Difference between revisions of "Part:BBa K1159019"
m |
|||
| Line 10: | Line 10: | ||
<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
<partinfo>BBa_K1159019 SequenceAndFeatures</partinfo> | <partinfo>BBa_K1159019 SequenceAndFeatures</partinfo> | ||
| − | + | <html><!--- Please copy this table containing parameters for BBa_ at the end of the parametrs section ahead of the references. ---><style type="text/css">table#AutoAnnotator {border:1px solid black; width:100%; border-collapse:collapse;} th#AutoAnnotatorHeader { border:1px solid black; width:100%; background-color: rgb(221, 221, 221);} td.AutoAnnotator1col { width:100%; border:1px solid black; } span.AutoAnnotatorSequence { font-family:'Courier New', Arial; } td.AutoAnnotatorSeqNum { text-align:right; width:2%; } td.AutoAnnotatorSeqSeq { width:98% } td.AutoAnnotatorSeqFeat1 { width:3% } td.AutoAnnotatorSeqFeat2a { width:27% } td.AutoAnnotatorSeqFeat2b { width:97% } td.AutoAnnotatorSeqFeat3 { width:70% } table.AutoAnnotatorNoBorder { border:0px; width:100%; border-collapse:collapse; } table.AutoAnnotatorWithBorder { border:1px solid black; width:100%; border-collapse:collapse; } td.AutoAnnotatorOuterAmino { border:0px solid black; width:20% } td.AutoAnnotatorInnerAmino { border:1px solid black; width:50% } td.AutoAnnotatorAminoCountingOuter { border:1px solid black; width:40%; } td.AutoAnnotatorBiochemParOuter { border:1px solid black; width:60%; } td.AutoAnnotatorAminoCountingInner1 { width: 7.5% } td.AutoAnnotatorAminoCountingInner2 { width:62.5% } td.AutoAnnotatorAminoCountingInner3 { width:30% } td.AutoAnnotatorBiochemParInner1 { width: 5% } td.AutoAnnotatorBiochemParInner2 { width:55% } td.AutoAnnotatorBiochemParInner3 { width:40% } td.AutoAnnotatorCodonUsage1 { width: 3% } td.AutoAnnotatorCodonUsage2 { width:14.2% } td.AutoAnnotatorCodonUsage3 { width:13.8% } td.AutoAnnotatorAlignment1 { width: 3% } td.AutoAnnotatorAlignment2 { width: 10% } td.AutoAnnotatorAlignment3 { width: 87% } td.AutoAnnotatorLocalizationOuter {border:1px solid black; width:40%} td.AutoAnnotatorGOOuter {border:1px solid black; width:60%} td.AutoAnnotatorLocalization1 { width: 7.5% } td.AutoAnnotatorLocalization2 { width: 22.5% } td.AutoAnnotatorLocalization3 { width: 70% } td.AutoAnnotatorGO1 { width: 5% } td.AutoAnnotatorGO2 { width: 35% } td.AutoAnnotatorGO3 { width: 60% } td.AutoAnnotatorPredFeat1 { width:3% } td.AutoAnnotatorPredFeat2a { width:27% } td.AutoAnnotatorPredFeat3 { width:70% } div.AutoAnnotator_trans { position:absolute; background:rgb(11,140,143); background-color:rgba(11,140,143, 0.8); height:5px; top:100px; } div.AutoAnnotator_sec_helix { position:absolute; background:rgb(102,0,102); background-color:rgba(102,0,102, 0.8); height:5px; top:110px; } div.AutoAnnotator_sec_strand { position:absolute; background:rgb(245,170,26); background-color:rgba(245,170,26, 1); height:5px; top:110px; } div.AutoAnnotator_acc_buried { position:absolute; background:rgb(89,168,15); background-color:rgba(89,168,15, 0.8); height:5px; top:120px; } div.AutoAnnotator_acc_exposed { position:absolute; background:rgb(0, 0, 255); background-color:rgba(0, 0, 255, 0.8); height:5px; top:120px; } div.AutoAnnotator_dis { position:absolute; text-align:center; font-family:Arial,Helvetica,sans-serif; background:rgb(255, 200, 0); background-color:rgba(255, 200, 0, 1); height:16px; width:16px; top:80px; border-radius:50%; } </style><div id='AutoAnnotator_container_1381244609553'><table id="AutoAnnotator"><tr><!-- Time stamp in ms since 1/1/1970 1381244609553 --><th id="AutoAnnotatorHeader" colspan="2">Protein data table for BioBrick <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_<!------------------------Enter BioBrick number here------------------------>">BBa_<!------------------------Enter BioBrick number here------------------------></a> automatically created by the <a href="http://2013.igem.org/Team:TU-Munich/Results/AutoAnnotator">BioBrick-AutoAnnotator</a> version 1.0</th></tr><tr><td class="AutoAnnotator1col" colspan="2"><strong>Nucleotide sequence</strong> in <strong>RFC 25 N-Part</strong> using the stop codon in the suffix, so ACCGGT was added (in italics) to the 3' end: (underlined part encodes the protein)<br><span class="AutoAnnotatorSequence"> AACA<u>ATGCCTGGT ... CTCTACAAA<i>ACCGGT</i></u></span><br> <strong>ORF</strong> from nucleotide position 5 to 2086 (excluding stop-codon)</td></tr><tr><td class="AutoAnnotator1col" colspan="2"><strong>Amino acid sequence:</strong> (RFC 25 scars in shown in bold, other sequence features underlined; both given below)<br><span class="AutoAnnotatorSequence"><table class="AutoAnnotatorNoBorder"><tr><td class="AutoAnnotatorSeqNum">1 <br>101 <br>201 <br>301 <br>401 <br>501 <br>601 </td><td class="AutoAnnotatorSeqSeq">MPGEVAWWRPLFLIALMPIGVLSNAEGDALNT<b>TG</b>GSADLDKLNIDSIIQRLLEVRGSKPGKNVQLQENEIRGLCLKSREIFLSQPILLELEAPLKICGDI<br>HGQYYDLLRLFEYGGFPPESNYLFLGDYVDRGKQSLETICLLLAYKIKYPENFFLLRGNHECASINRIYGFYDECKRRYNIKLWKTFTDCFNCLPIAAIV<br>DEKIFCCHGGLSPDLQSMEQIRRIMRPTDVPDQGLLCDLLWSDPDKDVLGWGENDRGVSFTFGAEVVAKFLHKHDLDLICRAHQVVEDGYEFFAKRQLVT<br>LFSAPNYCGEFDNAGAMMSVDETLMCSFQILKPAEKKKPNATRPVTPPRGMITKQAKK<b>TG</b>SA<u>WSHPQFEK</u>G<u>ENLYFQS</u>G<b>TG</b>PGQPPFPPPPPFTPPPPQT<br>PNGASGENSTGAIAGGVAAGAALLFAAPAIGFAWWRRRRPIEA<b>TG</b>GGSGGGSG<b>TG</b>SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF<br>ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYN<br>SHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLSPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITHGMDELYKTG*</td></tr></table></span></td></tr><tr><td class="AutoAnnotator1col" colspan="2"><strong>Sequence features:</strong> (with their position in the amino acid sequence, see the <a href="http://2013.igem.org/Team:TU-Munich/Results/Software/FeatureList">list of supported features</a>)<table class="AutoAnnotatorNoBorder"><tr><td class="AutoAnnotatorSeqFeat1"></td><td class="AutoAnnotatorSeqFeat2a">RFC25 scar (shown in bold): </td><td class="AutoAnnotatorSeqFeat3">33 to 34, 359 to 360, 380 to 381, 444 to 445, 454 to 455</td></tr><tr><td class="AutoAnnotatorSeqFeat1"></td><td class="AutoAnnotatorSeqFeat2a"><i>Strep</i>-tag II: </td><td class="AutoAnnotatorSeqFeat3">363 to 370</td></tr><tr><td class="AutoAnnotatorSeqFeat1"></td><td class="AutoAnnotatorSeqFeat2a">TEV cleavage site: </td><td class="AutoAnnotatorSeqFeat3">372 to 378</td></tr></table></td></tr><tr><td class="AutoAnnotator1col" colspan="2"><strong>Amino acid composition:</strong><table class="AutoAnnotatorNoBorder"><tr><td class="AutoAnnotatorOuterAmino"><table class="AutoAnnotatorWithBorder"><tr><td