Difference between revisions of "Part:BBa K1144016:Design"
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===Design Notes===
 
===Design Notes===
  
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HoxN is one of the most efficient nickel transporter protein which places in the membrane and allows the metal to gets into the cell [1]. On the other hand, rfp is a fluorescent protein widely used to measure gene expression.  
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HoxN is one of the most efficient nickel transporter protein which places in the membrane and allows the metal to gets into the cell [1]. On the other hand, ''rfp'' is a fluorescent protein widely used to measure gene expression. This part is an intermediate part of our design which can be fused with a promoter and then evaluate ''hoxn'' expression under the selected promoter by measuring red fluorescence.  
  
  

Revision as of 21:51, 5 October 2013

hoxN,RFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 475
    Illegal NgoMIV site found at 795
    Illegal AgeI site found at 1392
    Illegal AgeI site found at 1504
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

HoxN is one of the most efficient nickel transporter protein which places in the membrane and allows the metal to gets into the cell [1]. On the other hand, rfp is a fluorescent protein widely used to measure gene expression. This part is an intermediate part of our design which can be fused with a promoter and then evaluate hoxn expression under the selected promoter by measuring red fluorescence.



Source

hoxn is from Cupradrividus metallidurans and rfp is from iGEM plate.

References

Eitinger, T. Microbial Nickel Transport In Microbial Transport Systems. Ed. G. Winkelmann. Wiley-VCH 2001