Difference between revisions of "Part:BBa K1104300:Design"

(→‎Design Notes)
(→‎Design Notes)
 
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===Design Notes===
 
===Design Notes===
−
   
+
==''Abaecin primers(example)''==
−
  >>ATGTATGTACCATTACCAAATGTACCACAACCAGGACGTCGTCCGTTTCCAACGTTTCCTGGTCAGGGACCATTCAATCCGAAAATTAAATGGCCTCAAGGCTATTAA (108nt)
+
  mature peptide
−
  ATGTATGTACCATTACCAAATGTACCAC (36nt)
+
  ATGTATGTACCATTACCAAATGTACCACAACCAGGACGTCGTCCGTTTCCAACGTTTCCTGGTCAGGGACCATTCAATCCGAAAATTAAATGGCCTCAAGGCTATTAA (108nt)
−
      ATGTACCATTACCAAATGTACCACAACCAGGACGTCGTCCGTTTCCAACGTTTCCTGGT (59nt)
+
ATGTATGTACCATTACCAAATGTACCAC (36nt)
−
                                              GTTTCCAACGTTTCCTGGTCAGGGACCATTCAATCCGAAAATTAAATGGCCTCAAGGCT (59nt)
+
    ATGTACCATTACCAAATGTACCACAACCAGGACGTCGTCCGTTTCCAACGTTTCCTGGT (59nt)
−
                                                                                      AATTAAATGGCCTCAAGGCTATTAA tactagtagcggccgctgcagtcc (49nt)
+
                                            GTTTCCAACGTTTCCTGGTCAGGGACCATTCAATCCGAAAATTAAATGGCCTCAAGGCT (59nt)
−
              24nt,55C                              19nt,54C                                20nt,54C
+
                                                                                    AATTAAATGGCCTCAAGGCTATTAA tactagtagcggccgctgcagtcc (49nt)
 +
            24nt,55C                              19nt,54C                                20nt,54C
  
 
*See more:[ http://www.ncbi.nlm.nih.gov/nuccore/NM_001011617.1]、[ http://www.ncbi.nlm.nih.gov/gene?cmd=Retrieve&dopt=full_report&list_uids=406144]
 
*See more:[ http://www.ncbi.nlm.nih.gov/nuccore/NM_001011617.1]、[ http://www.ncbi.nlm.nih.gov/gene?cmd=Retrieve&dopt=full_report&list_uids=406144]

Latest revision as of 08:02, 5 October 2013


Abaecin


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Abaecin primers(example)

mature peptide
ATGTATGTACCATTACCAAATGTACCACAACCAGGACGTCGTCCGTTTCCAACGTTTCCTGGTCAGGGACCATTCAATCCGAAAATTAAATGGCCTCAAGGCTATTAA (108nt)
ATGTATGTACCATTACCAAATGTACCAC (36nt)
    ATGTACCATTACCAAATGTACCACAACCAGGACGTCGTCCGTTTCCAACGTTTCCTGGT (59nt)
                                            GTTTCCAACGTTTCCTGGTCAGGGACCATTCAATCCGAAAATTAAATGGCCTCAAGGCT (59nt)
                                                                                   AATTAAATGGCCTCAAGGCTATTAA tactagtagcggccgctgcagtcc (49nt)
           24nt,55C                               19nt,54C                                20nt,54C
  • See more:[ http://www.ncbi.nlm.nih.gov/nuccore/NM_001011617.1]、[ http://www.ncbi.nlm.nih.gov/gene?cmd=Retrieve&dopt=full_report&list_uids=406144]

Source

Apis melliferra

References