class="AutoAnnotatorInnerAmino">Ala (A)</td><td class="AutoAnnotatorInnerAmino">41 (5.9%)</td></tr><tr><td class="AutoAnnotatorInnerAmino">Arg (R)</td><td class="AutoAnnotatorInnerAmino">29 (4.2%)</td></tr><tr><td class="AutoAnnotatorInnerAmino">Asn (N)</td><td class="AutoAnnotatorInnerAmino">31 (4.5%)</td></tr><tr><td class="AutoAnnotatorInnerAmino">Asp (D)</td><td class="AutoAnnotatorInnerAmino">42 (6.1%)</td></tr></table></td><td class="AutoAnnotatorOuterAmino"><table class="AutoAnnotatorWithBorder"><tr><td class="AutoAnnotatorInnerAmino">Cys (C)</td><td class="AutoAnnotatorInnerAmino">15 (2.2%)</td></tr><tr><td class="AutoAnnotatorInnerAmino">Gln (Q)</td><td class="AutoAnnotatorInnerAmino">25 (3.6%)</td></tr><tr><td class="AutoAnnotatorInnerAmino">Glu (E)</td><td class="AutoAnnotatorInnerAmino">43 (6.2%)</td></tr><tr><td class="AutoAnnotatorInnerAmino">Gly (G)</td><td class="AutoAnnotatorInnerAmino">70 (10.1%)</td></tr></table></td><td class="AutoAnnotatorOuterAmino"><table class="AutoAnnotatorWithBorder"><tr><td class="AutoAnnotatorInnerAmino">His (H)</td><td class="AutoAnnotatorInnerAmino">17 (2.4%)</td></tr><tr><td class="AutoAnnotatorInnerAmino">Ile (I)</td><td class="AutoAnnotatorInnerAmino">38 (5.5%)</td></tr><tr><td class="AutoAnnotatorInnerAmino">Leu (L)</td><td class="AutoAnnotatorInnerAmino">66 (9.5%)</td></tr><tr><td class="AutoAnnotatorInnerAmino">Lys (K)</td><td class="AutoAnnotatorInnerAmino">44 (6.3%)</td></tr></table></td><td class="AutoAnnotatorOuterAmino"><table class="AutoAnnotatorWithBorder"><tr><td class="AutoAnnotatorInnerAmino">Met (M)</td><td class="AutoAnnotatorInnerAmino">13 (1.9%)</td></tr><tr><td class="AutoAnnotatorInnerAmino">Phe (F)</td><td class="AutoAnnotatorInnerAmino">37 (5.3%)</td></tr><tr><td class="AutoAnnotatorInnerAmino">Pro (P)</td><td class="AutoAnnotatorInnerAmino">46 (6.6%)</td></tr><tr><td class="AutoAnnotatorInnerAmino">Ser (S)</td><td class="AutoAnnotatorInnerAmino">34 (4.9%)</td></tr></table></td><td class="AutoAnnotatorOuterAmino"><table class="AutoAnnotatorWithBorder"><tr><td class="AutoAnnotatorInnerAmino">Thr (T)</td><td class="AutoAnnotatorInnerAmino">36 (5.2%)</td></tr><tr><td class="AutoAnnotatorInnerAmino">Trp (W)</td><td class="AutoAnnotatorInnerAmino">9 (1.3%)</td></tr><tr><td class="AutoAnnotatorInnerAmino">Tyr (Y)</td><td class="AutoAnnotatorInnerAmino">24 (3.5%)</td></tr><tr><td class="AutoAnnotatorInnerAmino">Val (V)</td><td class="AutoAnnotatorInnerAmino">34 (4.9%)</td></tr></table></td></tr></table></td></tr><tr><td class="AutoAnnotatorAminoCountingOuter"><strong>Amino acid counting</strong><table class="AutoAnnotatorNoBorder"><tr><td class="AutoAnnotatorAminoCountingInner1"></td><td class="AutoAnnotatorAminoCountingInner2">Total number:</td><td class="AutoAnnotatorAminoCountingInner3">694</td></tr><tr><td class="AutoAnnotatorAminoCountingInner1"></td><td class="AutoAnnotatorAminoCountingInner2">Positively charged (Arg+Lys):</td><td class="AutoAnnotatorAminoCountingInner3">73 (10.5%)</td></tr><tr><td class="AutoAnnotatorAminoCountingInner1"></td><td class="AutoAnnotatorAminoCountingInner2">Negatively charged (Asp+Glu):</td><td class="AutoAnnotatorAminoCountingInner3">85 (12.2%)</td></tr><tr><td class="AutoAnnotatorAminoCountingInner1"></td><td class="AutoAnnotatorAminoCountingInner2">Aromatic (Phe+His+Try+Tyr):</td><td class="AutoAnnotatorAminoCountingInner3">87 (12.5%)</td></tr></table></td><td class="AutoAnnotatorBiochemParOuter"><strong>Biochemical parameters</strong><table class="AutoAnnotatorNoBorder"><tr><td class="AutoAnnotatorBiochemParInner1"></td><td class="AutoAnnotatorBiochemParInner2">Atomic composition:</td><td class="AutoAnnotatorBiochemParInner3">C<sub>3463</sub>H<sub>5334</sub>N<sub>924</sub>O<sub>1015</sub>S<sub>28</sub></td></tr><tr><td class="AutoAnnotatorBiochemParInner1"></td><td class="AutoAnnotatorBiochemParInner2">Molecular mass [Da]:</td><td class="AutoAnnotatorBiochemParInner3">77049.7</td></tr><tr><td class="AutoAnnotatorBiochemParInner1"></td><td class="AutoAnnotatorBiochemParInner2">Theoretical pI:</td><td class="AutoAnnotatorBiochemParInner3">5.85</td></tr><tr><td class="AutoAnnotatorBiochemParInner1"></td><td class="AutoAnnotatorBiochemParInner2">Extinction coefficient at 280 nm [M<sup>-1</sup> cm<sup>-1</sup>]:</td><td class="AutoAnnotatorBiochemParInner3">85260 / 86198 (all Cys red/ox)</td></tr></table></td></tr><tr><td class="AutoAnnotator1col" colspan="2"><strong>Plot for hydrophobicity, charge, predicted secondary structure, solvent accessability, transmembrane helices and disulfid bridges</strong> <input type='button' id='hydrophobicity_charge_button' onclick='show_or_hide_plot_1381244609553()' value='Show'><span id="hydrophobicity_charge_explanation"></span><div id="hydrophobicity_charge_container" style='display:none'><div id="hydrophobicity_charge_placeholder0" style="width:100%;height:150px"></div><div id="hydrophobicity_charge_placeholder1" style="width:100%;height:150px"></div><div id="hydrophobicity_charge_placeholder2" style="width:100%;height:150px"></div><div id="hydrophobicity_charge_placeholder3" style="width:100%;height:150px"></div></div></td></tr><tr><td class="AutoAnnotator1col" colspan="2"><strong>Codon usage</strong><table class="AutoAnnotatorNoBorder"><tr><td class="AutoAnnotatorCodonUsage1"></td><td class="AutoAnnotatorCodonUsage2">Organism:</td><td class="AutoAnnotatorCodonUsage3"><i>E. coli</i></td><td class="AutoAnnotatorCodonUsage3"><i>B. subtilis</i></td><td class="AutoAnnotatorCodonUsage3"><i>S. cerevisiae</i></td><td class="AutoAnnotatorCodonUsage3"><i>A. thaliana</i></td><td class="AutoAnnotatorCodonUsage3"><i>P. patens</i></td><td class="AutoAnnotatorCodonUsage3">Mammals</td></tr><tr><td class="AutoAnnotatorCodonUsage1"></td><td class="AutoAnnotatorCodonUsage2">Codon quality (<a href="http://en.wikipedia.org/wiki/Codon_Adaptation_Index">CAI</a>):</td><td class="AutoAnnotatorCodonUsage3">good (0.71)</td><td class="AutoAnnotatorCodonUsage3">good (0.75)</td><td class="AutoAnnotatorCodonUsage3">good (0.72)</td><td class="AutoAnnotatorCodonUsage3">excellent (0.81)</td><td class="AutoAnnotatorCodonUsage3">excellent (0.80)</td><td class="AutoAnnotatorCodonUsage3">good (0.71)</td></tr></table></td></tr><tr><td class="AutoAnnotator1col" colspan="2"><strong>Alignments</strong> (obtained from <a href='http://predictprotein.org'>PredictProtein.org</a>)<table class="AutoAnnotatorNoBorder"><tr><td class="AutoAnnotatorAlignment1"></td><td class="AutoAnnotatorAlignment2">SwissProt:</td><td class="AutoAnnotatorAlignment3"><a