</a>)<table class="AutoAnnotatorNoBorder"><tr><td class="AutoAnnotatorSeqFeat1"></td><td class="AutoAnnotatorSeqFeat2a">RFC25 scar (shown in bold): </td><td class="AutoAnnotatorSeqFeat3">66 to 67, 76 to 77</td></tr></table></td></tr><tr><td class="AutoAnnotator1col" colspan="2"><strong>Amino acid composition:</strong><table class="AutoAnnotatorNoBorder"><tr><td class="AutoAnnotatorOuterAmino"><table class="AutoAnnotatorWithBorder"><tr><td class="AutoAnnotatorInnerAmino">Ala (A)</td><td class="AutoAnnotatorInnerAmino">21 (6.6%)</td></tr><tr><td class="AutoAnnotatorInnerAmino">Arg (R)</td><td class="AutoAnnotatorInnerAmino">10 (3.2%)</td></tr><tr><td class="AutoAnnotatorInnerAmino">Asn (N)</td><td class="AutoAnnotatorInnerAmino">15 (4.7%)</td></tr><tr><td class="AutoAnnotatorInnerAmino">Asp (D)</td><td class="AutoAnnotatorInnerAmino">18 (5.7%)</td></tr></table></td><td class="AutoAnnotatorOuterAmino"><table class="AutoAnnotatorWithBorder"><tr><td class="AutoAnnotatorInnerAmino">Cys (C)</td><td class="AutoAnnotatorInnerAmino">2 (0.6%)</td></tr><tr><td class="AutoAnnotatorInnerAmino">Gln (Q)</td><td class="AutoAnnotatorInnerAmino">10 (3.2%)</td></tr><tr><td class="AutoAnnotatorInnerAmino">Glu (E)</td><td class="AutoAnnotatorInnerAmino">18 (5.7%)</td></tr><tr><td class="AutoAnnotatorInnerAmino">Gly (G)</td><td class="AutoAnnotatorInnerAmino">40 (12.7%)</td></tr></table></td><td class="AutoAnnotatorOuterAmino"><table class="AutoAnnotatorWithBorder"><tr><td class="AutoAnnotatorInnerAmino">His (H)</td><td class="AutoAnnotatorInnerAmino">10 (3.2%)</td></tr><tr><td class="AutoAnnotatorInnerAmino">Ile (I)</td><td class="AutoAnnotatorInnerAmino">15 (4.7%)</td></tr><tr><td class="AutoAnnotatorInnerAmino">Leu (L)</td><td class="AutoAnnotatorInnerAmino">21 (6.6%)</td></tr><tr><td class="AutoAnnotatorInnerAmino">Lys (K)</td><td class="AutoAnnotatorInnerAmino">20 (6.3%)</td></tr></table></td><td class="AutoAnnotatorOuterAmino"><table class="AutoAnnotatorWithBorder"><tr><td class="AutoAnnotatorInnerAmino">Met (M)</td><td class="AutoAnnotatorInnerAmino">6 (1.9%)</td></tr><tr><td class="AutoAnnotatorInnerAmino">Phe (F)</td><td class="AutoAnnotatorInnerAmino">16 (5.1%)</td></tr><tr><td class="AutoAnnotatorInnerAmino">Pro (P)</td><td class="AutoAnnotatorInnerAmino">25 (7.9%)</td></tr><tr><td class="AutoAnnotatorInnerAmino">Ser (S)</td><td class="AutoAnnotatorInnerAmino">15 (4.7%)</td></tr></table></td><td class="AutoAnnotatorOuterAmino"><table class="AutoAnnotatorWithBorder"><tr><td class="AutoAnnotatorInnerAmino">Thr (T)</td><td class="AutoAnnotatorInnerAmino">22 (7.0%)</td></tr><tr><td class="AutoAnnotatorInnerAmino">Trp (W)</td><td class="AutoAnnotatorInnerAmino">3 (0.9%)</td></tr><tr><td class="AutoAnnotatorInnerAmino">Tyr (Y)</td><td class="AutoAnnotatorInnerAmino">11 (3.5%)</td></tr><tr><td class="AutoAnnotatorInnerAmino">Val (V)</td><td class="AutoAnnotatorInnerAmino">18 (5.7%)</td></tr></table></td></tr></table></td></tr><tr><td class="AutoAnnotatorAminoCountingOuter"><strong>Amino acid counting</strong><table class="AutoAnnotatorNoBorder"><tr><td class="AutoAnnotatorAminoCountingInner1"></td><td class="AutoAnnotatorAminoCountingInner2">Total number:</td><td class="AutoAnnotatorAminoCountingInner3">316</td></tr><tr><td class="AutoAnnotatorAminoCountingInner1"></td><td class="AutoAnnotatorAminoCountingInner2">Positively charged (Arg+Lys):</td><td class="AutoAnnotatorAminoCountingInner3">30 (9.5%)</td></tr><tr><td class="AutoAnnotatorAminoCountingInner1"></td><td class="AutoAnnotatorAminoCountingInner2">Negatively charged (Asp+Glu):</td><td class="AutoAnnotatorAminoCountingInner3">36 (11.4%)</td></tr><tr><td class="AutoAnnotatorAminoCountingInner1"></td><td class="AutoAnnotatorAminoCountingInner2">Aromatic (Phe+His+Try+Tyr):</td><td class="AutoAnnotatorAminoCountingInner3">40 (12.7%)</td></tr></table></td><td class="AutoAnnotatorBiochemParOuter"><strong>Biochemical parameters</strong><table class="AutoAnnotatorNoBorder"><tr><td class="AutoAnnotatorBiochemParInner1"></td><td class="AutoAnnotatorBiochemParInner2">Atomic