Difference between revisions of "Part:BBa K1202102:Experience"
 
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Our nanofactory includes antibody, Protein G and enzyme complex. We needed connection enzyme system between antibody as a linker.Herewith we used protein G which derived from group C and G Streptococcus strains and binds to the Fc region of numerous immunogloblulins. Protein G facilitates binding of enzyme construct to the Fc region of the targeting antibody.Also we was cloned into pSB1C3 and we express this protein in BL21 strain of E. coli. Futhermore we tagged his6x at N terminus of protein G for purificaiton.
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Our nanofactory includes antibody, Protein G and enzyme complex. We needed connection enzyme system between antibody as a linker.Herewith we used protein G which derived from group C and G Streptococcus strains and binds to the Fc region of numerous immunogloblulins. Protein G facilitates binding of enzyme construct to the Fc region of the targeting antibody.Also we was cloned into pSB1C3 and we express this protein in BL21 strain of E. coli. Futhermore we tagged his6x at N terminus of protein G for purificaiton.
  
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There is natural affinity of protein G to bind to antibody in phosphate buffer. So we incubated protein G and antibodies together. Then we measured.
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There is natural affinity of protein G to bind to antibody in phosphate buffer. So we incubated protein G and antibodies together. Then we measured.
 
Result;
 
Result;
 
[[File:Proteing.png]]
 
[[File:Proteing.png]]

Latest revision as of 07:59, 8 October 2013

Our nanofactory includes antibody, Protein G and enzyme complex. We needed connection enzyme system between antibody as a linker.Herewith we used protein G which derived from group C and G Streptococcus strains and binds to the Fc region of numerous immunogloblulins. Protein G facilitates binding of enzyme construct to the Fc region of the targeting antibody.Also we was cloned into pSB1C3 and we express this protein in BL21 strain of E. coli. Futhermore we tagged his6x at N terminus of protein G for purificaiton.

There is natural affinity of protein G to bind to antibody in phosphate buffer. So we incubated protein G and antibodies together. Then we measured. Result; Proteing.png Our protein G weight is 25 kDas and our combined Protein G + antibody protein’s weight is about 50 kDas. We accepted the experiment result that protein G nearby of 25 kDas line and the complex nearby of 50 kDas. The experiment throughput was the same as we accepted as you see above. The result show that we have produced nanofactory for definite.


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