Difference between revisions of "Part:BBa K1159011"

Latest revision as of 14:49, 8 October 2013

Secretory Laccase (SERK-SigP_Laccase) in RFC[25] N-Part

Laccase from Bacillus pumilus, which degrades a wide variety of phenolic and non-phenolic compounds, is fused with a N-terminal signal peptide for secretion (signal peptide from SERK Receptor of Physcomitrella patens). This construct is flanked by RFC[25] N-part prefix and suffix. Note: This means only protein fusions to the C-terminus of this part is possible, adding promoters (typically RFC[10]) into the prefix or terminators/IRES (typically RFC[10]) into the suffix of this part is nevertheless possible.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 226
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

Protein data table for BioBrick BBa_ automatically created by the BioBrick-AutoAnnotator version 1.0

Nucleotide sequence in RFC 25 N-Part using the stop codon in the suffix, so ACCGGT was added (in italics) to the 3' end: (underlined part encodes the protein)
AACAATGCCTGGT ... GATATCATCACCGGT
ORF from nucleotide position 5 to 1636 (excluding stop-codon)

Amino acid sequence: (RFC 25 scars in shown in bold, other sequence features underlined; both given below)

1
101
201
301
401
501

MPGEVAWWRPLFLIALMPIGVLSNAEGDALNTTGNLEKFVDELPIPEVAKPVKKNPKQTYYEIAMEEVFLKVHRDLPPTKLWTYNGSLPGPTIHANRNEK
VKVKWMNKLPLKHFLPVDHTIHEGHHDEPEVKTVVHLHGGVTPASSDGYPEAWFSRDFEATGPFFEREVYEYPNHQQACTLWYHDHAMALTRLNVYAGLA
GFYLISDAFEKSLELPKGEYDIPLMIMDRTFQEDGALFYPSRPNNTPEDSDIPDPSIVPFFCGETILVNGKVWPYLEVEPRKYRFRILNASNTRTYELHL
DNDATILQIGSDGGFLPRPVHHQSFSIAPAERFDVIIDFSAYENKTITLKNKAGCGQEVNPETDANIMQFKVTRPLKGRAPKTLRPIFKPLPPLRPCRAD
KERTLTLTGTQDKYGRPILLLDNQFWNDPVTENPRLGSVEVWSIVNPTRGTHPIHLHLVQFRVIDRRPFDTEVYQSTGDIVYTGPNEAPPLHEQGYKDTI
QAHAGEVIRIIARFVPYSGRYVWHCHILEHEDYDMMRPMDIITG*

Sequence features: (with their position in the amino acid sequence, see the list of supported features)

RFC25 scar (shown in bold):

33 to 34, 161 to 162

Amino acid composition:

Ala (A)	30 (5.5%)
Arg (R)	30 (5.5%)
Asn (N)	25 (4.6%)
Asp (D)	32 (5.9%)

Cys (C)	5 (0.9%)
Gln (Q)	14 (2.6%)
Glu (E)	39 (7.2%)
Gly (G)	33 (6.1%)

His (H)	23 (4.2%)
Ile (I)	33 (6.1%)
Leu (L)	46 (8.5%)
Lys (K)	27 (5.0%)

Met (M)	11 (2.0%)
Phe (F)	25 (4.6%)
Pro (P)	50 (9.2%)
Ser (S)	19 (3.5%)

Thr (T)	35 (6.4%)
Trp (W)	10 (1.8%)
Tyr (Y)	22 (4.0%)
Val (V)	35 (6.4%)

Amino acid counting

	Total number:	544
	Positively charged (Arg+Lys):	57 (10.5%)
	Negatively charged (Asp+Glu):	71 (13.1%)
	Aromatic (Phe+His+Try+Tyr):	80 (14.7%)

Biochemical parameters

	Atomic composition:	C₂₈₂₈H₄₃₁₂N₇₅₆O₈₀₂S₁₆
	Molecular mass [Da]:	62246.9
	Theoretical pI:	5.92
	Extinction coefficient at 280 nm [M^-1 cm^-1]:	87780 / 88093 (all Cys red/ox)

Plot for hydrophobicity, charge, predicted secondary structure, solvent accessability, transmembrane helices and disulfid bridges

Codon usage

	Organism:	E. coli	B. subtilis	S. cerevisiae	A. thaliana	P. patens	Mammals
	Codon quality (CAI):	good (0.72)	good (0.75)	good (0.68)	good (0.75)	good (0.78)	good (0.67)

Alignments (obtained from PredictProtein.org)

	SwissProt:	P07788 (67% identity on 507 AAs), Q12737 (27% identity on 452 AAs)
	TrEML:	B4AIB1 (99% identity on 509 AAs), A8FAG9 (98% identity on 508 AAs)
	PDB:	1gsk (67% identity on 502 AAs), 1of0 (67% identity on 502 AAs)

Predictions (obtained from PredictProtein.org)

Subcellular Localization (reliability in brackets)

	Archaea:	cytosol (99%)
	Bacteria:	secreted (41%)
	Eukarya:	secreted (66%)

Gene Ontology (reliability in brackets)

	Molecular Function Ontology:	GO:0005507 (34%), GO:0005515 (23%)
	Biological Process Ontology:	GO:0055114 (37%), GO:0017000 (21%)

Predicted features:

	Disulfid bridges:	-
	Transmembrane helices:	-

The BioBrick-AutoAnnotator was created by TU-Munich 2013 iGEM team. For more information please see the documentation.
If you have any questions, comments or suggestions, please leave us a comment.