composition:</td><td class="AutoAnnotatorBiochemParInner3">C<sub>1531</sub>H<sub>2342</sub>N<sub>414</sub>O<sub>462</sub>S<sub>8</sub></td></tr><tr><td class="AutoAnnotatorBiochemParInner1"></td><td class="AutoAnnotatorBiochemParInner2">Molecular mass [Da]:</td><td class="AutoAnnotatorBiochemParInner3">34196.4</td></tr><tr><td class="AutoAnnotatorBiochemParInner1"></td><td class="AutoAnnotatorBiochemParInner2">Theoretical pI:</td><td class="AutoAnnotatorBiochemParInner3">5.93</td></tr><tr><td class="AutoAnnotatorBiochemParInner1"></td><td class="AutoAnnotatorBiochemParInner2">Extinction coefficient at 280 nm [M<sup>-1</sup> cm<sup>-1</sup>]:</td><td class="AutoAnnotatorBiochemParInner3">32890 / 33015 (all Cys red/ox)</td></tr></table></td></tr><tr><td class="AutoAnnotator1col" colspan="2"><strong>Plot for hydrophobicity, charge, predicted secondary structure, solvent accessability, transmembrane helices and disulfid bridges</strong> <input type='button' id='hydrophobicity_charge_button' onclick='show_or_hide_plot_1381266044751()' value='Show'><span id="hydrophobicity_charge_explanation"></span><div id="hydrophobicity_charge_container" style='display:none'><div id="hydrophobicity_charge_placeholder0" style="width:100%;height:150px"></div><div id="hydrophobicity_charge_placeholder1" style="width:100%;height:150px"></div></div></td></tr><tr><td class="AutoAnnotator1col" colspan="2"><strong>Codon usage</strong><table class="AutoAnnotatorNoBorder"><tr><td class="AutoAnnotatorCodonUsage1"></td><td class="AutoAnnotatorCodonUsage2">Organism:</td><td class="AutoAnnotatorCodonUsage3"><i>E. coli</i></td><td class="AutoAnnotatorCodonUsage3"><i>B. subtilis</i></td><td class="AutoAnnotatorCodonUsage3"><i>S. cerevisiae</i></td><td class="AutoAnnotatorCodonUsage3"><i>A. thaliana</i></td><td class="AutoAnnotatorCodonUsage3"><i>P. patens</i></td><td class="AutoAnnotatorCodonUsage3">Mammals</td></tr><tr><td class="AutoAnnotatorCodonUsage1"></td><td class="AutoAnnotatorCodonUsage2">Codon quality (<a href="http://en.wikipedia.org/wiki/Codon_Adaptation_Index">CAI</a>):</td><td class="AutoAnnotatorCodonUsage3">good (0.73)</td><td class="AutoAnnotatorCodonUsage3">good (0.75)</td><td class="AutoAnnotatorCodonUsage3">good (0.76)</td><td class="AutoAnnotatorCodonUsage3">excellent (0.84)</td><td class="AutoAnnotatorCodonUsage3">excellent (0.81)</td><td class="AutoAnnotatorCodonUsage3">good (0.71)</td></tr></table></td></tr><tr><td class="AutoAnnotator1col" colspan="2"><strong>Alignments</strong> (obtained from <a href='http://predictprotein.org'>PredictProtein.org</a>)<table class="AutoAnnotatorNoBorder"><tr><td class="AutoAnnotatorAlignment1"></td><td class="AutoAnnotatorAlignment2">SwissProt:</td><td class="AutoAnnotatorAlignment3"><a href='http://www.uniprot.org/uniprot/P42212'>P42212</a> (98% identity on 238 AAs), <a href='http://www.uniprot.org/uniprot/Q9U6Y3'>Q9U6Y3</a> (25% identity on 217 AAs)</td></tr><tr><td class="AutoAnnotatorAlignment1"></td><td class="AutoAnnotatorAlignment2">TrEML:</td><td class="AutoAnnotatorAlignment3"><a href='http://www.uniprot.org/uniprot/Q71RY9'>Q71RY9</a> (98% identity on 238 AAs), <a href='http://www.uniprot.org/uniprot/B6F2F5'>B6F2F5</a> (97% identity on 241 AAs)</td></tr><tr><td class="AutoAnnotatorAlignment1"></td><td class="AutoAnnotatorAlignment2">PDB:</td><td class="AutoAnnotatorAlignment3"><a href='http://www.rcsb.org/pdb/explore/explore.do?structureId=1gfl'>1gfl</a> (98% identity on 229 AAs), <a href='http://www.rcsb.org/pdb/explore/explore.do?structureId=2y0g'>2y0g</a> (98% identity on 226 AAs)</td></tr></table></td></tr><tr><th id='AutoAnnotatorHeader' colspan="2"><strong>Predictions</strong> (obtained from <a href='http://predictprotein.org'>PredictProtein.org</a>)</th></tr><tr><td class="AutoAnnotatorLocalizationOuter"><strong>Subcellular Localization</strong> (reliability in brackets)<table class="AutoAnnotatorNoBorder"><tr><td class="AutoAnnotatorLocalization1"></td><td class="AutoAnnotatorLocalization2">Archaea:</td><td class="AutoAnnotatorLocalization3">secreted (100%)</td></tr><tr><td class="AutoAnnotatorLocalization1"></td><td class="AutoAnnotatorLocalization2">Bacteria:</td><td class="AutoAnnotatorLocalization3">secreted (74%)</td></tr><tr><td class="AutoAnnotatorLocalization1"></td><td class="AutoAnnotatorLocalization2">Eukarya:</td><td class="AutoAnnotatorLocalization3">cytosol (44%)</td></tr></table></td><td class="AutoAnnotatorGOOuter"><strong>Gene Ontology</strong> (reliability in brackets)<br><table class="AutoAnnotatorNoBorder"><tr><td class='AutoAnnotatorGO1'></td><td class='AutoAnnotatorGO2'>Molecular Function Ontology:</td><td class='AutoAnnotatorGO3'> - </td></tr><tr><td class='AutoAnnotatorGO1'></td><td class='AutoAnnotatorGO2'>Biological Process Ontology:</td><td class='AutoAnnotatorGO3'><a href='http://amigo.geneontology.org/cgi-bin/amigo/term_details?term=GO:0018298'>GO:0018298</a> (44%), <a href='http://amigo.geneontology.org/cgi-bin/amigo/term_details?term=GO:0008218'>GO:0008218</a> (31%)</td></tr><tr><td class='AutoAnnotatorGO1'> </td><td class='AutoAnnotatorGO2'> </td><td class='AutoAnnotatorGO3'> </td></tr></table></td></tr><tr><td class="AutoAnnotator1col" colspan="2"><strong>Predicted features:</strong><table class="AutoAnnotatorNoBorder"><tr><td class="AutoAnnotatorPredFeat1"></td><td class="AutoAnnotatorPredFeat2a">Disulfid bridges:</td><td class="AutoAnnotatorPredFeat3"> - </td></tr><tr><td class="AutoAnnotatorPredFeat1"></td><td class="AutoAnnotatorPredFeat2a">Transmembrane helices:</td><td class="AutoAnnotatorPredFeat3">37 to 54 going inwards</td></tr></table></td></tr><tr><td class="AutoAnnotator1col" colspan="2"> The BioBrick-AutoAnnotator was created by <a href="http://2013.igem.org/Team:TU-Munich">TU-Munich 2013</a> iGEM team. For more information please see the <a href="http://2013.igem.org/Team:TU-Munich/Results/Software">documentation</a>.<br>If you have any questions, comments or suggestions, please leave us a <a href="http://2013.igem.org/Team:TU-Munich/Results/AutoAnnotator">comment</a>.</td></tr></table></div><br><!-- IMPORTANT: DON'T REMOVE THIS LINE, OTHERWISE NOT SUPPORTED FOR IE BEFORE 9 --><!--[if lte IE 8]><script language="javascript" type="text/javascript" src="excanvas.min.js"></script><![endif]--><script type='text/javascript' src='http://code.jquery.com/jquery-1.10.0.min.js'></script><script type='text/javascript' src='http://2013.igem.org/Team:TU-Munich/Flot.js?action=raw&ctype=text/js'></script><script>function show_or_hide_plot_1381266044751(){hydrophobicity_datapoints = [[2.5,0.26],[3.5,-0.82],[4.5,-1.50],[5.5,-1.74],[6.5,-0.86],[7.5,-1.10],[8.5,-0.72],[9.5,-0.72],[10.5,-0.72],[11.5,-1.60],[12.5,-0.72],[13.5,-0.54],[14.5,-0.54],[15.5,-0.54],[16.5,-0.54],[17.5,-1.42],[18.5,-1.98],[19.5,-1.80],[20.5,-1.80],[21.5,-2.18],[22.5,-1.94],[23.5,-0.88],[24.5,-0.90],[25.5,-0.66],[26.5,-0.66],[27.5,-1.28],[28.5,-1.80],[29.5,-1.78],[30.5,-1.78],[31.5,-0.72],[32.5,0.88],[33.5,1.40],[34.5,1.46],[35.5,1.46],[36.5,1.94],[37.5,1.40],[38.5,1.40],[39.5,1.40],[40.5,1.84],[41.5,1.36],[42.5,1.76],[43.5,2.16],[44.5,2.80],[45.5,2.80],[46.5,2.80],[47.5,1.72],[48.5,1.32],[49.5,1.66],[50.5,1.22],[51.5,1.42],[52.5,2.10],[53.5,1.56],[54.5,0.48],[55.5,-0.34],[56.5,-1.80],[57.5,-3.06],[58.5,-3.78],[59.5,-3.92],[60.5,-2.12],[61.5,-1.92],[62.5,-0.66],[63.5,0.10],[64.5,0.34],[65.5,-0.64],[66.5,-0.02],[67.5,-0.54],[68.5,-0.48],[69.5,-0.48],[70.5,-0.48],[71.5,-0.56],[72.5,-0.48],[73.5,-0.54],[74.5,-0.54],[75.5,-0.62],[76.5,-1.24],[77.5,-1.24],[78.5,-1.80],[79.5,-2.42],[80.5,-1.50],[81.5,-0.16],[82.5,-0.22],[83.5,0.40],[84.5,1.94],[85.5,2.02],[86.5,1.14],[87.5,2.18],[88.5,3.02],[89.5,3.02],[90.5,1.48],[91.5,2.56],[92.5,0.96],[93.5,0.12],[94.5,-1.42],[95.5,0.12],[96.5,-1.34],[97.5,-0.72],[98.5,-1.28],[99.5,-1.36],[100.5,-1.64],[101.5,-1.10],[102.5,-0.18],[103.5,0.30],[104.5,1.00],[105.5,-0.26],[106.5,-0.18],[107.5,-1.72],[108.5,-1.64],[109.5,-2.26],[110.5,-1.20],[111.5,-1.26],[112.5,-0.82],[113.5,-0.82],[114.5,-0.90],[115.5,-0.50],[116.5,-0.50],[117.5,0.52],[118.5,-0.18],[119.5,1.16],[120.5,1.30],[121.5,1.94],[122.5,1.04],[123.5,1.68],[124.5,1.04