Difference between revisions of "Part:BBa K1076004"
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| − | FadD ligates CoA in fatty acid chain preparing it to get into the Beta-Oxidation metabolic pathway. The idea is super express this protein in order to produce more ATP and H+. | + | FadD ligates CoA in fatty acid chain preparing it to get into the Beta-Oxidation metabolic pathway. The idea is super express this protein by expressing this full lenght gene, with the promoter, rbs, gene and terminator from the proper bacteria, in order in order to produce more ATP and H+. |
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Latest revision as of 21:05, 5 October 2013
FadD, Long-chain-fatty-acid-CoA ligase
FadD ligates CoA in fatty acid chain preparing it to get into the Beta-Oxidation metabolic pathway. The idea is super express this protein by expressing this full lenght gene, with the promoter, rbs, gene and terminator from the proper bacteria, in order in order to produce more ATP and H+.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1729
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1883
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1934
Illegal NgoMIV site found at 1944
Illegal AgeI site found at 1462 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 415