Difference between revisions of "Tutorial:Adding a BioBrick Composite Part"

 
Line 5: Line 5:
 
https://static.igem.org/mediawiki/parts/5/50/Addtutorial.png
 
https://static.igem.org/mediawiki/parts/5/50/Addtutorial.png
 
|
 
|
−
Part creation is pretty straightforward if you have some basic information about your part and understand a little  about the Registry's mechanics.
+
Part creation is pretty straightforward if you have some basic information about your composite part and understand a little  about the Registry's mechanics.
  
−
In this tutorial you will be uploading a DNA sequence to your Registry [[Help:Sandbox Overview|sandbox]], annotating this sequence, and adding references and other descriptive information.  For example, the DNA sequence might be a coding region for your gene of interest, eg. ''genX'', or a sequence for a promoter, etc. In this tutorial, we take the malE gene sequence which codes the Maltose-binding Protein from e.coli.
+
In this tutorial you will be uploading a composite part to your Registry [[Help:Sandbox Overview|sandbox]], annotating this composition/device, and adding references and other descriptive information. In this tutorial, we take the malE gene BioBrick created in the [[Tutorial:Adding a Basic Part|basic tutorial]] and create a '''MBP Output Module''', by coupling the orginal BioBrick with a standard rbs and terminator from the registry. The end result is a module which takes a standard PoPs input and can initiate chemotaxis and maltose-transport when utilised in an appropriate chassis.
 
|}
 
|}
  
 
Before you add your BioBrick to your [[Help:Sandbox Overview|sandbox]], you'll need to collect the following information:
 
Before you add your BioBrick to your [[Help:Sandbox Overview|sandbox]], you'll need to collect the following information:
−
<div style="width: 600px; padding: 5px; background-color: #ffffff; border: 2px solid #993300;">
+
<div style="width: 700px; padding: 5px; background-color: #ffffff; border: 2px solid #993300;">
 
{|
 
{|
−
|valign='top' width=300px|
+
|valign='top' width=350px|
−
* A part name
+
* A composite part/device name
−
* The DNA sequence of the part you are making
+
* The BioBrick subparts names of the device yo wish to construct
−
|valign='top' width=300px|
+
|valign='top' width=350px|
−
* A short description of the part
+
* A short description of the composite part/device
−
* A long description of the part, including references
+
* A long description of the composite part/device, including references
 
|}
 
|}
 
</div>
 
</div>
−
Remember, this information is ultimately not just for you - while at first you will be using the part's pages to document, keep a record and share information with your group, eventually you'll want to [[Help:Sandbox Overview|release your part]] to the Registry.  To this end, you should think about who your pages are relevant to initially and adapt them throughout the development process. Eventually, you should look to include the information anyone that wants to use the part will need and want access to.  The better you make your parts record, the more likely your part will be used in [[Help:BioBrick Assembly|assemblies]].
+
Remember, this information is ultimately not just for you - while at first you will be using the part's pages to document, keep a record and share information with your group, eventually you'll want to [[Help:Sandbox Overview|release your part]] to the Registry.  To this end, you should think about who your pages are relevant to initially and adapt them throughout the development process. Eventually, you should look to include the information anyone that wants to use the part will need and want access to.  The better you make your part's record, the more likely your part will be used in [[Help:BioBrick Assembly|assemblies]].
  
−
The Registry will create a parts record - it will check your sequence for unpermissable [[Help:Restriction enzymes|restriction sites]] that are incompatible with [[Help:BioBrick Prefix and Suffix|BioBrick ends]].  '''Note:'''  The Registry won't display your sequence with [[Help:BioBrick Prefix and Suffix|BioBrick ends]] unless you ask for this, eg. to get the sequence synthesized.  It will also calculate some statistics, etc.
+
The following pages provide a step-by-step guide to adding a composite part to your [[Help:Sandbox Overview|sandbox]]:
−
 
+
−
The following pages provide a step-by-step guide to adding a basic part to your [[Help:Sandbox Overview|sandbox]]:
+
  
 
== Step 1:  Registry Account ==  
 
== Step 1:  Registry Account ==  
 
You will need a [https://parts.igem.org/wiki/index.php/Help:Registry_Accounts registry account] if you don't have one.
 
You will need a [https://parts.igem.org/wiki/index.php/Help:Registry_Accounts registry account] if you don't have one.
 +
 +
It is recomemended you become familiar with adding a basic part by completing the [[Tutorial:Adding a Basic Part|tutorial]], even for a fictional BioBrick.
 +
 
<div style="width: 600px; padding: 5px; background-color: #ffffff; border: 2px solid #993300;">
 
<div style="width: 600px; padding: 5px; background-color: #ffffff; border: 2px solid #993300;">
 
<big>Once you have an account [[Tutorial:Adding a BioBrick Composite Part - Step 2|'''click here''']] to start the Tutorial proper</big>
 
<big>Once you have an account [[Tutorial:Adding a BioBrick Composite Part - Step 2|'''click here''']] to start the Tutorial proper</big>
 
</div>
 
</div>
 
|}
 
|}

Revision as of 22:06, 8 August 2006

Addtutorial.png

Part creation is pretty straightforward if you have some basic information about your composite part and understand a little about the Registry's mechanics.

In this tutorial you will be uploading a composite part to your Registry sandbox, annotating this composition/device, and adding references and other descriptive information. In this tutorial, we take the malE gene BioBrick created in the basic tutorial and create a MBP Output Module, by coupling the orginal BioBrick with a standard rbs and terminator from the registry. The end result is a module which takes a standard PoPs input and can initiate chemotaxis and maltose-transport when utilised in an appropriate chassis.

Before you add your BioBrick to your sandbox, you'll need to collect the following information:

  • A composite part/device name
  • The BioBrick subparts names of the device yo wish to construct
  • A short description of the composite part/device
  • A long description of the composite part/device, including references

Remember, this information is ultimately not just for you - while at first you will be using the part's pages to document, keep a record and share information with your group, eventually you'll want to release your part to the Registry. To this end, you should think about who your pages are relevant to initially and adapt them throughout the development process. Eventually, you should look to include the information anyone that wants to use the part will need and want access to. The better you make your part's record, the more likely your part will be used in assemblies.

The following pages provide a step-by-step guide to adding a composite part to your sandbox:

Step 1: Registry Account

You will need a registry account if you don't have one.

It is recomemended you become familiar with adding a basic part by completing the tutorial, even for a fictional BioBrick.

Once you have an account click here to start the Tutorial proper