],[125.5,-0.64],[126.5,-0.38],[127.5,-0.56],[128.5,0.42],[129.5,0.18],[130.5,0.78],[131.5,-0.30],[132.5,-0.12],[133.5,-0.20],[134.5,0.96],[135.5,1.00],[136.5,1.18],[137.5,2.08],[138.5,1.18],[139.5,0.08],[140.5,0.14],[141.5,1.12],[142.5,-0.34],[143.5,0.30],[144.5,1.12],[145.5,1.04],[146.5,-0.70],[147.5,-0.26],[148.5,-1.08],[149.5,-2.34],[150.5,-2.82],[151.5,-1.54],[152.5,-2.06],[153.5,-2.44],[154.5,-2.38],[155.5,-2.44],[156.5,-2.26],[157.5,-0.92],[158.5,-1.00],[159.5,-0.52],[160.5,0.54],[161.5,0.36],[162.5,-0.52],[163.5,-0.44],[164.5,-0.36],[165.5,-0.98],[166.5,-0.52],[167.5,-0.90],[168.5,-0.90],[169.5,-1.72],[170.5,-1.60],[171.5,-1.54],[172.5,-0.28],[173.5,0.98],[174.5,1.10],[175.5,0.54],[176.5,-1.06],[177.5,-1.70],[178.5,-2.96],[179.5,-2.44],[180.5,-2.52],[181.5,-1.96],[182.5,-2.78],[183.5,-1.72],[184.5,-2.16],[185.5,-0.54],[186.5,-1.18],[187.5,0.28],[188.5,-0.78],[189.5,-0.16],[190.5,-1.70],[191.5,-1.06],[192.5,-0.86],[193.5,0.68],[194.5,0.06],[195.5,-0.14],[196.5,0.90],[197.5,-0.56],[198.5,-0.64],[199.5,-0.72],[200.5,0.10],[201.5,0.10],[202.5,0.10],[203.5,-0.10],[204.5,-0.10],[205.5,-0.72],[206.5,-2.32],[207.5,-1.70],[208.5,-2.96],[209.5,-1.28],[210.5,0.18],[211.5,0.80],[212.5,0.24],[213.5,0.16],[214.5,0.02],[215.5,-1.44],[216.5,-1.62],[217.5,-1.68],[218.5,-1.16],[219.5,-2.62],[220.5,-2.08],[221.5,-2.46],[222.5,-2.46],[223.5,-1.36],[224.5,-0.92],[225.5,0.14],[226.5,1.16],[227.5,2.22],[228.5,0.68],[229.5,0.16],[230.5,-1.44],[231.5,-2.60],[232.5,-3.66],[233.5,-3.04],[234.5,-1.36],[235.5,-1.44],[236.5,0.18],[237.5,0.18],[238.5,0.82],[239.5,-0.86],[240.5,0.82],[241.5,-0.92],[242.5,-0.86],[243.5,-2.12],[244.5,-0.44],[245.5,-2.04],[246.5,-1.84],[247.5,-1.28],[248.5,-0.74],[249.5,-0.80],[250.5,-0.80],[251.5,0.66],[252.5,1.10],[253.5,0.56],[254.5,-0.92],[255.5,-0.48],[256.5,-1.94],[257.5,-3.00],[258.5,-3.00],[259.5,-2.50],[260.5,-2.56],[261.5,-0.96],[262.5,-0.34],[263.5,-0.34],[264.5,-0.28],[265.5,-0.28],[266.5,-0.34],[267.5,0.50],[268.5,1.04],[269.5,0.80],[270.5,0.42],[271.5,-1.12],[272.5,-2.52],[273.5,-2.62],[274.5,-1.54],[275.5,-1.00],[276.5,-0.44],[277.5,-0.50],[278.5,-0.40],[279.5,-0.80],[280.5,0.12],[281.5,0.10],[282.5,0.02],[283.5,-0.52],[284.5,-1.20],[285.5,-2.66],[286.5,-3.20],[287.5,-3.20],[288.5,-3.40],[289.5,-3.78],[290.5,-3.72],[291.5,-2.64],[292.5,-1.02],[293.5,0.64],[294.5,2.10],[295.5,2.04],[296.5,2.22],[297.5,2.22],[298.5,1.32],[299.5,0.92],[300.5,1.98],[301.5,1.34],[302.5,1.40],[303.5,1.40],[304.5,0.40],[305.5,-0.04],[306.5,0.42],[307.5,-1.18],[308.5,-1.74],[309.5,-0.34],[310.5,-0.52],[311.5,-1.68],[312.5,-1.12],[313.5,-0.50]];charge_datapoints = [[2.5,0.00],[3.5,0.00],[4.5,0.00],[5.5,0.00],[6.5,0.00],[7.5,0.00],[8.5,0.00],[9.5,0.00],[10.5,0.00],[11.5,0.00],[12.5,0.00],[13.5,0.00],[14.5,0.00],[15.5,0.00],[16.5,0.00],[17.5,0.00],[18.5,0.00],[19.5,0.00],[20.5,0.00],[21.5,0.00],[22.5,0.00],[23.5,0.00],[24.5,0.00],[25.5,0.00],[26.5,-0.20],[27.5,-0.20],[28.5,-0.20],[29.5,-0.20],[30.5,-0.20],[31.5,0.00],[32.5,0.00],[33.5,0.00],[34.5,0.00],[35.5,0.00],[36.5,0.00],[37.5,0.00],[38.5,0.00],[39.5,0.00],[40.5,0.00],[41.5,0.00],[42.5,0.00],[43.5,0.00],[44.5,0.00],[45.5,0.00],[46.5,0.00],[47.5,0.00],[48.5,0.00],[49.5,0.00],[50.5,0.00],[51.5,0.00],[52.5,0.00],[53.5,0.00],[54.5,0.00],[55.5,0.20],[56.5,0.40],[57.5,0.60],[58.5,0.80],[59.5,0.80],[60.5,0.60],[61.5,0.20],[62.5,0.00],[63.5,-0.20],[64.5,-0.20],[65.5,-0.20],[66.5,0.00],[67.5,0.00],[68.5,0.00],[69.5,0.00],[70.5,0.00],[71.5,0.00],[72.5,0.00],[73.5,0.00],[74.5,0.00],[75.5,0.00],[76.5,0.20],[77.5,0.20],[78.5,0.00],[79.5,-0.20],[80.5,-0.20],[81.5,-0.40],[82.5,-0.40],[83.5,-0.20],[84.5,0.00],[85.5,0.00],[86.5,0.00],[87.5,0.00],[88.5,0.00],[89.5,0.00],[90.5,-0.20],[91.5,-0.20],[92.5,-0.40],[93.5,-0.40],[94.5,-0.60],[95.5,-0.40],[96.5,-0.40],[97.5,-0.20],[98.5,-0.10],[99.5,0.30],[100.5,0.30],[101.5,0.30],[102.5,0.30],[103.5,0.20],[104.5,0.00],[105.5,-0.20],[106.5,-0.20],[107.5,-0.40],[108.5,-0.40],[109.5,-0.60],[110.5,-0.40],[111.5,-0.40],[112.5,-0.20],[113.5,-0.20],[114.5,0.20],[115.5,0.20],[116.5,0.20],[117.5,0.20],[118.5,0.40],[119.5,0.20],[120.5,0.