href='http://www.uniprot.org/uniprot/P36873'>P36873</a> (100% identity on 322 AAs), <a href='http://www.uniprot.org/uniprot/P36874'>P36874</a> (99% identity on 322 AAs), <a href='http://www.uniprot.org/uniprot/P61287'>P61287</a> (99% identity on 322 AAs), <a href='http://www.uniprot.org/uniprot/P63087'>P63087</a> (99% identity on 322 AAs), <a href='http://www.uniprot.org/uniprot/P63088'>P63088</a> (99% identity on 322 AAs), <a href='http://www.uniprot.org/uniprot/Q6NVU2'>Q6NVU2</a> (99% identity on 322 AAs), <a href='http://www.uniprot.org/uniprot/Q7SZ10'>Q7SZ10</a> (99% identity on 322 AAs), <a href='http://www.uniprot.org/uniprot/P42212'>P42212</a> (98% identity on 238 AAs), <a href='http://www.uniprot.org/uniprot/Q8MJ46'>Q8MJ46</a> (98% identity on 322 AAs)</td></tr><tr><td class="AutoAnnotatorAlignment1"></td><td class="AutoAnnotatorAlignment2">TrEML:</td><td class="AutoAnnotatorAlignment3"><a href='http://www.uniprot.org/uniprot/F6YKN7'>F6YKN7</a> (100% identity on 322 AAs), <a href='http://www.uniprot.org/uniprot/F7AYW3'>F7AYW3</a> (100% identity on 305 AAs), <a href='http://www.uniprot.org/uniprot/F7GFU1'>F7GFU1</a> (100% identity on 322 AAs), <a href='http://www.uniprot.org/uniprot/G5AWL7'>G5AWL7</a> (100% identity on 296 AAs), <a href='http://www.uniprot.org/uniprot/H9FWE1'>H9FWE1</a> (100% identity on 321 AAs), <a href='http://www.uniprot.org/uniprot/K7A7K5'>K7A7K5</a> (100% identity on 322 AAs), <a href='http://www.uniprot.org/uniprot/Q4R4V0'>Q4R4V0</a> (100% identity on 322 AAs), <a href='http://www.uniprot.org/uniprot/Q9UPN1'>Q9UPN1</a> (100% identity on 293 AAs), <a href='http://www.uniprot.org/uniprot/B5KFI8'>B5KFI8</a> (99% identity on 322 AAs), <a href='http://www.uniprot.org/uniprot/F8VYE8'>F8VYE8</a> (99% identity on 294 AAs)</td></tr><tr><td class="AutoAnnotatorAlignment1"></td><td class="AutoAnnotatorAlignment2">PDB:</td><td class="AutoAnnotatorAlignment3"><a href='http://www.rcsb.org/pdb/explore/explore.do?structureId=1it6'>1it6</a> (100% identity on 293 AAs), <a href='http://www.rcsb.org/pdb/explore/explore.do?structureId=1jk7'>1jk7</a> (100% identity on 294 AAs), <a href='http://www.rcsb.org/pdb/explore/explore.do?structureId=2bcd'>2bcd</a> (100% identity on 293 AAs), <a href='http://www.rcsb.org/pdb/explore/explore.do?structureId=2bdx'>2bdx</a> (100% identity on 293 AAs), <a href='http://www.rcsb.org/pdb/explore/explore.do?structureId=2o8a'>2o8a</a> (100% identity on 295 AAs), <a href='http://www.rcsb.org/pdb/explore/explore.do?structureId=2o8g'>2o8g</a> (100% identity on 295 AAs), <a href='http://www.rcsb.org/pdb/explore/explore.do?structureId=1u32'>1u32</a> (98% identity on 293 AAs)</td></tr></table></td></tr><tr><th id='AutoAnnotatorHeader' colspan="2"><strong>Predictions</strong> (obtained from <a href='http://predictprotein.org'>PredictProtein.org</a>)</th></tr><tr><td class="AutoAnnotatorLocalizationOuter"><strong>Subcellular Localization</strong> (reliability in brackets)<table class="AutoAnnotatorNoBorder"><tr><td class="AutoAnnotatorLocalization1"></td><td class="AutoAnnotatorLocalization2">Archaea:</td><td class="AutoAnnotatorLocalization3">cytosol (100%)</td></tr><tr><td class="AutoAnnotatorLocalization1"></td><td class="AutoAnnotatorLocalization2">Bacteria:</td><td class="AutoAnnotatorLocalization3">cytosol (59%)</td></tr><tr><td class="AutoAnnotatorLocalization1"></td><td class="AutoAnnotatorLocalization2">Eukarya:</td><td class="AutoAnnotatorLocalization3">cytosol (40%)</td></tr></table></td><td class="AutoAnnotatorGOOuter"><strong>Gene Ontology</strong> (reliability in brackets)<br><table class="AutoAnnotatorNoBorder"><tr><td class='AutoAnnotatorGO1'></td><td class='AutoAnnotatorGO2'>Molecular Function Ontology:</td><td class='AutoAnnotatorGO3'><a href='http://amigo.geneontology.org/cgi-bin/amigo/term_details?term=GO:0005515'>GO:0005515</a> (70%), <a href='http://amigo.geneontology.org/cgi-bin/amigo/term_details?term=GO:0004722'>GO:0004722</a> (70%)</td></tr><tr><td class='AutoAnnotatorGO1'></td><td class='AutoAnnotatorGO2'>Biological Process Ontology:</td><td class='AutoAnnotatorGO3'><a href='http://amigo.geneontology.org/cgi-bin/amigo/term_details?term=GO:0051301'>GO:0051301</a> (28%), <a href='http://amigo.geneontology.org/cgi-bin/amigo/term_details?term=GO:0005977'>GO:0005977</a> (27%)</td></tr><tr><td class='AutoAnnotatorGO1'> </td><td class='AutoAnnotatorGO2'> </td><td class='AutoAnnotatorGO3'> </td></tr></table></td></tr><tr><td class="AutoAnnotator1col" colspan="2"><strong>Predicted features:</strong><table class="AutoAnnotatorNoBorder"><tr><td class="AutoAnnotatorPredFeat1"></td><td class="AutoAnnotatorPredFeat2a">Disulfid bridges:</td><td class="AutoAnnotatorPredFeat3"> - </td></tr><tr><td class="AutoAnnotatorPredFeat1"></td><td class="AutoAnnotatorPredFeat2a">Transmembrane helices:</td><td class="AutoAnnotatorPredFeat3"> - </td></tr></table></td></tr><tr><td class="AutoAnnotator1col" colspan="2"> The BioBrick-AutoAnnotator was created by <a href="http://2013.igem.org/Team:TU-Munich">TU-Munich 2013</a> iGEM team. For more information please see the <a href="http://2013.igem.org/Team:TU-Munich/Results/Software">documentation</a>.<br>If you have any questions, comments or suggestions, please leave us a <a href="http://2013.igem.org/Team:TU-Munich/Results/AutoAnnotator">comment</a>.</td></tr></table></div><br><!-- IMPORTANT: DON'T REMOVE THIS LINE, OTHERWISE NOT SUPPORTED FOR IE BEFORE 9 --><!--[if lte IE 8]><script language="javascript" type="text/javascript" src="excanvas.min.js"></script><![endif]--><script type='text/javascript' src='http://code.jquery.com/jquery-1.10.0.min.js'></script><script type='text/javascript' src='http://2013.igem.org/Team:TU-Munich/Flot.js?action=raw&ctype=text/js'></script><script>function show_or_hide_plot_1381244609553(){hydrophobicity_datapoints = [[2.5,0.12],[3.5,0.10],[4.5,0.24],[5.5,0.14],[6.5,-0.06],[7.5,-1.22],[8.5,-0.82],[9.5,-0.08],[10.5,0.86],[11.5,2.66],[12.5,3.34],[13.5,3.34],[14.5,3.16],[15.5,2.08],[16.5,2.08],[17.5,1.64],[18.5,1.72],[19.5,2.10],[20.5,2.26],[21.5,0.66],[22.5,1.10],[23.5,-0.44],[24.5,-1.28],[25.5,-1.82],[26.5,-0.76],[27.5,-0.36],[28.5,-0.36],[29.5,-0.42],[30.5,0.14],[31.5,-0.30],[32.5,-1.14],[33.5,-0.60],[34.5,-0.10],[35.5,-0.66],[36.5,0.18],[37.5,-0.44],[38.5,-1.06],[39.5,-0.66],[40.5,-0.66],[41.5,-0.52],[42.5,-0.52],[43.5,0.10],[44.5,0.24],[45.5,1.84],[46.5,0.24],[47.5,0.04],[48.5,0.96],[49.5,0.82],[50.5,-0.78],[51.5,0.76],[52.5,0.76],[53.5,-0.08],[54.5,-1.00],[55.5,-1.08],[56.5,-2.24],[57.5,-1.42],[58.5,-2.12],[59.5,-2.66],[60.5,-1.04],[61.5,-1.42],[62.5,-0.58],[63.5,-0.50],[64.5,-0.50],[65.5,-2.04],[66.5,-2.04],[67.5,-1.90],[68.5,-2.10],[69.5,-1.48],[70.5,-0.02],[71.5,1.18],[72.5,1.04],[73.5,1.16],[74.5,1.08],[75.5,-0.58],[76.5,-1.78],[77.5,-1.64],[78.5,-0.30],[79.5,0.62],[80.5,1.36],[81.5,1.36],[82.5,0.14],[83.5,0.48],[84.5,0.48],[85.5,1.40],[86.5,1.40],[87.5,2.48],[88.5,0.88],[89.5,0.48],[90.5,-0.60],[91.5,0.86],[92.5,-0.68],[93.5,0.92],[94.5,1.06],[95.5,1.30],[96.5,-0.16],[97.5,1.52],[98.5,-0.02],[99.5,-0.60],[100.5,-1.22],[101.5,-0.78],[102.5,-1.94],[103.5,-2.00],[104.5,-1.16],[105.5,0.30],[106.5,-0.34],[107.5,0.68],[108.5,1.94],[109.5,0.48],[110.5,-0.54],[111.5,0.28],[112.5,-0.56],[113.5,-0.56],[114.5,-0.18],[115.5,-0.24],[116.5,-0.86],[117.5,-0.94],[118.5,-2.20],[119.5,-2.14],[120.5,-1.06],[121.5,0.20],[122.5,1.12],[123.5,1.74],[124.5,1.30],[125.5,0.28],[126.5,0.56],[127.5,-0.90],[128.5,-1.72],[129.5,-1.10],[130.5,-1.62],[131.5,-3.16],[132.5,-2.62],[133.5,-0.96],[134.5,-1.58],[135.5,-0.94],[136.5,0.66],[137.5,1.32],[138.5,1.32],[139.5,2.78],[140.5,3.68],[141.5,3.14],[142.5,2.38],[143.5,0.84],[144.5,0.98],[145.5,-0.56],[146.5,-1.18],[147.5,-1.24],[148.5,-1.16],[149.5,-2.76],[150.5,-1.42],[151.5,-0.60],[152.5,0.48],[153.5,1.94],[154.5,1.74],[155.5,1.10],[156.5,-0.16],[157.5,-1.56],[158.5,-3.02],[159.5,-1.62],[160.5,-1.18],[161.5,-0.64],[162.5,0.90],[163.5,0.90],[164.5,-0.50],[165.5,0.04],[166.5,-0.06],[167.5,-1.04],[168.5,0.22],[169.5,0.86],[170.5,-0.74],[171.5,-1.18],[172.5,-0.60],[173.5,-1.94],[174.5,-2.58],[175.5,-2.78],[176.5,-2.34],[177.5,-3.54],[178.5,-1.86],[179.5,-1.74],[180.5,-0.08],[181.5,-0.00],[182.5,-0.08],[183.5,-1.12],[184.5,0.22],[185.5,-0.68],[186.5,-1.20],[187.5,0.08],[188.5,0.78],[189.5,-0.48],[190.5,0.16],[191.5,1.62],[192.5,0.80],[193.5,1.14],[194.5,2.20],[195.5,2.06],[196.5,2.20],[197.5,3.36],[198.5,1.76],[199.5,0.70],[200.5,-0.44],[201.5,-0.44],[202.5,-0.72],[203.5,0.48],[204.5,1.68],[205.5,1.82],[206.5,0.84],[207.5,0.20],[208.5,0.46],[209.5,-0.20],[210.5,0.12],[211.5,-0.50],[212.5,0.34],[213.5,-1.12],[214.5,-1.12],[215.5,-0.42],[216.5,-0.42],[217.5,-1.88],[218.5,-0.28],[219.5,-1.02],[220.5,-2.30],[221.5,-0.70],[222.5,0.38],[223.5,-1.42],[224.5,-0.84],[225.5,-0.08],[226.5,-1.68],[227.5,-1.22],[228.5,-0.64],[229.5,-1.02],[230.5,-1.58],[231.5,-0.96],[232.5,-1.04],[233.5,0.04],[234.5,1.24],[235.5,1.24],[236.5,2.08],[237.5,2.08],[238.5,1.14],[239.5,0.48],[240.5,0.48],[241.5,-0.60],[242.5,-2.06],[243.5,-2.66],[244.5,-3.20],[245.5,-1.66],[246.5,-0.58],[247.5,0.04],[248.5,0.64],[249.5,1.26],[250.5,-0.28],[251.5,-1.74],[252.5,-2.36],[253.5,-3.08],[254.5,-3.08],[255.5,-1.54],[256.5,-1.00],[257.5,0.26],[258.5,1.02],[259.5,1.66],[260.5,0.74],[261.5,1.26],[262.5,-0.00],[263.5,0.98],[264.5,1.26],[265.5,1.70],[266.5,0.56],[267.5,1.82],[268.5,1.74],[269.5,0.26],[270.5,-0.88],[271.5,-0.74],[272.5,-2.00],[273.5,-2.00],[274.5,-2.06],[275.5,-0.52],[276.5,1.02],[277.5,2.22],[278.5,0.56],[279.5,1.62],[280.5,0.22],[281.5,-1.38],[282.5,-1.04],[283.5,0.70],[284.5,-0.36],[285.5,-0.42],[286.5,0.20],[287.5,-0.90],[288.5,-2.44],[289.5,-1.18],[290.5,0.08],[291.5,0.52],[292.5,-0.00],[293.5,-0.20],[294.5,-1.46],[295.5,-1.26],[296.5,-0.78],[297.5,-0.14],[298.5,1.52],[299.5,2.78],[300.5,1.86],[301.5,1.38],[302.5,1.20],[303.5,-0.26],[304.5,-1.08],[305.5,-0.42],[306.5,-0.86],[307.5,-1.24],[308.5,0.02],[309.5,-0.42],[310.5,-1.62],[311.5,-1.18],[312.5,-0.56],[313.5,-0.76],[314.5,0.32],[315.5,1.40],[316.5,0.88],[317.5,1.80],[318.5,0.74],[319.5,-0.34],[320.5,-0.86],[321.5,0.06],[322.5,-0.40],[323.5,0.80],[324.5,1.34],[325.5,2.04],[326.5,0.58],[327.5,1.10],[328.5,1.36],[329.5,0.74],[330.5,-0.14],[331.5,0.92],[332.5,-0.68],[333.5,-2.22],[334.5,-2.22],[335.5,-2.68],[336.5,-3.36],[337.5,-3.36],[338.5,-2.22],[339.5,-1.58],[340.5,-1.70],[341.5,-1.70],[342.5,-0.16],[343.5,-0.66],[344.5,-0.84],[345.5,-0.26],[346.5,-0.84],[347.5,-1.76],[348.5,-1.24],[349.5,-0.02],[350.5,0.16],[351.5,0.28],[352.5,-0.34],[353.5,-0.36],[354.5,-2.04],[355.5,-2.68],[356.5,-2.04],[357.5,-1.42],[358.5,-1.94],[359.5,-0.80],[360.5,-0.20],[361.5,-0.22],[362.5,-0.78],[363.5,-0.94],[364.5,-2.00],[365.5,-1.26],[366.5,-1.80],[367.5,-1.94],[368.5,-1.70],[369.5,-1.70],[370.5,-2.96],[371.5,-1.50],[372.5,-0.98],[373.5,-0.34],[374.5,-0.34],[375.5,0.20],[376.5,-0.64],[377.5,-0.52],[378.5,-1.16],[379.5,-0.78],[380.5,-0.70],[381.5,-1.32],[382.5,-1.50],[383.5,-1.74],[384.5,-0.86],[385.5,-1.10],[386.5,-0.72],[387.5,-0.72],[388.5,-0.72],[389.5,-1.60],[390.5,-0.72],[391.5,-0.54],[392.5,-0.54],[393.5,-0.54],[394.5,-0.54],[395.5,-1.42],[396.5,-1.98],[397.5,-1.80],[398.5,-1.80],[399.5,-2.18],[400.5,-1.94],[401.5,-0.88],[402.5,-0.90],[403.5,-0.66],[404.5,-0.66],[405.5,-1.28],[406.5,-1.80],[407.5,-1.78],[408.5,-1.78],[409.5,-0.72],[410.5,0.88],[411.5,1.40],[412.5,1.46],[413.5,1.46],[414.5,1.94],[415.5,1.40],[416.5,1.40],[417.5,1.40],[418.5,1.84],[419.5,1.36],[420.5,1.76],[421.5,2.16],[422.5,2.80],[423.5,2.80],[424.5,2.80],[425.5,1.72],[426.5,1.32],[427.5,1.66],[428.5,1.22],[429.5,1.42],[430.5,2.10],[431.5,1.56],[432.5,0.48],[433.5,-0.34],[434.5,-1.80],[435.5,-3.06],[436.5,-3.78],[437.5,-3.92],[438.5,-2.12],[439.5,-1.92],[440.5,-0.66],[441.5,0.10],[442.5,0.34],[443.5,-0.64],[444.5,-0.02],[445.5,-0.54],[446.5,-0.48],[447.5,-0.48],[448.5,-0.48],[449.5,-0.56],[450.5,-0.48],[451.5,-0.54],[452.5,-0.54],[453.5,-0.62],[454.5,-1.24],[455.5,-1.24],[456.5,-1.80],[457.5,-2.42],[458.5,-1.50],[459.5,-0.16],[460.5,-0.22],[461.5,0.40],[462.5,1.94],[463.5,2.02],[464.5,1.14],[465.5,2.18],[466.5,3.02],[467.5,3.02],[468.5,1.48],[469.5,2.56],[470.5,0.96],[471.5,0.12],[472.5,-1.42],[473.5,0.12],[474.5,-1.34],[475.5,-0.72],[476.5,-1.28],[477.5,-1.36],[478.5,-1.64],[479.5,-1.10],[480.5,-0.18],[481.5,0.30],[482.5,1.00],[483.5,-0.26],[484.5,-0.18],[485.5,-1.72],[486.5,-1.64],[487.5,-2.26],[488.5,-1.20],[489.5,-1.26],[490.5,-0.82],[491.5,-0.82],[492.5,-0.90],[493.5,-0.50],[494.5,-0.50],[495.5,0.52],[496.5,-0.18],[497.5,1.16],[498.5,1.30],[499.5,1.94],[500.5,1.04],[501.5,1.68],[502.5,1.04],[503.5,-0.64],[504.5,-0.38],[505.5,-0.56],[506.5,0.42],[507.5,0.18],[508.5,0.78],[509.5,-0.30],[510.5,-0.12],[511.5,-0.20],[512.5,0.96],[513.5,1.00],[514.5,1.18],[515.5,2.08],[516.5,1.18],[517.5,0.08],[518.5,0.14],[519.5,1.12],[520.5,-0.34],[521.5,0.30],[522.5,1.12],[523.5,1.04],[524.5,-0.70],[525.5,-0.26],[526.5,-1.08],[527.5,-2.34],[528.5,-2.82],[529.5,-1.54],[530.5,-2.06],[531.5,-2.44],[532.5,-2.38],[533.5,-2.44],[534.5,-2.26],[535.5,-0.92],[536.5,-1.00],[537.5,-0.52],[538.5,0.54],[539.5,0.36],[540.5,-0.52],[541.5,-0.44],[542.5,-0.36],[543.5,-0.98],[544.5,-0.52],[545.5,-0.90],[546.5,-0.90],[547.5,-1.72],[548.5,-1.60],[549.5,-1.54],[550.5,-0.28],[551.5,0.98],[552.5,1.10],[553.5,0.