@@ Line 10: / Line 10: @@
 <span class='h3bb'>Sequence and Features</span>
 <partinfo>BBa_K1159011 SequenceAndFeatures</partinfo>
+<html><!--- Please copy this table containing parameters for BBa_ at the end of the parametrs section ahead of the references. ---><style type="text/css">table#AutoAnnotator {border:1px solid black; width:100%; border-collapse:collapse;} th#AutoAnnotatorHeader { border:1px solid black; width:100%; background-color: rgb(221, 221, 221);} td.AutoAnnotator1col { width:100%; border:1px solid black; } span.AutoAnnotatorSequence { font-family:'Courier New', Arial; } td.AutoAnnotatorSeqNum	{ text-align:right; width:2%; } td.AutoAnnotatorSeqSeq { width:98% } td.AutoAnnotatorSeqFeat1 { width:3% } td.AutoAnnotatorSeqFeat2a { width:27% } td.AutoAnnotatorSeqFeat2b { width:97% } td.AutoAnnotatorSeqFeat3 { width:70% } table.AutoAnnotatorNoBorder { border:0px; width:100%; border-collapse:collapse; } table.AutoAnnotatorWithBorder { border:1px solid black; width:100%; border-collapse:collapse; } td.AutoAnnotatorOuterAmino { border:0px solid black; width:20% } td.AutoAnnotatorInnerAmino { border:1px solid black; width:50% } td.AutoAnnotatorAminoCountingOuter { border:1px solid black; width:40%;  } td.AutoAnnotatorBiochemParOuter { border:1px solid black; width:60%; } td.AutoAnnotatorAminoCountingInner1 { width: 7.5% } td.AutoAnnotatorAminoCountingInner2 { width:62.5% } td.AutoAnnotatorAminoCountingInner3 { width:30% } td.AutoAnnotatorBiochemParInner1 { width: 5% } td.AutoAnnotatorBiochemParInner2 { width:55% } td.AutoAnnotatorBiochemParInner3 { width:40% } td.AutoAnnotatorCodonUsage1 { width: 3% } td.AutoAnnotatorCodonUsage2 { width:14.2% } td.AutoAnnotatorCodonUsage3 { width:13.8% } td.AutoAnnotatorAlignment1 { width: 3% } td.AutoAnnotatorAlignment2 { width: 10% } td.AutoAnnotatorAlignment3 { width: 87% } td.AutoAnnotatorLocalizationOuter {border:1px solid black; width:40%} td.AutoAnnotatorGOOuter {border:1px solid black; width:60%} td.AutoAnnotatorLocalization1 { width: 7.5% } td.AutoAnnotatorLocalization2 { width: 22.5% } td.AutoAnnotatorLocalization3 { width: 70% } td.AutoAnnotatorGO1 { width: 5% } td.AutoAnnotatorGO2 { width: 35% } td.AutoAnnotatorGO3 { width: 60% } td.AutoAnnotatorPredFeat1 { width:3% } td.AutoAnnotatorPredFeat2a { width:27% } td.AutoAnnotatorPredFeat3 { width:70% } div.AutoAnnotator_trans { position:absolute; background:rgb(11,140,143); background-color:rgba(11,140,143, 0.8); height:5px; top:100px; } div.AutoAnnotator_sec_helix { position:absolute; background:rgb(102,0,102); background-color:rgba(102,0,102, 0.8); height:5px; top:110px; } div.AutoAnnotator_sec_strand { position:absolute; background:rgb(245,170,26); background-color:rgba(245,170,26, 1); height:5px; top:110px; } div.AutoAnnotator_acc_buried { position:absolute; background:rgb(89,168,15); background-color:rgba(89,168,15, 0.8); height:5px; top:120px; } div.AutoAnnotator_acc_exposed { position:absolute; background:rgb(0, 0, 255); background-color:rgba(0, 0, 255, 0.8); height:5px; top:120px; } div.AutoAnnotator_dis { position:absolute; text-align:center; font-family:Arial,Helvetica,sans-serif; background:rgb(255, 200, 0); background-color:rgba(255, 200, 0, 1); height:16px; width:16px; top:80px; border-radius:50%; } </style><div id='AutoAnnotator_container_1381243726671'><table id="AutoAnnotator"><tr><!-- Time stamp in ms since 1/1/1970 1381243726671 --><th id="AutoAnnotatorHeader" colspan="2">Protein data table for BioBrick <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_<!------------------------Enter BioBrick number here------------------------>">BBa_<!------------------------Enter BioBrick number here------------------------></a> automatically created by the <a href="http://2013.igem.org/Team:TU-Munich/Results/AutoAnnotator">BioBrick-AutoAnnotator</a> version 1.0</th></tr><tr><td class="AutoAnnotator1col" colspan="2"><strong>Nucleotide sequence</strong> in <strong>RFC 25 N-Part</strong> using the stop codon in the suffix, so ACCGGT was added (in italics) to the 3' end: (underlined part encodes the protein)<br><span class="AutoAnnotatorSequence">&nbsp;AACA<u>ATGCCTGGT&nbsp;...&nbsp;GATATCATC<i>ACCGGT</i></u></span><br>&nbsp;<strong>ORF</strong> from nucleotide position 5 to 1636 (excluding stop-codon)</td></tr><tr><td class="AutoAnnotator1col" colspan="2"><strong>Amino acid sequence:</strong> (RFC 25 scars in shown in bold, other sequence features underlined; both given below)<br><span class="AutoAnnotatorSequence"><table class="AutoAnnotatorNoBorder"><tr><td class="AutoAnnotatorSeqNum">1&nbsp;<br>101&nbsp;<br>201&nbsp;<br>301&nbsp;<br>401&nbsp;<br>501&nbsp;</td><td class="AutoAnnotatorSeqSeq">MPGEVAWWRPLFLIALMPIGVLSNAEGDALNT<b>TG</b>NLEKFVDELPIPEVAKPVKKNPKQTYYEIAMEEVFLKVHRDLPPTKLWTYNGSLPGPTIHANRNEK<br>VKVKWMNKLPLKHFLPVDHTIHEGHHDEPEVKTVVHLHGGVTPASSDGYPEAWFSRDFEA<b>TG</b>PFFEREVYEYPNHQQACTLWYHDHAMALTRLNVYAGLA<br>GFYLISDAFEKSLELPKGEYDIPLMIMDRTFQEDGALFYPSRPNNTPEDSDIPDPSIVPFFCGETILVNGKVWPYLEVEPRKYRFRILNASNTRTYELHL<br>DNDATILQIGSDGGFLPRPVHHQSFSIAPAERFDVIIDFSAYENKTITLKNKAGCGQEVNPETDANIMQFKVTRPLKGRAPKTLRPIFKPLPPLRPCRAD<br>KERTLTLTGTQDKYGRPILLLDNQFWNDPVTENPRLGSVEVWSIVNPTRGTHPIHLHLVQFRVIDRRPFDTEVYQSTGDIVYTGPNEAPPLHEQGYKDTI<br>QAHAGEVIRIIARFVPYSGRYVWHCHILEHEDYDMMRPMDIITG*</td></tr></table></span></td></tr><tr><td class="AutoAnnotator1col" colspan="2"><strong>Sequence features:</strong> (with their position in the amino acid sequence, see the <a href="http://2013.igem.org/Team:TU-Munich/Results/Software/FeatureList">list of supported features</a>)<table class="AutoAnnotatorNoBorder"><tr><td class="AutoAnnotatorSeqFeat1"></td><td class="AutoAnnotatorSeqFeat2a">RFC25 scar (shown in bold):&nbsp;</td><td class="AutoAnnotatorSeqFeat3">33 to 34, 161 to 162</td></tr></table></td></tr><tr><td class="AutoAnnotator1col" colspan="2"><strong>Amino acid composition:</strong><table class="AutoAnnotatorNoBorder"><tr><td class="AutoAnnotatorOuterAmino"><table class="AutoAnnotatorWithBorder"><tr><td