20],[121.5,0.20],[122.5,0.20],[123.5,-0.00],[124.5,-0.00],[125.5,0.20],[126.5,0.20],[127.5,0.20],[128.5,0.20],[129.5,0.20],[130.5,-0.00],[131.5,-0.00],[132.5,-0.00],[133.5,-0.00],[134.5,-0.00],[135.5,-0.00],[136.5,-0.00],[137.5,-0.00],[138.5,-0.00],[139.5,-0.00],[140.5,-0.00],[141.5,-0.00],[142.5,-0.00],[143.5,-0.00],[144.5,-0.00],[145.5,-0.00],[146.5,0.20],[147.5,0.20],[148.5,0.20],[149.5,-0.00],[150.5,0.10],[151.5,-0.10],[152.5,0.10],[153.5,0.10],[154.5,0.40],[155.5,0.10],[156.5,0.10],[157.5,-0.10],[158.5,0.10],[159.5,-0.00],[160.5,0.20],[161.5,0.20],[162.5,0.20],[163.5,-0.20],[164.5,-0.20],[165.5,-0.20],[166.5,-0.20],[167.5,-0.20],[168.5,-0.20],[169.5,-0.00],[170.5,-0.00],[171.5,-0.00],[172.5,-0.00],[173.5,0.20],[174.5,0.20],[175.5,-0.00],[176.5,-0.20],[177.5,-0.20],[178.5,-0.20],[179.5,-0.40],[180.5,-0.00],[181.5,0.20],[182.5,0.40],[183.5,0.40],[184.5,0.20],[185.5,-0.00],[186.5,0.20],[187.5,-0.00],[188.5,-0.20],[189.5,-0.00],[190.5,-0.20],[191.5,-0.40],[192.5,-0.40],[193.5,-0.20],[194.5,-0.20],[195.5,0.20],[196.5,0.20],[197.5,-0.00],[198.5,-0.00],[199.5,0.20],[200.5,-0.00],[201.5,-0.00],[202.5,-0.00],[203.5,-0.00],[204.5,-0.00],[205.5,-0.20],[206.5,-0.40],[207.5,-0.20],[208.5,-0.20],[209.5,-0.40],[210.5,-0.20],[211.5,-0.00],[212.5,0.10],[213.5,0.30],[214.5,0.30],[215.5,0.10],[216.5,0.10],[217.5,-0.00],[218.5,-0.20],[219.5,-0.20],[220.5,-0.00],[221.5,0.10],[222.5,0.10],[223.5,0.10],[224.5,0.10],[225.5,0.10],[226.5,-0.00],[227.5,-0.00],[228.5,-0.20],[229.5,-0.00],[230.5,-0.00],[231.5,0.20],[232.5,0.20],[233.5,0.40],[234.5,0.20],[235.5,0.40],[236.5,0.20],[237.5,0.20],[238.5,0.20],[239.5,0.40],[240.5,0.20],[241.5,0.40],[242.5,0.50],[243.5,0.50],[244.5,0.30],[245.5,0.10],[246.5,-0.30],[247.5,-0.40],[248.5,-0.40],[249.5,-0.40],[250.5,-0.20],[251.5,-0.00],[252.5,-0.00],[253.5,-0.20],[254.5,-0.10],[255.5,-0.10],[256.5,-0.10],[257.5,-0.10],[258.5,0.10],[259.5,-0.00],[260.5,-0.00],[261.5,-0.00],[262.5,-0.00],[263.5,-0.20],[264.5,-0.20],[265.5,-0.20],[266.5,-0.20],[267.5,-0.20],[268.5,-0.00],[269.5,-0.00],[270.5,-0.20],[271.5,-0.20],[272.5,-0.10],[273.5,-0.10],[274.5,-0.10],[275.5,0.10],[276.5,0.10],[277.5,-0.00],[278.5,-0.00],[279.5,-0.00],[280.5,-0.00],[281.5,-0.00],[282.5,0.20],[283.5,-0.00],[284.5,-0.00],[285.5,-0.00],[286.5,-0.20],[287.5,-0.20],[288.5,0.20],[289.5,-0.00],[290.5,0.10],[291.5,0.30],[292.5,0.10],[293.5,-0.10],[294.5,0.10],[295.5,-0.20],[296.5,-0.20],[297.5,-0.20],[298.5,-0.20],[299.5,-0.20],[300.5,-0.00],[301.5,-0.00],[302.5,-0.00],[303.5,-0.00],[304.5,0.10],[305.5,0.10],[306.5,0.10],[307.5,-0.10],[308.5,-0.30],[309.5,-0.40],[310.5,-0.40],[311.5,-0.20],[312.5,-0.00],[313.5,0.20]];dis_datapoints = [];trans_datapoints = [[37,54,'inwards']];sec_helix_datapoints = [[39,53],[133,141],[160,162],[310,313]];sec_strand_datapoints = [[32,38],[54,58],[65,68],[82,95],[97,98],[102,107],[117,124],[145,147],[170,177],[181,191],[194,200],[201,203],[215,216],[224,230],[236,247],[252,259],[276,284],[293,303]];acc_exposed_datapoints = [[1,2],[4,4],[8,8],[10,11],[14,14],[16,21],[23,29],[61,62],[64,64],[70,73],[75,76],[81,82],[85,86],[95,95],[97,97],[99,99],[102,102],[108,108],[110,110],[112,112],[115,115],[117,117],[126,126],[128,128],[149,149],[152,153],[155,156],[158,158],[161,161],[165,166],[191,192],[205,205],[208,209],[216,216],[218,218],[220,220],[222,222],[232,234],[248,249],[251,251],[260,262],[265,268],[270,270],[273,273],[285,292],[308,308],[310,311],[314,316]];acc_buried_datapoints = [[32,57],[60,60],[66,67],[77,78],[80,80],[84,84],[88,88],[90,90],[92,92],[94,94],[96,96],[98,98],[103,103],[105,105],[107,107],[109,109],[111,111],[113,113],[116,116],[118,118],[120,120],[122,122],[124,124],[129,141],[143,144],[146,147],[151,151],[159,160],[163,164],[167,169],[173,174],[176,176],[178,178],[180,182],[184,184],[186,186],[188,188],[190,190],[194,195],[199,199],[201,201],[203,204],[206,206],[210,210],[212,213],[224,224],[226,226],[228,228],[237,237],[239,239],[241,241],[243,245],[247,247],[253,253],[255,255],[257,257],[269,269],[271,272],[274,277],[279,279],[281,281],[283,283],[294,294],[296,296],[298,298],[300,300],[302,302],[309,309],[312,312]];flot_plot_options = []; flot_plot_options[0] = {grid: {borderWidth: {top: 0,right: 0,bottom: 0,left: 0}},legend: {show: false},xaxes: [{show: true,min: 0,max: 200,ticks: [[0.5, '1'], [24.5, '25'], [49.5, '50'], [74.5, '75'], [99.5, '100'], [124.5, '125'], [149.5, '150'], [174.5, '175'], [199.5, '200']],tickLength: -5}],yaxes: [{show: true,ticks: [[0, '0'], [4.5,'hydro-<br>phobic '], [-4.5,'hydro-<br>philic ']],min: -4.5,max: +4.5,font: {size: 12,lineHeight: 14,style: 'italic',weight: 'bold',family: 'sans-serif',variant: 'small-caps',color: 'rgba(100,149,237,1)'}},{show: true,ticks: [[0, ''], [1,'positive<br> charge'], [-1,'negative<br> charge']],position: 'right',min: -1,max: 1,font: {size: 12,lineHeight: 14,style: 'italic',weight: 'bold',family: 'sans-serif',variant: 'small-caps',color: 'rgba(255,99,71,1)'}}]};number_of_plots = 2;for ( plot_num = 1 ; plot_num < number_of_plots ; plot_num ++){flot_plot_options[plot_num] = $.extend(true, {} ,flot_plot_options[0]);flot_plot_options[plot_num].xaxes = [{min: plot_num*200,max: (plot_num + 1)*200,ticks: [ [plot_num*200 + 0.5, (plot_num*200 + 1).toString()], [plot_num*200 + 24.5, (plot_num*200 + 25).toString()], [plot_num*200 + 49.5, (plot_num*200 + 50).toString()], [plot_num*200 + 74.5, (plot_num*200 + 75).toString()], [plot_num*200 + 99.5, (plot_num*200 + 100).toString()], [plot_num*200 + 124.5, (plot_num*200 + 125).toString()], [plot_num*200 + 149.5, (plot_num*200 + 150).toString()], [plot_num*200 + 174.5, (plot_num*200 + 175).toString()], [plot_num*200 + 199.5, (plot_num*200 + 200).toString()] ],tickLength: -5}];};try {if( $('#AutoAnnotator_container_1381266044751 #hydrophobicity_charge_button').val() =='Show' ){$('#AutoAnnotator_container_1381266044751 #hydrophobicity_charge_container').css('display','block');$('#AutoAnnotator_container_1381266044751 #hydrophobicity_charge_button').val('Hide');var description_html = '<div id=\'AutoAnnotator_plot_selectors\'>';description_html = description_html + '<br> <input type=\'checkbox\' id=\'hydrophobicity_checkbox\' checked=\'checked\'> Moving average over 5 amino acids for hydrophobicity (<img src=\'https://static.igem.org/mediawiki/2013/e/e9/TUM13_hydrophobicity_icon.png\' alt=\'blue graph\' height=\'10\'></img>)';description_html = description_html + '<br> <input type=\'checkbox\' id=\'charge_checkbox\' checked=\'checked\'> Moving average over 5 amino acids for charge (<img src=\'https://static.igem.org/mediawiki/2013/3/3e/TUM13_charge_icon.png\' alt=\'red graph\' height=\'10\'></img>)';description_html = description_html + '<br> <input type=\'checkbox\' id=\'dis_checkbox\' checked=\'checked\'> Predicted disulfid bridges (<img src=\'https://static.igem.org/mediawiki/2013/2/28/TUM13_dis_icon.png\' alt=\'yellow circle\' height=\'10\'></img>) with the number of the bridge in the center';description_html = description_html + '<br> <input type=\'checkbox\' id=\'trans_checkbox\' checked=\'checked\'> Predicted transmembrane helices (<img src=\'https://static.igem.org/mediawiki/2013/7/78/TUM13_trans_icon.png\' alt=\'turquois bars\' height=\'10\'></img>)';description_html = description_html + '<br> <input type=\'checkbox\' id=\'sec_checkbox\' checked=\'checked\'> Predicted secondary structure: Helices (<img src=\'https://static.igem.org/mediawiki/2013/b/bf/TUM13_helix_icon.png\' alt=\'violet bars\' height=\'10\'></img>) and beta-strands (<img src=\'https://static.igem.org/mediawiki/2013/b/bf/TUM13_strand_icon.png\' alt=\'yellow bars\' height=\'10\'></img>)';description_html = description_html + '<br> <input type=\'checkbox\' id=\'acc_checkbox\' checked=\'checked\'> Predicted solvent accessability: Exposed (<img src=\'https://static.igem.org/mediawiki/2013/1/16/TUM13_exposed_icon.png\' alt=\'blue bars\' height=\'10\'></img>) and buried (<img src=\'https://static.igem.org/mediawiki/2013/0/0b/TUM13_buried_icon.png\' alt=\'green bars\' height=\'10\'></img>) residues';description_html = description_html + '<br></div>';$('#AutoAnnotator_container_1381266044751 #hydrophobicity_charge_explanation').html(description_html);plot_according_to_selectors_1381266044751();$('#AutoAnnotator_container_1381266044751 #AutoAnnotator_plot_selectors').find('input').click(plot_according_to_selectors_1381266044751);}else{$('#AutoAnnotator_container_1381266044751 #hydrophobicity_charge_container').css('display','none');$('#AutoAnnotator_container_1381266044751 #hydrophobicity_charge_button').val('Show');$('#AutoAnnotator_container_1381266044751 #hydrophobicity_charge_explanation').html('');}}catch(err){txt='There was an error with the button controlling the visibility of the plot.