54],[554.5,-1.06],[555.5,-1.70],[556.5,-2.96],[557.5,-2.44],[558.5,-2.52],[559.5,-1.96],[560.5,-2.78],[561.5,-1.72],[562.5,-2.16],[563.5,-0.54],[564.5,-1.18],[565.5,0.28],[566.5,-0.78],[567.5,-0.16],[568.5,-1.70],[569.5,-1.06],[570.5,-0.86],[571.5,0.68],[572.5,0.06],[573.5,-0.14],[574.5,0.90],[575.5,-0.56],[576.5,-0.64],[577.5,-0.72],[578.5,0.10],[579.5,0.10],[580.5,0.10],[581.5,-0.10],[582.5,-0.10],[583.5,-0.72],[584.5,-2.32],[585.5,-1.70],[586.5,-2.96],[587.5,-1.28],[588.5,0.18],[589.5,0.80],[590.5,0.24],[591.5,0.16],[592.5,0.02],[593.5,-1.44],[594.5,-1.62],[595.5,-1.68],[596.5,-1.16],[597.5,-2.62],[598.5,-2.08],[599.5,-2.46],[600.5,-2.46],[601.5,-1.36],[602.5,-0.92],[603.5,0.14],[604.5,1.16],[605.5,2.22],[606.5,0.68],[607.5,0.16],[608.5,-1.44],[609.5,-2.60],[610.5,-3.66],[611.5,-3.04],[612.5,-1.36],[613.5,-1.44],[614.5,0.18],[615.5,0.18],[616.5,0.82],[617.5,-0.86],[618.5,0.82],[619.5,-0.92],[620.5,-0.86],[621.5,-2.12],[622.5,-0.44],[623.5,-2.04],[624.5,-1.84],[625.5,-1.28],[626.5,-0.74],[627.5,-0.80],[628.5,-0.80],[629.5,0.66],[630.5,1.10],[631.5,0.56],[632.5,-0.92],[633.5,-0.48],[634.5,-1.94],[635.5,-3.00],[636.5,-3.00],[637.5,-2.50],[638.5,-2.56],[639.5,-0.96],[640.5,-0.34],[641.5,-0.34],[642.5,-0.28],[643.5,-0.28],[644.5,-0.34],[645.5,0.50],[646.5,1.04],[647.5,0.80],[648.5,0.42],[649.5,-1.12],[650.5,-2.52],[651.5,-2.62],[652.5,-1.54],[653.5,-1.00],[654.5,-0.44],[655.5,-0.50],[656.5,-0.40],[657.5,-0.80],[658.5,0.12],[659.5,0.10],[660.5,0.02],[661.5,-0.52],[662.5,-1.20],[663.5,-2.66],[664.5,-3.20],[665.5,-3.20],[666.5,-3.40],[667.5,-3.78],[668.5,-3.72],[669.5,-2.64],[670.5,-1.02],[671.5,0.64],[672.5,2.10],[673.5,2.04],[674.5,2.22],[675.5,2.22],[676.5,1.32],[677.5,0.92],[678.5,1.98],[679.5,1.34],[680.5,1.40],[681.5,1.40],[682.5,0.40],[683.5,-0.04],[684.5,0.42],[685.5,-1.18],[686.5,-1.74],[687.5,-0.34],[688.5,-0.52],[689.5,-1.68],[690.5,-1.12],[691.5,-0.50]];charge_datapoints = [[2.5,-0.20],[3.5,-0.20],[4.5,-0.20],[5.5,-0.20],[6.5,0.20],[7.5,0.20],[8.5,0.20],[9.5,0.20],[10.5,0.20],[11.5,0.00],[12.5,0.00],[13.5,0.00],[14.5,0.00],[15.5,0.00],[16.5,0.00],[17.5,0.00],[18.5,0.00],[19.5,0.00],[20.5,0.00],[21.5,0.00],[22.5,0.00],[23.5,-0.20],[24.5,-0.20],[25.5,-0.40],[26.5,-0.40],[27.5,-0.40],[28.5,-0.20],[29.5,-0.20],[30.5,0.00],[31.5,0.00],[32.5,0.00],[33.5,0.00],[34.5,0.00],[35.5,-0.20],[36.5,-0.20],[37.5,-0.40],[38.5,-0.20],[39.5,-0.20],[40.5,0.00],[41.5,0.00],[42.5,0.00],[43.5,-0.20],[44.5,-0.20],[45.5,-0.20],[46.5,-0.20],[47.5,0.20],[48.5,0.20],[49.5,0.20],[50.5,0.00],[51.5,0.00],[52.5,0.00],[53.5,0.00],[54.5,0.00],[55.5,0.40],[56.5,0.40],[57.5,0.20],[58.5,0.40],[59.5,0.40],[60.5,0.20],[61.5,0.20],[62.5,0.20],[63.5,0.00],[64.5,-0.20],[65.5,-0.20],[66.5,-0.40],[67.5,-0.40],[68.5,-0.20],[69.5,0.00],[70.5,0.00],[71.5,0.20],[72.5,0.20],[73.5,0.20],[74.5,0.20],[75.5,0.40],[76.5,0.20],[77.5,0.20],[78.5,0.00],[79.5,0.00],[80.5,-0.20],[81.5,0.00],[82.5,0.00],[83.5,0.00],[84.5,0.00],[85.5,0.00],[86.5,-0.20],[87.5,-0.20],[88.5,-0.40],[89.5,-0.40],[90.5,-0.40],[91.5,-0.20],[92.5,0.00],[93.5,0.20],[94.5,0.20],[95.5,0.20],[96.5,0.00],[97.5,-0.20],[98.5,-0.10],[99.5,-0.10],[100.5,-0.10],[101.5,0.10],[102.5,0.10],[103.5,-0.20],[104.5,-0.20],[105.5,-0.20],[106.5,0.00],[107.5,0.00],[108.5,0.20],[109.5,0.00],[110.5,0.00],[111.5,-0.20],[112.5,-0.20],[113.5,-0.20],[114.5,0.00],[115.5,0.00],[116.5,-0.20],[117.5,-0.20],[118.5,-0.20],[119.5,-0.20],[120.5,-0.20],[121.5,0.00],[122.5,0.00],[123.5,0.00],[124.5,-0.20],[125.5,-0.20],[126.5,-0.20],[127.5,-0.40],[128.5,-0.20],[129.5,0.00],[130.5,0.20],[131.5,0.20],[132.5,0.40],[133.5,0.20],[134.5,0.00],[135.5,-0.20],[136.5,-0.20],[137.5,-0.20],[138.5,-0.20],[139.5,0.00],[140.5,0.00],[141.5,0.00],[142.5,0.00],[143.5,0.20],[144.5,0.20],[145.5,0.40],[146.5,0.40],[147.5,0.40],[148.5,0.00],[149.5,0.00],[150.5,-0.20],[151.5,-0.20],[152.5,-0.20],[153.5,0.00],[154.5,0.20],[155.5,0.20],[156.5,0.20],[157.5,0.30],[158.5,0.10],[159.5,-0.10],[160.5,-0.10],[161.5,-0.10],[162.5,-0.20],[163.5,0.00],[164.5,0.20],[165.5,0.20],[166.5,0.20],[167.5,0.20],[168.5,0.20],[169.5,0.00],[170.5,-0.20],[171.5,-0.40],[172.5,-0.40],[173.5,-0.20],[174.5,0.00],[175.5,0.40],[176.5,0.60],[177.5,0.60],[178.5,0.40],[179.5,0.40],[180.5,0.20],[181.5,0.20],[182.5,0.40],[183.5,0.40],[184.5,0.20],[185.5,0.20],[186.5,0.00],[187.5,-0.20],[188.5,-0.20],[189.5,-0.20],[190.5,-0.20],[191.5,0.00],[192.5,0.00],[193.5,0.00],[194.5,0.00],[195.5,0.00],[196.5,0.00],[197.5,0.00],[198.5,-0.20],[199.5,-0.40],[200.5,-0.20],[201.5,-0.20],[202.5,-0.20],[203.5,0.00],[204.5,0.20],[205.5,0.10],[206.5,0.10],[207.5,0.10],[208.5,0.10],[209.5,0.10],[210.5,0.00],[211.5,-0.20],[212.5,-0.20],[213.5,-0.20],[214.5,-0.20],[215.5,-0.20],[216.5,-0.20],[217.5,-0.20],[218.5,-0.20],[219.5,0.00],[220.5,0.20],[221.5,0.40],[222.5,0.40],[223.5,0.60],[224.5,0.40],[225.5,0.20],[226.5,0.00],[227.5,0.00],[228.5,-0.20],[229.5,-0.40],[230.5,-0.40],[231.5,-0.20],[232.5,-0.20],[233.5,-0.20],[234.5,0.00],[235.5,-0.20],[236.5,-0.20],[237.5,-0.20],[238.5,-0.20],[239.5,-0.20],[240.5,-0.20],[241.5,-0.20],[242.5,-0.40],[243.5,-0.20],[244.5,-0.40],[245.5,-0.20],[246.5,-0.20],[247.5,0.00],[248.5,-0.20],[249.5,0.00],[250.5,-0.20],[251.5,-0.20],[252.5,-0.40],[253.5,-0.20],[254.5,-0.20],[255.5,0.00],[256.5,0.00],[257.5,0.20],[258.5,0.00],[259.5,0.00],[260.5,0.00],[261.5,0.00],[262.5,-0.20],[263.5,-0.20],[264.5,-0.20],[265.5,-0.20],[266.5,0.00],[267.5,0.20],[268.5,0.20],[269.5,0.30],[270.5,0.50],[271.5,0.40],[272.5,0.20],[273.5,0.20],[274.5,-0.10],[275.5,-0.30],[276.5,-0.40],[277.5,-0.20],[278.5,0.00],[279.5,0.20],[280.5,0.30],[281.5,0.30],[282.5,0.30],[283.5,0.10],[284.5,-0.10],[285.5,-0.40],[286.5,-0.40],[287.5,-0.40],[288.5,-0.60],[289.5,-0.40],[290.5,-0.20],[291.5,-0.20],[292.5,-0.00],[293.5,0.40],[294.5,0.40],[295.5,0.40],[296.5,0.40],[297.5,0.20],[298.5,-0.00],[299.5,-0.00],[300.5,-0.00],[301.5,-0.00],[302.5,-0.00],[303.5,-0.00],[304.5,-0.00],[305.5,-0.00],[306.5,-0.00],[307.5,-0.20],[308.5,-0.20],[309.5,-0.40],[310.5,-0.40],[311.5,-0.40],[312.5,-0.20],[313.5,-0.20],[314.5,-0.00],[315.5,-0.00],[316.5,-0.00],[317.5,-0.00],[318.5,-0.20],[319.5,-0.40],[320.5,-0.40],[321.5,-0.40],[322.5,-0.40],[323.5,-0.20],[324.5,-0.00],[325.5,-0.00],[326.5,-0.00],[327.5,-0.00],[328.5,-0.00],[329.5,0.20],[330.5,0.20],[331.5,0.20],[332.5,-0.00],[333.5,0.20],[334.5,0.20],[335.5,0.40],[336.5,0.40],[337.5,0.60],[338.5,0.40],[339.5,0.20],[340.5,0.20],[341.5,0.20],[342.5,0.20],[343.5,0.20],[344.5,0.20],[345.5,-0.00],[346.5,0.20],[347.5,0.20],[348.5,0.20],[349.5,0.20],[350.5,0.20],[351.5,0.20],[352.5,0.20],[353.5,0.20],[354.5,0.40],[355.5,0.60],[356.5,0.40],[357.5,0.40],[358.5,0