class="AutoAnnotatorInnerAmino">Ala (A)</td><td class="AutoAnnotatorInnerAmino">30 (5.5%)</td></tr><tr><td class="AutoAnnotatorInnerAmino">Arg (R)</td><td class="AutoAnnotatorInnerAmino">30 (5.5%)</td></tr><tr><td class="AutoAnnotatorInnerAmino">Asn (N)</td><td class="AutoAnnotatorInnerAmino">25 (4.6%)</td></tr><tr><td class="AutoAnnotatorInnerAmino">Asp (D)</td><td class="AutoAnnotatorInnerAmino">32 (5.9%)</td></tr></table></td><td class="AutoAnnotatorOuterAmino"><table class="AutoAnnotatorWithBorder"><tr><td class="AutoAnnotatorInnerAmino">Cys (C)</td><td class="AutoAnnotatorInnerAmino">5 (0.9%)</td></tr><tr><td class="AutoAnnotatorInnerAmino">Gln (Q)</td><td class="AutoAnnotatorInnerAmino">14 (2.6%)</td></tr><tr><td class="AutoAnnotatorInnerAmino">Glu (E)</td><td class="AutoAnnotatorInnerAmino">39 (7.2%)</td></tr><tr><td class="AutoAnnotatorInnerAmino">Gly (G)</td><td class="AutoAnnotatorInnerAmino">33 (6.1%)</td></tr></table></td><td class="AutoAnnotatorOuterAmino"><table class="AutoAnnotatorWithBorder"><tr><td class="AutoAnnotatorInnerAmino">His (H)</td><td class="AutoAnnotatorInnerAmino">23 (4.2%)</td></tr><tr><td class="AutoAnnotatorInnerAmino">Ile (I)</td><td class="AutoAnnotatorInnerAmino">33 (6.1%)</td></tr><tr><td class="AutoAnnotatorInnerAmino">Leu (L)</td><td class="AutoAnnotatorInnerAmino">46 (8.5%)</td></tr><tr><td class="AutoAnnotatorInnerAmino">Lys (K)</td><td class="AutoAnnotatorInnerAmino">27 (5.0%)</td></tr></table></td><td class="AutoAnnotatorOuterAmino"><table class="AutoAnnotatorWithBorder"><tr><td class="AutoAnnotatorInnerAmino">Met (M)</td><td class="AutoAnnotatorInnerAmino">11 (2.0%)</td></tr><tr><td class="AutoAnnotatorInnerAmino">Phe (F)</td><td class="AutoAnnotatorInnerAmino">25 (4.6%)</td></tr><tr><td class="AutoAnnotatorInnerAmino">Pro (P)</td><td class="AutoAnnotatorInnerAmino">50 (9.2%)</td></tr><tr><td class="AutoAnnotatorInnerAmino">Ser (S)</td><td class="AutoAnnotatorInnerAmino">19 (3.5%)</td></tr></table></td><td class="AutoAnnotatorOuterAmino"><table class="AutoAnnotatorWithBorder"><tr><td class="AutoAnnotatorInnerAmino">Thr (T)</td><td class="AutoAnnotatorInnerAmino">35 (6.4%)</td></tr><tr><td class="AutoAnnotatorInnerAmino">Trp (W)</td><td class="AutoAnnotatorInnerAmino">10 (1.8%)</td></tr><tr><td class="AutoAnnotatorInnerAmino">Tyr (Y)</td><td class="AutoAnnotatorInnerAmino">22 (4.0%)</td></tr><tr><td class="AutoAnnotatorInnerAmino">Val (V)</td><td class="AutoAnnotatorInnerAmino">35 (6.4%)</td></tr></table></td></tr></table></td></tr><tr><td class="AutoAnnotatorAminoCountingOuter"><strong>Amino acid counting</strong><table class="AutoAnnotatorNoBorder"><tr><td class="AutoAnnotatorAminoCountingInner1"></td><td class="AutoAnnotatorAminoCountingInner2">Total number:</td><td class="AutoAnnotatorAminoCountingInner3">544</td></tr><tr><td class="AutoAnnotatorAminoCountingInner1"></td><td class="AutoAnnotatorAminoCountingInner2">Positively charged (Arg+Lys):</td><td class="AutoAnnotatorAminoCountingInner3">57 (10.5%)</td></tr><tr><td class="AutoAnnotatorAminoCountingInner1"></td><td class="AutoAnnotatorAminoCountingInner2">Negatively charged (Asp+Glu):</td><td class="AutoAnnotatorAminoCountingInner3">71 (13.1%)</td></tr><tr><td class="AutoAnnotatorAminoCountingInner1"></td><td class="AutoAnnotatorAminoCountingInner2">Aromatic (Phe+His+Try+Tyr):</td><td class="AutoAnnotatorAminoCountingInner3">80 (14.7%)</td></tr></table></td><td class="AutoAnnotatorBiochemParOuter"><strong>Biochemical parameters</strong><table class="AutoAnnotatorNoBorder"><tr><td class="AutoAnnotatorBiochemParInner1"></td><td class="AutoAnnotatorBiochemParInner2">Atomic composition:</td><td class="AutoAnnotatorBiochemParInner3">C<sub>2828</sub>H<sub>4312</sub>N<sub>756</sub>O<sub>802</sub>S<sub>16</sub></td></tr><tr><td class="AutoAnnotatorBiochemParInner1"></td><td class="AutoAnnotatorBiochemParInner2">Molecular mass [Da]:</td><td class="AutoAnnotatorBiochemParInner3">62246.9</td></tr><tr><td class="AutoAnnotatorBiochemParInner1"></td><td class="AutoAnnotatorBiochemParInner2">Theoretical pI:</td><td class="AutoAnnotatorBiochemParInner3">5.92</td></tr><tr><td class="AutoAnnotatorBiochemParInner1"></td><td class="AutoAnnotatorBiochemParInner2">Extinction coefficient at 280 nm [M<sup>-1</sup> cm<sup>-1</sup>]:</td><td class="AutoAnnotatorBiochemParInner3">87780 / 88093 (all Cys red/ox)</td></tr></table></td></tr><tr><td class="AutoAnnotator1col" colspan="2"><strong>Plot for hydrophobicity, charge, predicted secondary structure, solvent accessability, transmembrane helices and disulfid bridges</strong>&nbsp;<input type='button' id='hydrophobicity_charge_button' onclick='show_or_hide_plot_1381243726671()' value='Show'><span id="hydrophobicity_charge_explanation"></span><div id="hydrophobicity_charge_container" style='display:none'><div id="hydrophobicity_charge_placeholder0" style="width:100%;height:150px"></div><div id="hydrophobicity_charge_placeholder1" style="width:100%;height:150px"></div><div id="hydrophobicity_charge_placeholder2" style="width:100%;height:150px"></div></div></td></tr><tr><td class="AutoAnnotator1col" colspan="2"><strong>Codon usage</strong><table class="AutoAnnotatorNoBorder"><tr><td class="AutoAnnotatorCodonUsage1"></td><td class="AutoAnnotatorCodonUsage2">Organism:</td><td class="AutoAnnotatorCodonUsage3"><i>E. coli</i></td><td class="AutoAnnotatorCodonUsage3"><i>B. subtilis</i></td><td class="AutoAnnotatorCodonUsage3"><i>S. cerevisiae</i></td><td class="AutoAnnotatorCodonUsage3"><i>A. thaliana</i></td><td class="AutoAnnotatorCodonUsage3"><i>P. patens</i></td><td class="AutoAnnotatorCodonUsage3">Mammals</td></tr><tr><td class="AutoAnnotatorCodonUsage1"></td><td class="AutoAnnotatorCodonUsage2">Codon quality (<a href="http://en.wikipedia.org/wiki/Codon_Adaptation_Index">CAI</a>):</td><td class="AutoAnnotatorCodonUsage3">good (0.72)</td><td class="AutoAnnotatorCodonUsage3">good (0.75)</td><td class="AutoAnnotatorCodonUsage3">good (0.68)</td><td class="AutoAnnotatorCodonUsage3">good (0.75)</td><td class="AutoAnnotatorCodonUsage3">good (0.78)</td><td class="AutoAnnotatorCodonUsage3">good (0.67)</td></tr></table></td></tr><tr><td class="AutoAnnotator1col" colspan="2"><strong>Alignments</strong> (obtained from <a href='http://predictprotein.org'>PredictProtein.org</a>)<table class="AutoAnnotatorNoBorder"><tr><td class="AutoAnnotatorAlignment1"></td><td class="AutoAnnotatorAlignment2">SwissProt:</td><td class="AutoAnnotatorAlignment3"><a href='http://www.uniprot.org/uniprot/P07788'>P07788</a> (67% identity on 507 AAs), <a href='http://www.uniprot.org/uniprot/Q12737'>Q12737</a> (27% identity on 452 AAs)</td></tr><tr><td class="AutoAnnotatorAlignment1"></td><td class="AutoAnnotatorAlignment2">TrEML:</td><td class="AutoAnnotatorAlignment3"><a href='http://www.uniprot.org/uniprot/B4AIB1'>B4AIB1</a> (99% identity on 509 AAs), <a href='http://www.uniprot.org/uniprot/A8FAG9'>A8FAG9</a> (98% identity on 508 AAs)</td></tr><tr><td class="AutoAnnotatorAlignment1"></td><td class="AutoAnnotatorAlignment2">PDB:</td><td class="AutoAnnotatorAlignment3"><a href='http://www.rcsb.org/pdb/explore/explore.do?structureId=1gsk'>1gsk</a> (67% identity on 502 AAs), <a href='http://www.rcsb.org/pdb/explore/explore.do?structureId=1of0'>1of0</a> (67% identity on 502 AAs)</td></tr></table></td></tr><tr><th id='AutoAnnotatorHeader' colspan="2"><strong>Predictions</strong> (obtained from <a href='http://predictprotein.org'>PredictProtein.org</a>)</th></tr><tr><td class="AutoAnnotatorLocalizationOuter"><strong>Subcellular Localization</strong> (reliability in brackets)<table class="AutoAnnotatorNoBorder"><tr><td class="AutoAnnotatorLocalization1"></td><td class="AutoAnnotatorLocalization2">Archaea:</td><td class="AutoAnnotatorLocalization3">cytosol (99%)</td></tr><tr><td class="AutoAnnotatorLocalization1"></td><td class="AutoAnnotatorLocalization2">Bacteria:</td><td class="AutoAnnotatorLocalization3">secreted (41%)</td></tr><tr><td class="AutoAnnotatorLocalization1"></td><td class="AutoAnnotatorLocalization2">Eukarya:</td><td class="AutoAnnotatorLocalization3">secreted (66%)</td></tr></table></td><td class="AutoAnnotatorGOOuter"><strong>Gene Ontology</strong> (reliability in brackets)<br><table class="AutoAnnotatorNoBorder"><tr><td class='AutoAnnotatorGO1'></td><td class='AutoAnnotatorGO2'>Molecular Function Ontology:</td><td class='AutoAnnotatorGO3'><a href='http://amigo.geneontology.org/cgi-bin/amigo/term_details?term=GO:0005507'>GO:0005507</a> (34%), <a href='http://amigo.geneontology.org/cgi-bin/amigo/term_details?term=GO:0005515'>GO:0005515</a> (23%)</td></tr><tr><td class='AutoAnnotatorGO1'></td><td class='AutoAnnotatorGO2'>Biological Process Ontology:</td><td class='AutoAnnotatorGO3'><a href='http://amigo.geneontology.org/cgi-bin/amigo/term_details?term=GO:0055114'>GO:0055114</a> (37%), <a href='http://amigo.geneontology.org/cgi-bin/amigo/term_details?term=GO:0017000'>GO:0017000</a> (21%)</td></tr><tr><td class='AutoAnnotatorGO1'> </td><td class='AutoAnnotatorGO2'> </td><td class='AutoAnnotatorGO3'>&nbsp;</td></tr></table></td></tr><tr><td class="AutoAnnotator1col" colspan="2"><strong>Predicted features:</strong><table class="AutoAnnotatorNoBorder"><tr><td class="AutoAnnotatorPredFeat1"></td><td class="AutoAnnotatorPredFeat2a">Disulfid bridges:</td><td class="AutoAnnotatorPredFeat3">&nbsp;- </td></tr><tr><td class="AutoAnnotatorPredFeat1"></td><td class="AutoAnnotatorPredFeat2a">Transmembrane helices:</td><td class="AutoAnnotatorPredFeat3">&nbsp;- </td></tr></table></td></tr><tr><td class="AutoAnnotator1col" colspan="2"> The BioBrick-AutoAnnotator was created by <a href="http://2013.igem.org/Team:TU-Munich">TU-Munich 2013</a> iGEM team. For more information please see the <a href="http://2013.igem.org/Team:TU-Munich/Results/Software">documentation</a>.<br>If you have any questions, comments or suggestions, please leave us a <a href="http://2013.igem.org/Team:TU-Munich/Results/AutoAnnotator">comment</a>.</td></tr></table></div><br><!-- IMPORTANT: DON'T REMOVE THIS LINE, OTHERWISE NOT SUPPORTED FOR IE BEFORE 9 --><!--[if lte IE 8]><script language="javascript" type="text/javascript" src="excanvas.min.js"></script><![endif]--><script type='text/javascript' src='http://code.jquery.com/jquery-1.10.0.min.js'></script><script type='text/javascript' src='http://2013.igem.org/Team:TU-Munich/Flot.js?action=raw&ctype=text/js'></script><script>function show_or_hide_plot_1381243726671(){hydrophobicity_datapoints = [[2.5,0.12],[3.5,0.10],[4.5,0.24],[5.5,0.14],[6.5,-0.06],[7.5,-1.22],[8.5,-0.82],[9.5,-0.08],[10.5,0.86],[11.5,2.66],[12.5,3.34],[13.5,3.34],[14.5,3.16],[15.5,2.08],[16.5,2.08],[17.5,1.64],[18.5,1.72],[19.5,2.10],[20.5,2.26],[21.5,0.66],[22.5,1.10],[23.5,-0.44],[24.5,-1.28],[25.5,-1.82],[26.5,-0.76],[27.5,-0.36],[28.5,-0.36],[29.5,-0.42],[30.5,0.14],[31.5,-0.30],[32.5,-1.76],[33.5,-0.30],[34.5,-0.86],[35.5,-1.50],[36.5,-0.86],[37.5,0.68],[38.5,-0.78],[39.5,-0.78],[40.5,0.76],[41.5,-0.12],[42.5,-0.06],[43.5,0.32],[44.5,0.32],[45.5,0.40],[46.5,1.08],[47.5,-0.60],[48.5,-0.60],[49.5,0.94],[50.5,-0.68],[51.5,-1.82],[52.5,-1.74],[53.5,-1.74],[54.5,-3.36],[55.5,-3.28],[56.5,-2.64],[57.5,-2.20],[58.5,-2.14],[59.5,-2.06],[60.5,-0.46],[61.5,0.04],[62.5,0.68],[63.5,0.24],[64.5,0.24],[65.5,0.18],[66.5,0.38],[67.5,0.76],[68.5,0.68],[69.5,2.22],[70.5,0.74],[71.5,-0.72],[72.5,-2.18],[73.5,-0.64],[74.5,-1.80],[75.5,-1.48],[76.5,-0.72],[77.5,-0.80],[78.5,-0.80],[79.5,-0.66],[80.5,-0.48],[81.5,-0.60],[82.5,-0.52],[83.5,-1.36],[84.5,-1.34],[85.5,-0.44],[86.5,-0.50],[87.5,0.12],[88.5,-0.12],[89.5,-0.10],[90.5,0.04],[91.5,-0.28],[92.5,0.16],[93.5,-0.22],[94.5,-0.98],[95.5,-2.58],[96.5,-2.64],[97.5,-3.78],[98.5,-2.24],[99.5,-2.12],[100.5,-0.58],[101.5,-0.66],[102.5,-0.06],[103.5,-0.52],[104.5,-0.44],[105.5,-2.06],[106.5,-0.52],[107.5,-0.66],[108.5,-0.28],[109.5,-0.36],[110.5,-0.22],[111.5,-0.42],[112.5,0.66],[113.5,-0.42],[114.5,1.20],[115.5,1.14],[116.5,-0.06],[117.5,-0.96],[118.5,0.26],[119.5,-1.22],[120.5,-1.22],[121.5,-0.66],