\n';txt=txt+'The originating error is:\n' + err + '\n\n';alert(txt);}};function plot_according_to_selectors_1381266044751(){try{var plot_datasets = [[],[]];if($('#AutoAnnotator_container_1381266044751 #hydrophobicity_checkbox').prop('checked') == true){plot_datasets[0] = { color: 'rgba(100,149,237,1)',data: hydrophobicity_datapoints,label: 'Hydrophobicity',lines: { show: true, fill: true, fillColor: 'rgba(100,149,237,0.1)' },yaxis: 1};}if($('#AutoAnnotator_container_1381266044751 #charge_checkbox').prop('checked') == true){plot_datasets[1] = {color: 'rgba(255,99,71,1)',data: charge_datapoints,label: 'Charge',lines: { show: true, fill: true, fillColor: 'rgba(255,99,71,0.1)' },yaxis: 2};}for (plot_num = 0 ; plot_num < number_of_plots ; plot_num ++){$.plot('#AutoAnnotator_container_1381266044751 #hydrophobicity_charge_placeholder'+ plot_num.toString(), plot_datasets, flot_plot_options[plot_num] );}var screen_width = $('canvas.flot-base').width(); var pos_of_first_tick = 46;var pos_of_last_tick = screen_width - 51;var tick_diff = (screen_width - 97)/199;if($('#AutoAnnotator_container_1381266044751 #dis_checkbox').prop('checked') == true){for ( j = 0 ; j < dis_datapoints.length ; j++ ){$('#AutoAnnotator_container_1381266044751 #hydrophobicity_charge_placeholder' + Math.floor((dis_datapoints[j][0] - 1)/200) ).append('<div class=\'AutoAnnotator_dis\' style=\'left:' + ((pos_of_first_tick - 8 + (dis_datapoints[j][0] - 1)*tick_diff - Math.floor((dis_datapoints[j][0] - 1)/200)*200*tick_diff).toFixed(0)).toString() + 'px;\'><b>' + (j+1) + '</b></div>');$('#AutoAnnotator_container_1381266044751 #hydrophobicity_charge_placeholder' + Math.floor((dis_datapoints[j][1] - 1)/200) ).append('<div class=\'AutoAnnotator_dis\' style=\'left:' + ((pos_of_first_tick - 8 + (dis_datapoints[j][1] - 1)*tick_diff - Math.floor((dis_datapoints[j][1] - 1)/200)*200*tick_diff).toFixed(0)).toString() + 'px;\'><b>' + (j+1) + '</b></div>');}}if($('#AutoAnnotator_container_1381266044751 #trans_checkbox').prop('checked') == true){for ( j = 0 ; j < trans_datapoints.length ; j++ ){$('#AutoAnnotator_container_1381266044751 #hydrophobicity_charge_placeholder' + Math.floor((trans_datapoints[j][0] - 1)/200) ).append('<div class=\'AutoAnnotator_trans\' style=\'width:' + (((trans_datapoints[j][1] - trans_datapoints[j][0] + 1)*tick_diff).toFixed(0)).toString() + 'px ;left:' + ((pos_of_first_tick + (trans_datapoints[j][0] - 1.5)*tick_diff - Math.floor((trans_datapoints[j][0] - 1)/200)*200*tick_diff).toFixed(0)).toString() + 'px\'></div>');}}if($('#AutoAnnotator_container_1381266044751 #sec_checkbox').prop('checked') == true){for ( j = 0 ; j < sec_helix_datapoints.length ; j++ ){$('#AutoAnnotator_container_1381266044751 #hydrophobicity_charge_placeholder' + Math.floor((sec_helix_datapoints[j][0] - 1)/200) ).append('<div class=\'AutoAnnotator_sec_helix\' style=\'width:' + (((sec_helix_datapoints[j][1] - sec_helix_datapoints[j][0] + 1)*tick_diff).toFixed(0)).toString() + 'px; left:' + ((pos_of_first_tick + (sec_helix_datapoints[j][0] - 1.5)*tick_diff - Math.floor((sec_helix_datapoints[j][0] - 1)/200)*200*tick_diff).toFixed(0)).toString() + 'px\'></div>');}for ( j = 0 ; j < sec_strand_datapoints.length ; j++ ){$('#AutoAnnotator_container_1381266044751 #hydrophobicity_charge_placeholder' + Math.floor((sec_strand_datapoints[j][0] - 1)/200) ).append('<div class=\'AutoAnnotator_sec_strand\' style=\'width:' + (((sec_strand_datapoints[j][1] - sec_strand_datapoints[j][0] + 