.40],[359.5,0.20],[360.5,-0.00],[361.5,-0.00],[362.5,0.10],[363.5,0.10],[364.5,0.10],[365.5,0.10],[366.5,-0.10],[367.5,-0.00],[368.5,-0.00],[369.5,-0.20],[370.5,-0.20],[371.5,-0.00],[372.5,-0.20],[373.5,-0.20],[374.5,-0.00],[375.5,-0.00],[376.5,-0.00],[377.5,-0.00],[378.5,-0.00],[379.5,-0.00],[380.5,-0.00],[381.5,-0.00],[382.5,-0.00],[383.5,-0.00],[384.5,-0.00],[385.5,-0.00],[386.5,-0.00],[387.5,-0.00],[388.5,-0.00],[389.5,-0.00],[390.5,-0.00],[391.5,-0.00],[392.5,-0.00],[393.5,-0.00],[394.5,-0.00],[395.5,-0.00],[396.5,-0.00],[397.5,-0.00],[398.5,-0.00],[399.5,-0.00],[400.5,-0.00],[401.5,-0.00],[402.5,-0.00],[403.5,-0.00],[404.5,-0.20],[405.5,-0.20],[406.5,-0.20],[407.5,-0.20],[408.5,-0.20],[409.5,-0.00],[410.5,-0.00],[411.5,-0.00],[412.5,-0.00],[413.5,-0.00],[414.5,-0.00],[415.5,-0.00],[416.5,-0.00],[417.5,-0.00],[418.5,-0.00],[419.5,-0.00],[420.5,-0.00],[421.5,-0.00],[422.5,-0.00],[423.5,-0.00],[424.5,-0.00],[425.5,-0.00],[426.5,-0.00],[427.5,-0.00],[428.5,-0.00],[429.5,-0.00],[430.5,-0.00],[431.5,-0.00],[432.5,-0.00],[433.5,0.20],[434.5,0.40],[435.5,0.60],[436.5,0.80],[437.5,0.80],[438.5,0.60],[439.5,0.20],[440.5,0.00],[441.5,-0.20],[442.5,-0.20],[443.5,-0.20],[444.5,0.00],[445.5,0.00],[446.5,0.00],[447.5,0.00],[448.5,0.00],[449.5,0.00],[450.5,0.00],[451.5,0.00],[452.5,0.00],[453.5,0.00],[454.5,0.20],[455.5,0.20],[456.5,0.00],[457.5,-0.20],[458.5,-0.20],[459.5,-0.40],[460.5,-0.40],[461.5,-0.20],[462.5,0.00],[463.5,0.00],[464.5,0.00],[465.5,0.00],[466.5,0.00],[467.5,0.00],[468.5,-0.20],[469.5,-0.20],[470.5,-0.40],[471.5,-0.40],[472.5,-0.60],[473.5,-0.40],[474.5,-0.40],[475.5,-0.20],[476.5,-0.10],[477.5,0.30],[478.5,0.30],[479.5,0.30],[480.5,0.30],[481.5,0.20],[482.5,0.00],[483.5,-0.20],[484.5,-0.20],[485.5,-0.40],[486.5,-0.40],[487.5,-0.60],[488.5,-0.40],[489.5,-0.40],[490.5,-0.20],[491.5,-0.20],[492.5,0.20],[493.5,0.20],[494.5,0.20],[495.5,0.20],[496.5,0.40],[497.5,0.20],[498.5,0.20],[499.5,0.20],[500.5,0.20],[501.5,-0.00],[502.5,-0.00],[503.5,0.20],[504.5,0.20],[505.5,0.20],[506.5,0.20],[507.5,0.20],[508.5,-0.00],[509.5,-0.00],[510.5,-0.00],[511.5,-0.00],[512.5,-0.00],[513.5,-0.00],[514.5,-0.00],[515.5,-0.00],[516.5,-0.00],[517.5,-0.00],[518.5,-0.00],[519.5,-0.00],[520.5,-0.00],[521.5,-0.00],[522.5,-0.00],[523.5,-0.00],[524.5,0.20],[525.5,0.20],[526.5,0.20],[527.5,-0.00],[528.5,0.10],[529.5,-0.10],[530.5,0.10],[531.5,0.10],[532.5,0.40],[533.5,0.10],[534.5,0.10],[535.5,-0.10],[536.5,0.10],[537.5,-0.00],[538.5,0.20],[539.5,0.20],[540.5,0.20],[541.5,-0.20],[542.5,-0.20],[543.5,-0.20],[544.5,-0.20],[545.5,-0.20],[546.5,-0.20],[547.5,-0.00],[548.5,-0.00],[549.5,-0.00],[550.5,-0.00],[551.5,0.20],[552.5,0.20],[553.5,-0.00],[554.5,-0.20],[555.5,-0.20],[556.5,-0.20],[557.5,-0.40],[558.5,-0.00],[559.5,0.20],[560.5,0.40],[561.5,0.40],[562.5,0.20],[563.5,-0.00],[564.5,0.20],[565.5,-0.00],[566.5,-0.20],[567.5,-0.00],[568.5,-0.20],[569.5,-0.40],[570.5,-0.40],[571.5,-0.20],[572.5,-0.20],[573.5,0.20],[574.5,0.20],[575.5,-0.00],[576.5,-0.00],[577.5,0.20],[578.5,-0.00],[579.5,-0.00],[580.5,-0.00],[581.5,-0.00],[582.5,-0.00],[583.5,-0.20],[584.5,-0.40],[585.5,-0.20],[586.5,-0.20],[587.5,-0.40],[588.5,-0.20],[589.5,-0.00],[590.5,0.10],[591.5,0.30],[592.5,0.30],[593.5,0.10],[594.5,0.10],[595.5,-0.00],[596.5,-0.20],[597.5,-0.20],[598.5,-0.00],[599.5,0.10],[600.5,0.10],[601.5,0.10],[602.5,0.10],[603.5,0.10],[604.5,-0.00],[605.5,-0.00],[606.5,-0.20],[607.5,-0.00],[608.5,-0.00],[609.5,0.20],[610.5,0.20],[611.5,0.40],[612.5,0.20],[613.5,0.40],[614.5,0.20],[615.5,0.20],[616.5,0.20],[617.5,0.40],[618.5,0.20],[619.5,0.40],[620.5,0.50],[621.5,0.50],[622.5,0.30],[623.5,0.10],[624.5,-0.30],[625.5,-0.40],[626.5,-0.40],[627.5,-0.40],[628.5,-0.20],[629.5,-0.00],[630.5,-0.00],[631.5,-0.20],[632.5,-0.10],[633.5,-0.10],[634.5,-0.10],[635.5,-0.10],[636.5,0.10],[637.5,-0.00],[638.5,-0.00],[639.5,-0.00],[640.5,-0.00],[641.5,-0.20],[642.5,-0.20],[643.5,-0.20],[644.5,-0.20],[645.5,-0.20],[646.5,-0.00],[647.5,-0.00],[648.5,-0.20],[649.5,-0.20],[650.5,-0.10],[651.5,-0.10],[652.5,-0.10],[653.5,0.10],[654.5,0.10],[655.5,-0.00],[656.5,-0.00],[657.5,-0.00],[658.5,-0.00],[659.5,-0.00],[660.5,0.20],[661.5,-0.00],[662.5,-0.00],[663.5,-0.00],[664.5,-0.20],[665.5,-0.20],[666.5,0.20],[667.5,-0.00],[668.5,0.10],[669.5,0.30],[670.5,0.10],[671.5,-0.10],[672.5,0.10],[673.5,-0.20],[674.5,-0.20],[675.5,-0.20],[676.5,-0.20],[677.5,-0.20],[678.5,-0.00],[679.5,-0.00],[680.5,-0.00],[681.5,-0.00],[682.5,0.10],[683.5,0.10],[684.5,0.10],[685.5,-0.10],[686.5,-0.30],[687.5,-0.40],[688.5,-0.40],[689.5,-0.20],[690.5,-0.00],[691.5,0.20]];dis_datapoints = [];trans_datapoints = [];sec_helix_datapoints = [[44,56],[67,82],[103,113],[135,147],[163,168],[172,179],[181,192],[218,224],[264,273],[411,419],[423,438],[440,446],[459,463],[511,519],[538,540],[688,691]];sec_strand_datapoints = [[9,10],[87,90],[94,98],[121,125],[153,157],[196,200],[204,208],[236,241],[259,261],[278,282],[284,286],[290,293],[297,303],[315,321],[326,331],[467,476],[480,485],[495,502],[548,555],[559,568],[572,580],[603,607],[615,625],[630,637],[654,662],[672,679]];acc_exposed_datapoints = [[1,6],[8,10],[18,18],[20,20],[23,26],[28,29],[31,31],[36,36],[38,38],[40,41],[45,46],[49,49],[53,53],[57,58],[60,62],[64,64],[66,68],[72,72],[75,75],[79,79],[82,83],[85,85],[91,91],[112,112],[115,115],[118,119],[151,151],[177,177],[182,182],[185,185],[189,189],[192,192],[214,214],[219,219],[223,223],[227,227],[229,229],[232,233],[245,247],[249,250],[253,253],[255,255],[265,265],[269,269],[272,273],[275,275],[288,288],[295,295],[322,323],[335,340],[344,344],[350,350],[354,355],[357,358],[364,364],[367,367],[369,373],[375,375],[377,378],[380,386],[388,390],[392,392],[395,396],[398,399],[402,402],[405,407],[439,440],[442,442],[446,446],[449,449],[451,451],[453,454],[457,457],[460,460],[463,464],[467,467],[488,488],[490,490],[493,493],[495,495],[504,504],[527,527],[530,530],[533,533],[544,544],[569,569],[571,571],[583,583],[586,587],[594,594],[596,596],[598,599],[612,612],[618,618],[626,627],[629,629],[639,640],[644,645],[648,648],[651,651],[663,670],[686,686],[688,689],[692,694]];acc_buried_datapoints = [[44,44],[47,48],[51,52],[65,65],[70,70],[73,74],[77,77],[81,81],[86,88],[90,90],[92,92],[94,94],[96,105],[107,108],[110,111],[120,120],[122,132],[135,145],[147,147],[149,150],[152,162],[165,166],[168,171],[175,175],[179,179],[183,184],[187,188],[190,191],[193,200],[204,212],[215,215],[218,218],[221,221],[224,224],[235,242],[244,244],[257,263],[266,267],[270,271],[276,276],[278,283],[285,286],[289,290],[292,293],[298,308],[313,318],[320,320],[324,324],[326,328],[330,331],[352,352],[410,414],[417,418],[420,420],[422,424],[426,428],[430,435],[438,438],[444,444],[447,447],[455,455],[458,458],[461,462],[466,466],[468,468],[470,470],[472,472],[474,474],[476,479],[481,481],[483,483],[485,485],[487,487],[489,489],[491,491],[494,494],[496,496],[498,498],[500,500],[502,502],[507,522],[524,526],[528,529],[532,532],[537,538],[541,542],[545,547],[551,552],[554,554],[556,556],[558,560],[562,562],[564,564],[566,566],[568,568],[572,573],[575,575],[577,577],[579,579],[581,582],[584,584],[588,588],[590,591],[604,608],[613,613],[615,615],[617,617],[619,619],[621,623],[625,625],[628,628],[630,633],[635,635],[643,643],[647,647],[649,650],[652,657],[659,659],[661,661],[672,672],[674,674],[676,678],[680,680],[682,682],[687,687],[690,690]];flot_plot_options = []; flot_plot_options[0] = {grid: {borderWidth: {top: 0,right: 0,bottom: 0,left: 0}},legend: {show: false},xaxes: [{show: true,min: 0,max: 200,ticks: [[0.5, '1'], [24.5, '25'], [49.5, '50'], [74.5, '75'], [99.5, '100'], [124.5, '125'], [149.5, '150'], [174.5, '175'], [199.5, '200']],tickLength: -5}],yaxes: [{show: true,ticks: [[0, '0'], [4.5,'hydro-<br>phobic '], [-4.5,'hydro-<br>philic ']],min: -4.5,max: +4.5,font: {size: 12,lineHeight: 14,style: 'italic',weight: 'bold',family: 'sans-serif',variant: 'small-caps',color: 'rgba(100,149,237,1)'}},{show: true,ticks: [[0, ''], [1,'positive<br> charge'], [-1,'negative<br> charge']],position: 'right',min: -1,max: 1,font: {size: 12,lineHeight: 14,style: 'italic',weight: 'bold',family: 'sans-serif',variant: 'small-caps',color: 'rgba(255,99,71,1)'}}]};number_of_plots = 4;for ( plot_num = 1 ; plot_num < number_of_plots ; plot_num ++){flot_plot_options[plot_num] = $.extend(true, {} ,flot_plot_options[0]);flot_plot_options[plot_num].xaxes = [{min: plot_num*200,max: (plot_num + 1)*200,ticks: [ [plot_num*200 + 0.5, (plot_num*200 + 1).toString()], [plot_num*200 + 24.5, (plot_num*200 + 25).toString()], [plot_num*200 + 49.5, (plot_num*200 + 50).toString()], [plot_num*200 + 74.5, (plot_num*200 + 75).toString()], [plot_num*200 + 99.5, (plot_num*200 + 100).toString()], [plot_num*200 + 124.5, (plot_num*200 + 125).toString()], [plot_num*200 + 149.5, (plot_num*200 + 150).toString()], [plot_num*200 + 174.5, (plot_num*200 + 175).toString()], [plot_num*200 + 199.5, (plot_num*200 + 200).toString()] ],tickLength: -5}];};try {if( $('#AutoAnnotator_container_1381244609553 #hydrophobicity_charge_button').val() =='Show' ){$('#AutoAnnotator_container_1381244609553 #hydrophobicity_charge_container').css('display','block');$('#AutoAnnotator_container_1381244609553 #hydrophobicity_charge_button').val('Hide');var description_html = '<div id=\'AutoAnnotator_plot_selectors\'>';description_html = description_html + '<br> <input type=\'checkbox\' id=\'hydrophobicity_checkbox\' checked=\'checked\'> Moving average over 5 amino acids for hydrophobicity (<img src=\'https://static.igem.org/mediawiki/2013/e/e9/TUM13_hydrophobicity_icon.png\' alt=\'blue graph\' height=\'10\'></img>)';description_html = description_html + '<br> <input type=\'checkbox\' id=\'charge_checkbox\' checked=\'checked\'> Moving average over 5 amino acids for charge (<img src=\'https://static.igem.org/mediawiki/2013/3/3e/TUM13_charge_icon.png\' alt=\'red graph\' height=\'10\'></img>)';description_html = description_html + '<br> <input type=\'checkbox\' id=\'dis_checkbox\' checked=\'checked\'> Predicted disulfid bridges (<img src=\'https://static.igem.org/mediawiki/2013/2/28/TUM13_dis_icon.png\' alt=\'yellow circle\' height=\'10\'></img>) with the number of the bridge in the center';description_html = description_html + '<br> <input type=\'checkbox\' id=\'trans_checkbox\' checked=\'checked\'> Predicted transmembrane helices (<img src=\'https://static.igem.org/mediawiki/2013/7/78/TUM13_trans_icon.png\' alt=\'turquois bars\' height=\'10\'></img>)';description_html = description_html + '<br> <input type=\'checkbox\' id=\'sec_checkbox\' checked=\'checked\'> Predicted secondary structure: Helices (<img src=\'https://static.igem.org/mediawiki/2013/b/bf/TUM13_helix_icon.png\' alt=\'violet bars\' height=\'10\'></img>) and beta-strands (<img src=\'https://static.igem.org/mediawiki/2013/b/bf/TUM13_strand_icon.png\' alt=\'yellow bars\' height=\'10\'></img>)';description_html = description_html + '<br> <input type=\'checkbox\' id=\'acc_checkbox\' checked=\'checked\'> Predicted solvent accessability: Exposed (<img src=\'https://static.igem.org/mediawiki/2013/1/16/TUM13_exposed_icon.png\' alt=\'blue bars\' height=\'10\'></img>) and buried (<img src=\'https://static.igem.org/mediawiki/2013/0/0b/TUM13_buried_icon.png\' alt=\'green bars\' height=\'10\'></img>) residues';description_html = description_html + '<br></div>';$('#AutoAnnotator_container_1381244609553 #hydrophobicity_charge_explanation').html(description_html);plot_according_to_selectors_1381244609553();$('#AutoAnnotator_container_1381244609553 #AutoAnnotator_plot_selectors').find('input').click(plot_according_to_selectors_1381244609553);}else{$('#AutoAnnotator_container_1381244609553 #hydrophobicity_charge_container').css('display','none');$('#AutoAnnotator_container_1381244609553 #hydrophobicity_charge_button').val('Show');$('#AutoAnnotator_container_1381244609553 #hydrophobicity_charge_explanation').html('');}}catch(err){txt='There was an error with the button controlling the visibility of the plot.\n';txt=txt+'The originating error is:\n' + err + '\n\n';alert(txt);}};function plot_according_to_selectors_1381244609553(){try{var plot_datasets = [[],[]];if($('#AutoAnnotator_container_1381244609553 #hydrophobicity_checkbox').prop('checked') == true){plot_datasets[0] = { color: 'rgba(100,149,237,1)',data: hydrophobicity_datapoints,label: 'Hydrophobicity',lines: { show: true, fill: true, fillColor: 'rgba(100,149,237,0.1)' },yaxis: 1};}if($('#AutoAnnotator_container_1381244609553 #charge_checkbox').prop('checked') == true){plot_datasets[1] = {color: 'rgba(255,99,71,1)',data: charge_datapoints,label: 'Charge',lines: { show: true, fill: true, fillColor: 'rgba(255,99,71,0.1)' },yaxis: 2};}for (plot_num = 0 ; plot_num < number_of_plots ; plot_num ++){$.plot('#AutoAnnotator_container_1381244609553 #hydrophobicity_charge_placeholder'+ plot_num.toString(), plot_datasets, flot_plot_options[plot_num] );}var screen_width = $('canvas.flot-base').width(); var pos_of_first_tick = 46;var pos_of_last_tick = screen_width - 51;var tick_diff = (screen_width - 97)/199;if($('#AutoAnnotator_container_1381244609553 #dis_checkbox').prop('checked') == true){for ( j = 0 ; j < dis_datapoints.length ; j++ ){$('#AutoAnnotator_container_1381244609553 #hydrophobicity_charge_placeholder' + Math.floor((dis_datapoints[j][0] - 1)/200) ).append('<div class=\'AutoAnnotator_dis\' style=\'left:' + ((pos_of_first_tick - 8 + (dis_datapoints[j][0] - 1)*tick_diff - Math.floor((dis_datapoints[j][0] - 1)/200)*200*tick_diff).toFixed(0)).toString() + 'px;\'><b>' + (j+1) + '</b></div>');$('#AutoAnnotator_container_1381244609553 #hydrophobicity_charge_placeholder' + Math.floor((dis_datapoints[j][1] - 1)/200) ).append('<div class=\'AutoAnnotator_dis\' style=\'left:' + ((pos_of_first_tick - 8 + (dis_datapoints[j][1] - 1)*tick_diff - Math.floor((dis_datapoints[j][1] - 1)/200)*200*tick_diff).toFixed(0)).toString() + 'px;\'><b>' + (j+1) + '</b></div>');}}if($('#AutoAnnotator_container_1381244609553 #trans_checkbox').prop('checked') == true){for ( j = 0 ; j < trans_datapoints.length ; j++ ){$('#AutoAnnotator_container_1381244609553 #hydrophobicity_charge_placeholder' + Math.floor((trans_datapoints[j][0] - 1)/200) ).append('<div class=\'AutoAnnotator_trans\' style=\'width:' + (((trans_datapoints[j][1] - trans_datapoints[j][0] + 1)*tick_diff).toFixed(0)).toString() + 'px ;left:' + ((pos_of_first_tick + (trans_datapoints[j][0] - 1.5)*tick_diff - Math.floor((trans_datapoints[j][0] - 1)/200)*200*tick_diff).toFixed(0)).toString() + 'px\'></div>');}}if($('#AutoAnnotator_container_1381244609553 #sec_checkbox').prop('checked') == true){for ( j = 0 ; j < sec_helix_datapoints.length ; j++ ){$('#AutoAnnotator_container_1381244609553 #hydrophobicity_charge_placeholder' + Math.floor((sec_helix_datapoints[j][0] - 1)/200) ).append('<div class=\'AutoAnnotator_sec_helix\' style=\'width:' + (((sec_helix_datapoints[j][1] - sec_helix_datapoints[j][0] + 1)*tick_diff).toFixed(0)).toString() + 'px; left:' + ((pos_of_first_tick + (sec_helix_datapoints[j][0] - 1.5)*tick_diff - Math.floor((sec_helix_datapoints[j][0] - 1)/200)*200*tick_diff).toFixed(0)).toString() + 'px\'></div>');}for ( j = 0 ; j < sec_strand_datapoints.length ; j++ ){$('#AutoAnnotator_container_1381244609553 #hydrophobicity_charge_placeholder' + Math.floor((sec_strand_datapoints[j][0] - 1)/200) ).append('<div class=\'AutoAnnotator_sec_strand\' style=\'width:' + (((sec_strand_datapoints[j][1] - sec_strand_datapoints[j][0] + 1)*tick_diff).toFixed(0)).toString() + 'px; left:' + ((pos_of_first_tick + (sec_strand_datapoints[j][0] - 1.5)*tick_diff - Math.floor((sec_strand_datapoints[j][0] - 1)/200)*200*tick_diff).toFixed(0)).toString() + 'px\'></div>');}}if($('#AutoAnnotator_container_1381244609553 #acc_checkbox').prop('checked') == true){for ( j = 0 ; j < acc_buried_datapoints.length ; j++ ){$('#AutoAnnotator_container_1381244609553 #hydrophobicity_charge_placeholder' + Math.floor((acc_buried_datapoints[j][0] - 1)/200) ).append('<div class=\'AutoAnnotator_acc_buried\' style=\'width:' + (((acc_buried_datapoints[j][1] - acc_buried_datapoints[j][0] + 1)*tick_diff).toFixed(0)).toString() + 'px; left:' + ((pos_of_first_tick + (acc_buried_datapoints[j][0] - 1.5)*tick_diff - Math.floor((acc_buried_datapoints[j][0] - 1)/200)*200*tick_diff).toFixed(0)).toString() + 'px\'></div>');}for ( j = 0 ; j < acc_exposed_datapoints.length ; j++ ){$('#AutoAnnotator_container_1381244609553 #hydrophobicity_charge_placeholder' + Math.floor((acc_exposed_datapoints[j][0] - 1)/200) ).append('<div class=\'AutoAnnotator_acc_exposed\' style=\'width:' + (((acc_exposed_datapoints[j][1] - acc_exposed_datapoints[j][0] + 1)*tick_diff).toFixed(0)).toString() + 'px; left:' + ((pos_of_first_tick + (acc_exposed_datapoints[j][0] - 1.5)*tick_diff - Math.floor((acc_exposed_datapoints[j][0] - 1)/200)*200*tick_diff).toFixed(0)).toString() + 'px\'></div>');}}}catch(err){txt='There was an error while drawing the selected elements for the plot.\n';txt=txt+'The originating error is:\n' + err + '\n\n';throw(txt);}}</script></html> | |
<!-- Uncomment this to enable Functional Parameter display | <!-- Uncomment this to enable Functional Parameter display | ||
Latest revision as of 15:04, 8 October 2013
Membrane-anchored Human Protein Phosphatase 1 (PP1) in RFC[25] N-Part
This part codes for a membrane-anchored version of the Human Protein Phosphatase 1 (PP1) for the chassis Physcomitrella patens. Please see subparts for for further description. This construct is flanked by RFC[25] N-part prefix and suffix. Note: This means only protein fusions to the C-terminus of this part is possible, adding promoters (typically RFC[10]) into the prefix or terminators/IRES (typically RFC[10]) into the suffix of this part is nevertheless possible.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 643
Illegal BamHI site found at 107 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1092
Illegal BsaI.rc site found at 1030
Illegal BsaI.rc site found at 2010
| Protein data table for BioBrick BBa_ automatically created by the BioBrick-AutoAnnotator version 1.0 | ||||||||||||||||||||||||||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Nucleotide sequence in RFC 25 N-Part using the stop codon in the suffix, so ACCGGT was added (in italics) to the 3' end: (underlined part encodes the protein) AACAATGCCTGGT ... CTCTACAAAACCGGT ORF from nucleotide position 5 to 2086 (excluding stop-codon) | ||||||||||||||||||||||||||||||||||||||||||||||
Amino acid sequence: (RFC 25 scars in shown in bold, other sequence features underlined; both given below)
| ||||||||||||||||||||||||||||||||||||||||||||||
Sequence features: (with their position in the amino acid sequence, see the list of supported features)
| ||||||||||||||||||||||||||||||||||||||||||||||
Amino acid composition:
| ||||||||||||||||||||||||||||||||||||||||||||||
Amino acid counting
| Biochemical parameters
| |||||||||||||||||||||||||||||||||||||||||||||
| Plot for hydrophobicity, charge, predicted secondary structure, solvent accessability, transmembrane helices and disulfid bridges | ||||||||||||||||||||||||||||||||||||||||||||||
Codon usage
| ||||||||||||||||||||||||||||||||||||||||||||||
Alignments (obtained from PredictProtein.org)
| ||||||||||||||||||||||||||||||||||||||||||||||
| Predictions (obtained from PredictProtein.org) | ||||||||||||||||||||||||||||||||||||||||||||||
Subcellular Localization (reliability in brackets)
| Gene Ontology (reliability in brackets)
| |||||||||||||||||||||||||||||||||||||||||||||
Predicted features:
| ||||||||||||||||||||||||||||||||||||||||||||||
| The BioBrick-AutoAnnotator was created by TU-Munich 2013 iGEM team. For more information please see the documentation. If you have any questions, comments or suggestions, please leave us a comment. | ||||||||||||||||||||||||||||||||||||||||||||||