[122.5,-1.16],[123.5,-2.70],[124.5,-2.76],[125.5,-2.76],[126.5,-3.00],[127.5,-3.06],[128.5,-1.58],[129.5,-1.66],[130.5,-1.10],[131.5,0.06],[132.5,1.60],[133.5,0.12],[134.5,1.66],[135.5,1.16],[136.5,0.24],[137.5,-0.68],[138.5,0.80],[139.5,-0.10],[140.5,0.22],[141.5,0.66],[142.5,0.58],[143.5,-0.42],[144.5,-0.98],[145.5,-0.74],[146.5,-1.36],[147.5,-1.52],[148.5,-2.06],[149.5,-1.00],[150.5,-1.10],[151.5,-0.28],[152.5,-0.12],[153.5,-0.32],[154.5,-1.38],[155.5,-0.64],[156.5,-1.90],[157.5,-1.38],[158.5,-0.62],[159.5,-0.00],[160.5,-0.88],[161.5,0.38],[162.5,0.58],[163.5,0.02],[164.5,-0.80],[165.5,-1.18],[166.5,-0.90],[167.5,-1.72],[168.5,-1.72],[169.5,-1.08],[170.5,-0.70],[171.5,-2.24],[172.5,-2.62],[173.5,-2.62],[174.5,-3.06],[175.5,-2.38],[176.5,-1.18],[177.5,-0.68],[178.5,0.78],[179.5,1.30],[180.5,0.68],[181.5,-0.46],[182.5,-1.02],[183.5,-2.42],[184.5,-1.88],[185.5,-1.24],[186.5,-0.24],[187.5,1.22],[188.5,1.72],[189.5,0.46],[190.5,0.84],[191.5,-0.22],[192.5,-0.14],[193.5,-0.26],[194.5,1.00],[195.5,0.16],[196.5,1.62],[197.5,1.14],[198.5,1.32],[199.5,1.52],[200.5,1.34],[201.5,1.34],[202.5,1.88],[203.5,1.80],[204.5,0.54],[205.5,1.16],[206.5,0.96],[207.5,-0.64],[208.5,-1.26],[209.5,-0.72],[210.5,-0.32],[211.5,-1.58],[212.5,-0.12],[213.5,0.34],[214.5,-0.28],[215.5,-1.12],[216.5,-1.12],[217.5,-2.14],[218.5,-2.52],[219.5,-0.84],[220.5,-1.08],[221.5,0.38],[222.5,1.02],[223.5,2.62],[224.5,2.10],[225.5,1.72],[226.5,0.06],[227.5,-0.46],[228.5,-0.80],[229.5,-1.88],[230.5,-1.88],[231.5,-1.68],[232.5,-1.62],[233.5,-1.82],[234.5,-0.36],[235.5,0.90],[236.5,1.34],[237.5,1.10],[238.5,0.58],[239.5,-1.08],[240.5,-1.96],[241.5,-2.40],[242.5,-2.78],[243.5,-2.76],[244.5,-2.18],[245.5,-2.56],[246.5,-2.56],[247.5,-2.02],[248.5,-2.58],[249.5,-1.36],[250.5,-0.98],[251.5,-0.98],[252.5,-1.14],[253.5,-0.60],[254.5,-0.60],[255.5,0.56],[256.5,0.94],[257.5,1.82],[258.5,2.54],[259.5,2.14],[260.5,1.22],[261.5,0.84],[262.5,0.14],[263.5,0.48],[264.5,0.74],[265.5,1.66],[266.5,1.66],[267.5,1.72],[268.5,0.04],[269.5,0.12],[270.5,-0.90],[271.5,-0.52],[272.5,-0.70],[273.5,0.84],[274.5,-0.70],[275.5,0.32],[276.5,-0.06],[277.5,-0.12],[278.5,-1.78],[279.5,-1.86],[280.5,-2.96],[281.5,-3.16],[282.5,-2.28],[283.5,-2.28],[284.5,-0.60],[285.5,0.42],[286.5,0.62],[287.5,0.42],[288.5,1.16],[289.5,-0.44],[290.5,-1.34],[291.5,-1.54],[292.5,-2.04],[293.5,-2.14],[294.5,-2.14],[295.5,-1.24],[296.5,-0.98],[297.5,-0.08],[298.5,-0.52],[299.5,-0.52],[300.5,-1.98],[301.5,-0.98],[302.5,-1.88],[303.5,-0.28],[304.5,1.18],[305.5,1.18],[306.5,1.72],[307.5,1.78],[308.5,0.72],[309.5,-0.74],[310.5,-0.12],[311.5,-1.10],[312.5,-0.46],[313.5,0.46],[314.5,0.84],[315.5,0.02],[316.5,-0.22],[317.5,0.06],[318.5,-1.34],[319.5,-1.66],[320.5,-1.46],[321.5,-1.30],[322.5,-1.58],[323.5,-1.10],[324.5,0.44],[325.5,1.50],[326.5,1.34],[327.5,1.14],[328.5,0.60],[329.5,-1.20],[330.5,-1.00],[331.5,-1.38],[332.5,-0.90],[333.5,0.70],[334.5,2.50],[335.5,1.24],[336.5,2.50],[337.5,1.50],[338.5,0.96],[339.5,-0.20],[340.5,-0.20],[341.5,-1.46],[342.5,-2.08],[343.5,-2.58],[344.5,-1.42],[345.5,-0.86],[346.5,0.60],[347.5,0.60],[348.5,0.04],[349.5,-1.64],[350.5,-1.14],[351.5,-1.98],[352.5,-0.70],[353.5,-0.08],[354.5,-0.00],[355.5,-1.06],[356.5,-0.14],[357.5,-1.34],[358.5,-1.58],[359.5,-1.58],[360.5,-1.02],[361.5,-2.56],[362.5,-1.50],[363.5,-1.88],[364.5,-0.28],[365.5,0.24],[366.5,0.24],[367.5,0.44],[368.5,0.36],[369.5,0.30],[370.5,-0.22],[371.5,-0.42],[372.5,-1.30],[373.5,0.24],[374.5,-1.38],[375.5,-1.32],[376.5,-1.32],[377.5,-0.64],[378.5,-1.72],[379.5,-1.72],[380.5,-1.78],[381.5,-0.12],[382.5,-1.38],[383.5,-1.38],[384.5,0.30],[385.5,1.00],[386.5,-0.54],[387.5,0.04],[388.5,1.12],[389.5,-0.10],[390.5,-0.98],[391.5,0.56],[392.5,-0.02],[393.5,-1.10],[394.5,-0.28],[395.5,-0.86],[396.5,-1.26],[397.5,-1.06],[398.5,-1.52],[399.5,-2.72],[400.5,-2.72],[401.5,-3.22],[402.5,-1.76],[403.5,-1.12],[404.5,0.34],[405.5,1.10],[406.5,1.16],[407.5,0.26],[408.5,-0.30],[409.5,-1.76],[410.5,-2.40],[411.5,-2.58],[412.5,-2.52],[413.5,-2.72],[414.5,-2.34],[415.5,-0.66],[416.5,0.36],[417.5,1.20],[418.5,2.86],[419.5,2.48],[420.5,0.88],[421.5,-0.58],[422.5,-0.78],[423.5,-1.72],[424.5,-1.72],[425.5,-1.72],[426.5,-1.34],[427.5,-1.06],[428.5,-1.02],[429.5,-1.02],[430.5,-1.02],[431.5,-1.02],[432.5,-2.76],[433.5,-1.86],[434.5,-1.24],[435.5,-0.70],[436.5,0.46],[437.5,0.66],[438.5,0.74],[439.5,0.64],[440.5,0.64],[441.5,0.70],[442.5,2.24],[443.5,0.70],[444.5,0.56],[445.5,0.58],[446.5,-1.22],[447.5,-2.14],[448.5,-1.58],[449.5,-1.90],[450.5,-2.08],[451.5,-0.28],[452.5,-0.84],[453.5,0.06],[454.5,0.06],[455.5,1.14],[456.5,1.08],[457.5,1.02],[458.5,0.82],[459.5,0.56],[460.5,0.64],[461.5,0.70],[462.5,0.70],[463.5,-0.76],[464.5,-0.76],[465.5,-1.92],[466.5,-2.26],[467.5,-2.26],[468.5,-1.50],[469.5,-1.30],[470.5,-0.14],[471.5,-0.96],[472.5,-0.96],[473.5,-0.98],[474.5,-0.42],[475.5,-1.34],[476.5,-1.78],[477.5,-0.18],[478.5,0.82],[479.5,0.70],[480.5,0.64],[481.5,1.26],[482.5,0.04],[483.5,-1.50],[484.5,-1.94],[485.5,-1.44],[486.5,-1.68],[487.5,-1.68],[488.5,-0.22],[489.5,-0.16],[490.5,-1.22],[491.5,-1.60],[492.5,-1.36],[493.5,-2.38],[494.5,-2.52],[495.5,-2.52],[496.5,-1.96],[497.5,-0.98],[498.5,-1.42],[499.5,-0.28],[500.5,-0.22],[501.5,0.28],[502.5,-0.70],[503.5,-0.70],[504.5,-0.22],[505.5,1.32],[506.5,0.06],[507.5,1.04],[508.5,2.64],[509.5,2.16],[510.5,0.36],[511.5,1.82],[512.5,1.76],[513.5,0.54],[514.5,-0.08],[515.5,0.66],[516.5,0.02],[517.5,-1.72],[518.5,-1.66],[519.5,-0.56],[520.5,-0.58],[521.5,-1.14],[522.5,0.26],[523.5,-0.12],[524.5,-0.06],[525.5,0.88],[526.5,0.82],[527.5,-0.32],[528.5,-0.38],[529.5,-1.98],[530.5,-3.00],[531.5,-3.00],[532.5,-1.98],[533.5,-0.90],[534.5,-1.10],[535.5,-1.16],[536.5,-0.08],[537.5,-1.16],[538.5,-0.64],[539.5,1.16],[540.5,1.34],[541.5,0.88]];charge_datapoints = [[2.5,-0.20],[3.5,-0.20],[4.5,-0.20],[5.5,-0.20],[6.5,0.20],[7.5,0.20],[8.5,0.20],[9.5,0.20],[10.5,0.20],[11.5,0.00],[12.5,0.00],[13.5,0.00],[14.5,0.00],[15.5,0.00],[16.5,0.00],[17.5,0.00],[18.5,0.00],[19.5,0.00],[20.5,0.00],[21.5,0.00],[22.5,0.00],[23.5,-0.20],[24.5,-0.20],[25.5,-0.40],[26.5,-0.40],[27.5,-0.40],[28.5,-0.20],[29.5,-0.20],[30.5,0.00],[31.5,0.00],[32.5,0.00],[33.5,0.00],[34.5,-0.20],[35.5,0.00],[36.5,0.00],[37.5,0.00],[38.5,-0.20],[39.5,-0.20],[40.5,-0.40],[41.5,-0.40],[42.5,-0.40],[43.5,-0.20],[44.5,-0.20],[45.5,-0.20],[46.5,-0.20],[47.5,0.00],[48.5,0.00],[49.5,0.20],[50.5,0.40],[51.5,0.60],[52.5,0.40],[53.5,0.40],[54.5,0.60],[55.5,0.40],[56.5,0.20],[57.5,0.20],[58.5,0.20],[59.5,-0.20],[60.5,-0.20],[61.5,-0.20],[62.5,-0.20],[63.5,-0.40],[64.5,-0.40],[65.5,-0.40],[66.5,-0.40],[67.5,-0.40],[68.5,0.00],[69.5,0.20],[70.5,0.30],[71.5,0.50],[72.5,0.30],[73.5,0.10],[74.5,0.10],[75.5,-0.00],[76.5,-0.20],[77.5,0.20],[78.5,0.20],[79.5,0.20],[80.5,0.20],[81.5,0.20],[82.5,-0.00],[83.5,-0.00],[84.5,-0.00],[85.5,-0.00],[86.5,-0.00],[87.5,-0.00],[88.5,-0.00],[89.5,-0.00],[90.5,-0.00],[91.5,0.10],[92.5,0.10],[93.5,0.10],[94.5,0.30],[95.5,0.30],[96.5,-0.00],[97.5,0.20],[98.5,0.20],[99.5,0.20],[100.5,0.20],[101.5,0.60],[102.5,0.40],[103.5,0.40],[104.5,0.20],[105.5,0.40],[106.5,0.20],[107.5,0.20],[108.5,0.20],[109.5,0.40],[110.5,0.30],[111.5,0.30],[112.5,0.30],[113.5,0.30],[114.5,0.10],[115.5,-0.20],[116.5,-0.10],[117.5,-0.10],[118.5,-0.10],[119.5,0.00],[120.5,0.00],[121.5,-0.10],[122.5,0.00],[123.5,0.10],[124.5,-0.20],[125.5,-0.20],[126.5,-0.20],[127.5,-0.50],[128.5,-0.60],[129.5,-0.20],[130.5,0.00],[131.5,0.00],[132.5,0.20],[133.5,0.30],[134.5,0.10],[135.5,0.20],[136.5,0.20],[137.5,0.20],[138.5,0.10],[139.5,0.10],[140.5,0.00],[141.5,0.00],[142.5,0.00],[143.5,0.00],[144.5,-0.20],[145.5,-0.20],[146.5,-0.20],[147.5,-0.20],[148.5,-0.40],[149.5,-0.20],[150.5,-0.20],[151.5,-0.20],[152.5,-0.20],[153.5,0.20],[154.5,0.00],[155.5,0.00],[156.5,-0.20],[157.5,-0.20],[158.5,-0.40],[159.5,-0.20],[160.5,-0.20],[161.5,0.00],[162.5,0.00],[163.5,-0.20],[164.5,0.00],[165.5,-0.20],[166.5,-0.20],[167.5,-0.20],[168.5,-0.20],[169.5,-0.40],[170.5,-0.20],[171.5,-0.20],[172.5,-0.10],[173.5,0.10],[174.5,0.10],[175.5,0.10],[176.5,0.10],[177.5,0.00],[178.5,0.00],[179.5,0.00],[180.5,0.00],[181.5,0.10],[182.5,-0.10],[183.5,0.00],[184.5,0.00],[185.5,0.00],[186.5,-0.10],[187.5,0.10],[188.5,0.00],[189.5,0.20],[190.5,0.20],[191.5,0.20],[192.5,0.20],[193.5,0.20],[194.5,0.00],[195.5,0.00],[196.5,0.00],[197.5,0.00],[198.5,0.00],[199.5,0.00],[200.5,0.00],[201.5,0.00],[202.5,0.00],[203.5,0.00],[204.5,-0.20],[205.5,-0.20],[206.5,-0.20],[207.5,-0.40],[208.5,-0.20],[209.5,0.00],[210.5,0.00],[211.5,-0.20],[212.5,0.00],[213.5,-0.20],[214.5,0.00],[215.5,0.00],[216.5,0.00],[217.5,0.00],[218.5,-0.20],[219.5,-0.40],[220.5,-0.40],[221.5,-0.20],[222.5,-0.20],[223.5,0.00],[224.5,0.00],[225.5,-0.20],[226.5,0.00],[227.5,0.00],[228.5,0.00],[229.5,0.00],[230.5,0.00],[231.5,-0.40],[232.5,-0.40],[233.5,-0.40],[234.5,-0.40],[235.5,-0.20],[236.5,0.00],[237.5,0.00],[238.5,0.00],[239.5,0.20],[240.5,0.20],[241.5,0.20],[242.5,0.20],[243.5,0.20],[244.5,0.00],[245.5,-0.20],[246.5,-0.40],[247.5,-0.40],[248.5,-0.60],[249.5,-0.60],[250.5,-0.40],[251.5,-0.40],[252.5,-0.40],[253.5,-0.20],[254.5,-0.20],[255.5,-0.20],[256.5,-0.00],[257.5,-0.00],[258.5,-0.00],[259.5,-0.00],[260.5,-0.00],[261.5,-0.20],[262.5,-0.20],[263.5,-0.20],[264.5,-0.20],[265.5,-0.20],[266.5,-0.00],[267.5,-0.00],[268.5,0.20],[269.5,0.20],[270.5,0.20],[271.5,0.20],[272.5,0.20],[273.5,-0.00],[274.5,-0.20],[275.5,-0.20],[276.5,-0.40],[277.5,-0.40],[278.5,-0.20],[279.5,0.20],[280.5,0.20],[281.5,0.60],[282.5,0.60],[283.5,0.60],[284.5,0.40],[285.5,0.40],[286.5,0.20],[287.5,0.20],[288.5,-0.00],[289.5,-0.00],[290.5,-0.00],[291.5,0.20],[292.5,0.20],[293.5,0.20],[294.5,-0.00],[295.5,-0.00],[296.5,-0.10],[297.5,-0.10],[298.5,-0.30],[299.5,-0.10],[300.5,-0.30],[301.5,-0.40],[302.5,-0.40],[303.5,-0.20],[304.5,-0.20],[305.5,0.00],[306.5,0.00],[307.5,0.00],[308.5,0.00],[309.5,-0.20],[310.5,-0.20],[311.5,-0.20],[312.5,-0.20],[313.5,-0.20],[314.5,0.00],[315.5,0.20],[316.5,0.20],[317.5,0.20],[318.5,0.30],[319.5,0.40],[320.5,0.20],[321.5,0.20],[322.5,0.20],[323.5,0.10],[324.5,0.00],[325.5,0.00],[326.5,0.00],[327.5,0.00],[328.5,-0.20],[329.5,0.00],[330.5,0.00],[331.5,-0.20],[332.5,-0.20],[333.5,0.00],[334.5,-0.20],[335.5,-0.40],[336.5,-0.20],[337.5,-0.20],[338.5,-0.20],[339.5,-0.20],[340.5,-0.20],[341.5,-0.20],[342.5,0.00],[343.5,0.00],[344.5,0.00],[345.5,0.20],[346.5,0.20],[347.5,0.20],[348.5,0.20],[349.5,0.40],[350.5,0.40],[351.5,0.40],[352.5,0.20],[353.5,0.20],[354.5,0.00],[355.5,-0.20],[356.5,-0.20],[357.5,-0.20],[358.5,-0.20],[359.5,-0.40],[360.5,-0.20],[361.5,-0.40],[362.5,-0.40],[363.5,-0.40],[364.5,-0.20],[365.5,-0.20],[366.5,0.00],[367.5,0.00],[368.5,0.20],[369.5,0.20],[370.5,0.20],[371.5,0.40],[372.5,0.40],[373.5,0.20],[374.5,0.40],[375.5,0.40],[376.5,0.40],[377.5,0.40],[378.5,0.40],[379.5,0.40],[380.5,0.40],[381.5,0.20],[382.5,0.40],[383.5,0.40],[384.5,0.20],[385.5,0.20],[386.5,0.40],[387.5,0.20],[388.5,0.20],[389.5,0.20],[390.5,0.20],[391.5,0.00],[392.5,0.20],[393.5,0.20],[394.5,0.20],[395.5,0.40],[396.5,0.40],[397.5,0.00],[398.5,0.20],[399.5,0.00],[400.5,0.00],[401.5,0.00],[402.5,0.20],[403.5,0.00],[404.5,0.20],[405.5,0.00],[406.5,0.00],[407.5,0.00],[408.5,0.00],[409.5,-0.20],[410.5,0.00],[411.5,0.00],[412.5,0.00],[413.5,0.20],[414.5,0.40],[415.5,0.20],[416.5,0.20],[417.5,0.20],[418.5,0.00],[419.5,-0.20],[420.5,-0.20],[421.5,-0.20],[422.5,-0.20],[423.5,-0.20],[424.5,0.00],[425.5,-0.20],[426.5,-0.20],[427.5,-0.20],[428.5,-0.20],[429.5,-0.40],[430.5,-0.20],[431.5,-0.20],[432.5,0.00],[433.5,0.00],[434.5,0.20],[435.5,0.20],[436.5,0.20],[437.5,-0.20],[438.5,-0.20],[439.5,-0.20],[440.5,-0.20],[441.5,-0.20],[442.5,0.00],[443.5,0.00],[444.5,0.00],[445.5,0.00],[446.5,0.20],[447.5,0.20],[448.5,0.20],[449.5,0.30],[450.5,0.30],[451.5,0.10],[452.5,0.20],[453.5,0.20],[454.5,0.20],[455.5,0.20],[456.5,0.20],[457.5,0.10],[458.5,0.10],[459.5,0.20],[460.5,0.20],[461.5,0.20],[462.5,0.00],[463.5,0.20],[464.5,0.20],[465.5,0.20],[466.5,0.20],[467.5,0.20],[468.5,0.00],[469.5,-0.40],[470.5,-0.40],[471.5,-0.40],[472.5,-0.20],[473.5,-0.20],[474.5,0.00],[475.5,0.00],[476.5,-0.20],[477.5,-0.20],[478.5,-0.20],[479.5,-0.20],[480.5,-0.20],[481.5,0.00],[482.5,0.00],[483.5,0.00],[484.5,-0.20],[485.5,-0.20],[486.5,-0.20],[487.5,-0.20],[488.5,-0.20],[489.5,0.10],[490.5,-0.10],[491.5,-0.10],[492.5,-0.10],[493.5,-0.10],[494.5,0.00],[495.5,0.00],[496.5,0.00],[497.5,0.00],[498.5,0.00],[499.5,-0.20],[500.5,0.10],[501.5,0.10],[502.5,0.10],[503.5,-0.10],[504.5,-0.10],[505.5,-0.20],[506.5,0.00],[507.5,0.00],[508.5,0.20],[509.5,0.20],[510.5,0.40],[511.5,0.20],[512.5,0.20],[513.5,0.20],[514.5,0.20],[515.5,0.00],[516.5,0.00],[517.5,0.20],[518.5,0.20],[519.5,0.20],[520.5,0.20],[521.5,0.30],[522.5,0.10],[523.5,0.20],[524.5,0.20],[525.5,0.20],[526.5,-0.10],[527.5,0.00],[528.5,-0.30],[529.5,-0.50],[530.5,-0.50],[531.5,-0.50],[532.5,-0.60],[533.5,-0.40],[534.5,0.00],[535.5,0.00],[536.5,0.20],[537.5,0.00],[538.5,0.00],[539.5,-0.20],[540.5,-0.20],[541.5,-0.20]];dis_datapoints = [];trans_datapoints = [];sec_helix_datapoints = [[9,23],[190,196],[525,532]];sec_strand_datapoints = [[59,72],[79,84],[92,96],[100,107],[134,137],[169,174],[180,183],[200,200],[201,206],[222,227],[266,267],[274,278],[282,289],[294,301],[306,311],[324,327],[332,339],[346,350],[439,445],[460,465],[499,502],[507,514],[521,524],[537,542]];acc_exposed_datapoints = [[1,4],[35,35],[37,38],[41,42],[47,47],[50,50],[53,57],[74,75],[77,78],[97,97],[127,128],[130,130],[147,148],[166,166],[210,212],[214,214],[216,217],[219,219],[221,221],[271,271],[279,281],[303,303],[341,341],[343,343],[378,378],[382,382],[390,390],[435,435],[483,483],[485,486],[516,516],[543,544]];acc_buried_datapoints = [[7,27],[30,30],[36,36],[43,43],[45,45],[49,49],[60,61],[63,63],[65,65],[68,68],[72,72],[76,76],[81,86],[88,93],[95,95],[101,111],[113,122],[131,131],[133,142],[150,150],[152,162],[169,170],[172,174],[176,176],[178,190],[192,200],[201,206],[220,220],[222,227],[231,231],[235,241],[253,253],[255,270],[272,272],[275,276],[283,296],[298,300],[302,302],[305,317],[319,320],[322,337],[339,339],[346,351],[367,368],[372,372],[388,388],[407,407],[414,415],[417,421],[430,430],[434,434],[439,439],[441,448],[450,465],[481,481],[496,496],[498,504],[508,508],[510,515],[519,528],[530,542]];flot_plot_options = []; flot_plot_options[0] = {grid: {borderWidth: {top: 0,right: 0,bottom: 0,left: 0}},legend: {show: false},xaxes: [{show: true,min: 0,max: 200,ticks: [[0.5, '1'], [24.5, '25'], [49.5, '50'], [74.5, '75'], [99.5, '100'], [124.5, '125'], [149.5, '150'], [174.5, '175'], [199.5, '200']],tickLength: -5}],yaxes: [{show: true,ticks: [[0, '0'], [4.5,'hydro-<br>phobic&nbsp;&nbsp;'], [-4.5,'hydro-<br>philic&nbsp;&nbsp;']],min: -4.5,max: +4.5,font: {size: 12,lineHeight: 14,style: 'italic',weight: 'bold',family: 'sans-serif',variant: 'small-caps',color: 'rgba(100,149,237,1)'}},{show: true,ticks: [[0, ''], [1,'positive<br>&nbsp;charge'], [-1,'negative<br>&nbsp;charge']],position: 'right',min: -1,max: 1,font: {size: 12,lineHeight: 14,style: 'italic',weight: 'bold',family: 'sans-serif',variant: 'small-caps',color: 'rgba(255,99,71,1)'}}]};number_of_plots = 3;for ( plot_num = 1 ; plot_num < number_of_plots ; plot_num ++){flot_plot_options[plot_num] = $.extend(true, {} ,flot_plot_options[0]);flot_plot_options[plot_num].xaxes = [{min: plot_num*200,max: (plot_num + 1)*200,ticks: [ [plot_num*200 +   0.5, (plot_num*200 +   1).toString()], [plot_num*200 +  24.5, (plot_num*200 +  25).toString()], [plot_num*200 +  49.5, (plot_num*200 +  50).toString()], [plot_num*200 +  74.5, (plot_num*200 +  75).toString()], [plot_num*200 +  99.5, (plot_num*200 + 100).toString()], [plot_num*200 + 124.5, (plot_num*200 + 125).toString()], [plot_num*200 + 149.5, (plot_num*200 + 150).toString()], [plot_num*200 + 174.5, (plot_num*200 + 175).toString()], [plot_num*200 + 199.5, (plot_num*200 + 200).toString()] ],tickLength: -5}];};try {if( $('#AutoAnnotator_container_1381243726671 #hydrophobicity_charge_button').val() =='Show' ){$('#AutoAnnotator_container_1381243726671 #hydrophobicity_charge_container').css('display','block');$('#AutoAnnotator_container_1381243726671 #hydrophobicity_charge_button').val('Hide');var description_html = '<div id=\'AutoAnnotator_plot_selectors\'>';description_html = description_html + '<br>&nbsp;<input type=\'checkbox\' id=\'hydrophobicity_checkbox\' checked=\'checked\'>&nbsp;Moving average over 5 amino acids for hydrophobicity (<img src=\'https://static.igem.org/mediawiki/2013/e/e9/TUM13_hydrophobicity_icon.png\' alt=\'blue graph\' height=\'10\'></img>)';description_html = description_html + '<br>&nbsp;<input type=\'checkbox\' id=\'charge_checkbox\' checked=\'checked\'>&nbsp;Moving average over 5 amino acids for charge (<img src=\'https://static.igem.org/mediawiki/2013/3/3e/TUM13_charge_icon.png\' alt=\'red graph\' height=\'10\'></img>)';description_html = description_html + '<br>&nbsp;<input type=\'checkbox\' id=\'dis_checkbox\' checked=\'checked\'>&nbsp;Predicted disulfid bridges (<img src=\'https://static.igem.org/mediawiki/2013/2/28/TUM13_dis_icon.png\' alt=\'yellow circle\' height=\'10\'></img>) with the number of the bridge in the center';description_html = description_html + '<br>&nbsp;<input type=\'checkbox\' id=\'trans_checkbox\' checked=\'checked\'>&nbsp;Predicted transmembrane helices (<img src=\'https://static.igem.org/mediawiki/2013/7/78/TUM13_trans_icon.png\' alt=\'turquois bars\' height=\'10\'></img>)';description_html = description_html + '<br>&nbsp;<input type=\'checkbox\' id=\'sec_checkbox\' checked=\'checked\'>&nbsp;Predicted secondary structure: Helices (<img src=\'https://static.igem.org/mediawiki/2013/b/bf/TUM13_helix_icon.png\' alt=\'violet bars\' height=\'10\'></img>) and beta-strands (<img src=\'https://static.igem.org/mediawiki/2013/b/bf/TUM13_strand_icon.png\' alt=\'yellow bars\' height=\'10\'></img>)';description_html = description_html + '<br>&nbsp;<input type=\'checkbox\' id=\'acc_checkbox\' checked=\'checked\'>&nbsp;Predicted solvent accessability: Exposed (<img src=\'https://static.igem.org/mediawiki/2013/1/16/TUM13_exposed_icon.png\' alt=\'blue bars\' height=\'10\'></img>) and buried (<img src=\'https://static.igem.org/mediawiki/2013/0/0b/TUM13_buried_icon.png\' alt=\'green bars\' height=\'10\'></img>) residues';description_html = description_html + '<br></div>';$('#AutoAnnotator_container_1381243726671 #hydrophobicity_charge_explanation').html(description_html);plot_according_to_selectors_1381243726671();$('#AutoAnnotator_container_1381243726671 #AutoAnnotator_plot_selectors').find('input').click(plot_according_to_selectors_1381243726671);}else{$('#AutoAnnotator_container_1381243726671 #hydrophobicity_charge_container').css('display','none');$('#AutoAnnotator_container_1381243726671 #hydrophobicity_charge_button').val('Show');$('#AutoAnnotator_container_1381243726671 #hydrophobicity_charge_explanation').html('');}}catch(err){txt='There was an error with the button controlling the visibility of the plot.