1)*tick_diff).toFixed(0)).toString() + 'px; left:' + ((pos_of_first_tick + (sec_strand_datapoints[j][0] - 1.5)*tick_diff - Math.floor((sec_strand_datapoints[j][0] - 1)/200)*200*tick_diff).toFixed(0)).toString() + 'px\'></div>');}}if($('#AutoAnnotator_container_1381266044751 #acc_checkbox').prop('checked') == true){for ( j = 0 ; j < acc_buried_datapoints.length ; j++ ){$('#AutoAnnotator_container_1381266044751 #hydrophobicity_charge_placeholder' + Math.floor((acc_buried_datapoints[j][0] - 1)/200) ).append('<div class=\'AutoAnnotator_acc_buried\' style=\'width:' + (((acc_buried_datapoints[j][1] - acc_buried_datapoints[j][0] + 1)*tick_diff).toFixed(0)).toString() + 'px; left:' + ((pos_of_first_tick + (acc_buried_datapoints[j][0] - 1.5)*tick_diff - Math.floor((acc_buried_datapoints[j][0] - 1)/200)*200*tick_diff).toFixed(0)).toString() + 'px\'></div>');}for ( j = 0 ; j < acc_exposed_datapoints.length ; j++ ){$('#AutoAnnotator_container_1381266044751 #hydrophobicity_charge_placeholder' + Math.floor((acc_exposed_datapoints[j][0] - 1)/200) ).append('<div class=\'AutoAnnotator_acc_exposed\' style=\'width:' + (((acc_exposed_datapoints[j][1] - acc_exposed_datapoints[j][0] + 1)*tick_diff).toFixed(0)).toString() + 'px; left:' + ((pos_of_first_tick + (acc_exposed_datapoints[j][0] - 1.5)*tick_diff - Math.floor((acc_exposed_datapoints[j][0] - 1)/200)*200*tick_diff).toFixed(0)).toString() + 'px\'></div>');}}}catch(err){txt='There was an error while drawing the selected elements for the plot.\n';txt=txt+'The originating error is:\n' + err + '\n\n';throw(txt);}}</script></html> | |
<!-- Uncomment this to enable Functional Parameter display | <!-- Uncomment this to enable Functional Parameter display | ||
Latest revision as of 21:01, 8 October 2013
TMD of SERK Receptor from Physcomitrella patens with C-terminal GFP fusion in RFC[25]
This part codes for the Transmembrane Domain of SERK Receptor from Physcomitrella patens with a C-terminal (intracellular) GFPmut1 fusion; SERK TMD and GFPmut1 are seperated by a middle long linker. Still, a signal peptide is needed for membrane integration. This part is flanked by RFC[25] pre- and suffix for further protein fusions.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 863
| Protein data table for BioBrick BBa_ automatically created by the BioBrick-AutoAnnotator version 1.0 | ||||||||||||||||||||||||||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Nucleotide sequence in RFC 25, so ATGGCCGGC and ACCGGT were added (in italics) to the 5' and 3' ends: (underlined part encodes the protein) ATGGCCGGCCCTGGACAA ... CTCTACAAAACCGGT ORF from nucleotide position -8 to 939 (excluding stop-codon) | ||||||||||||||||||||||||||||||||||||||||||||||
Amino acid sequence: (RFC 25 scars in shown in bold, other sequence features underlined; both given below)
| ||||||||||||||||||||||||||||||||||||||||||||||
Sequence features: (with their position in the amino acid sequence, see the list of supported features)
| ||||||||||||||||||||||||||||||||||||||||||||||
Amino acid composition:
| ||||||||||||||||||||||||||||||||||||||||||||||
Amino acid counting
| Biochemical parameters
| |||||||||||||||||||||||||||||||||||||||||||||
| Plot for hydrophobicity, charge, predicted secondary structure, solvent accessability, transmembrane helices and disulfid bridges | ||||||||||||||||||||||||||||||||||||||||||||||
Codon usage
| ||||||||||||||||||||||||||||||||||||||||||||||
Alignments (obtained from PredictProtein.org)
| ||||||||||||||||||||||||||||||||||||||||||||||
| Predictions (obtained from PredictProtein.org) | ||||||||||||||||||||||||||||||||||||||||||||||
Subcellular Localization (reliability in brackets)
| Gene Ontology (reliability in brackets)
| |||||||||||||||||||||||||||||||||||||||||||||
Predicted features:
| ||||||||||||||||||||||||||||||||||||||||||||||
| The BioBrick-AutoAnnotator was created by TU-Munich 2013 iGEM team. For more information please see the documentation. If you have any questions, comments or suggestions, please leave us a comment. | ||||||||||||||||||||||||||||||||||||||||||||||