\n';txt=txt+'The originating error is:\n' + err + '\n\n';alert(txt);}};function plot_according_to_selectors_1381243726671(){try{var plot_datasets = [[],[]];if($('#AutoAnnotator_container_1381243726671 #hydrophobicity_checkbox').prop('checked') == true){plot_datasets[0] = { color: 'rgba(100,149,237,1)',data: hydrophobicity_datapoints,label: 'Hydrophobicity',lines: { show: true, fill: true, fillColor: 'rgba(100,149,237,0.1)' },yaxis: 1};}if($('#AutoAnnotator_container_1381243726671 #charge_checkbox').prop('checked') == true){plot_datasets[1] = {color: 'rgba(255,99,71,1)',data: charge_datapoints,label: 'Charge',lines: { show: true, fill: true, fillColor: 'rgba(255,99,71,0.1)' },yaxis: 2};}for (plot_num = 0 ; plot_num < number_of_plots ; plot_num ++){$.plot('#AutoAnnotator_container_1381243726671 #hydrophobicity_charge_placeholder'+ plot_num.toString(), plot_datasets, flot_plot_options[plot_num] );}var screen_width = $('canvas.flot-base').width(); var pos_of_first_tick = 46;var pos_of_last_tick = screen_width - 51;var tick_diff = (screen_width - 97)/199;if($('#AutoAnnotator_container_1381243726671 #dis_checkbox').prop('checked') == true){for ( j = 0 ; j < dis_datapoints.length ; j++ ){$('#AutoAnnotator_container_1381243726671 #hydrophobicity_charge_placeholder' + Math.floor((dis_datapoints[j][0] - 1)/200) ).append('<div class=\'AutoAnnotator_dis\' style=\'left:' + ((pos_of_first_tick - 8 + (dis_datapoints[j][0] - 1)*tick_diff - Math.floor((dis_datapoints[j][0] - 1)/200)*200*tick_diff).toFixed(0)).toString() + 'px;\'><b>' + (j+1) + '</b></div>');$('#AutoAnnotator_container_1381243726671 #hydrophobicity_charge_placeholder' + Math.floor((dis_datapoints[j][1] - 1)/200) ).append('<div class=\'AutoAnnotator_dis\' style=\'left:' + ((pos_of_first_tick - 8 + (dis_datapoints[j][1] - 1)*tick_diff - Math.floor((dis_datapoints[j][1] - 1)/200)*200*tick_diff).toFixed(0)).toString() + 'px;\'><b>' + (j+1) + '</b></div>');}}if($('#AutoAnnotator_container_1381243726671 #trans_checkbox').prop('checked') == true){for ( j = 0 ; j < trans_datapoints.length ; j++ ){$('#AutoAnnotator_container_1381243726671 #hydrophobicity_charge_placeholder' + Math.floor((trans_datapoints[j][0] - 1)/200) ).append('<div class=\'AutoAnnotator_trans\' style=\'width:' + (((trans_datapoints[j][1] - trans_datapoints[j][0] + 1)*tick_diff).toFixed(0)).toString() + 'px ;left:' + ((pos_of_first_tick + (trans_datapoints[j][0] - 1.5)*tick_diff - Math.floor((trans_datapoints[j][0] - 1)/200)*200*tick_diff).toFixed(0)).toString() + 'px\'></div>');}}if($('#AutoAnnotator_container_1381243726671 #sec_checkbox').prop('checked') == true){for ( j = 0 ; j < sec_helix_datapoints.length ; j++ ){$('#AutoAnnotator_container_1381243726671 #hydrophobicity_charge_placeholder' + Math.floor((sec_helix_datapoints[j][0] - 1)/200) ).append('<div class=\'AutoAnnotator_sec_helix\' style=\'width:' + (((sec_helix_datapoints[j][1] - sec_helix_datapoints[j][0] + 1)*tick_diff).toFixed(0)).toString() + 'px; left:' + ((pos_of_first_tick + (sec_helix_datapoints[j][0] - 1.5)*tick_diff - Math.floor((sec_helix_datapoints[j][0] - 1)/200)*200*tick_diff).toFixed(0)).toString() + 'px\'></div>');}for ( j = 0 ; j < sec_strand_datapoints.length ; j++ ){$('#AutoAnnotator_container_1381243726671 #hydrophobicity_charge_placeholder' + Math.floor((sec_strand_datapoints[j][0] - 1)/200) ).append('<div class=\'AutoAnnotator_sec_strand\' style=\'width:' + (((sec_strand_datapoints[j][1] - sec_strand_datapoints[j][0] + 1)*tick_diff).toFixed(0)).toString() + 'px; left:' + ((pos_of_first_tick + (sec_strand_datapoints[j][0] - 1.5)*tick_diff - Math.floor((sec_strand_datapoints[j][0] - 1)/200)*200*tick_diff).toFixed(0)).toString() + 'px\'></div>');}}if($('#AutoAnnotator_container_1381243726671 #acc_checkbox').prop('checked') == true){for ( j = 0 ; j < acc_buried_datapoints.length ; j++ ){$('#AutoAnnotator_container_1381243726671 #hydrophobicity_charge_placeholder' + Math.floor((acc_buried_datapoints[j][0] - 1)/200) ).append('<div class=\'AutoAnnotator_acc_buried\' style=\'width:' + (((acc_buried_datapoints[j][1] - acc_buried_datapoints[j][0] + 1)*tick_diff).toFixed(0)).toString() + 'px; left:' + ((pos_of_first_tick + (acc_buried_datapoints[j][0] - 1.5)*tick_diff - Math.floor((acc_buried_datapoints[j][0] - 1)/200)*200*tick_diff).toFixed(0)).toString() + 'px\'></div>');}for ( j = 0 ; j < acc_exposed_datapoints.length ; j++ ){$('#AutoAnnotator_container_1381243726671 #hydrophobicity_charge_placeholder' + Math.floor((acc_exposed_datapoints[j][0] - 1)/200) ).append('<div class=\'AutoAnnotator_acc_exposed\' style=\'width:' + (((acc_exposed_datapoints[j][1] - acc_exposed_datapoints[j][0] + 1)*tick_diff).toFixed(0)).toString() + 'px; left:' + ((pos_of_first_tick + (acc_exposed_datapoints[j][0] - 1.5)*tick_diff - Math.floor((acc_exposed_datapoints[j][0] - 1)/200)*200*tick_diff).toFixed(0)).toString() + 'px\'></div>');}}}catch(err){txt='There was an error while drawing the selected elements for the plot.\n';txt=txt+'The originating error is:\n' + err + '\n\n';throw(txt);}}</script></html>
 <!-- Uncomment this to enable Functional Parameter display