Difference between revisions of "Part:BBa K1104301"
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Different from well-studied Defensins secreted from human, our Defensin1 from honey bee has many unclear properties. Even so, its effectiveness against microbes has been proved through some stable papers.</p> | Different from well-studied Defensins secreted from human, our Defensin1 from honey bee has many unclear properties. Even so, its effectiveness against microbes has been proved through some stable papers.</p> | ||
<p> Importantly, the sequence here is the mature peptide sequence because there are some introns which are barriers for ''E. coli'' to transcript genes in the genome DNA. That is to say, it is better to synthesize the gene through designing primers(about two sets >> four primers) as modules for real PCR. Comparatively, purifying genes & proteins from bees' genome DNA extraction is a more complicated and time-consuming method.</p> | <p> Importantly, the sequence here is the mature peptide sequence because there are some introns which are barriers for ''E. coli'' to transcript genes in the genome DNA. That is to say, it is better to synthesize the gene through designing primers(about two sets >> four primers) as modules for real PCR. Comparatively, purifying genes & proteins from bees' genome DNA extraction is a more complicated and time-consuming method.</p> | ||
| − | + | ==Working Mechanism== | |
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Revision as of 05:55, 5 October 2013
Defensin1
Defensin is a grand group of antimicrobial peptides, having numerous kinds of similar AMPs. They are labeled by numbers, from species to species, as their identification. Different from well-studied Defensins secreted from human, our Defensin1 from honey bee has many unclear properties. Even so, its effectiveness against microbes has been proved through some stable papers.
Importantly, the sequence here is the mature peptide sequence because there are some introns which are barriers for E. coli to transcript genes in the genome DNA. That is to say, it is better to synthesize the gene through designing primers(about two sets >> four primers) as modules for real PCR. Comparatively, purifying genes & proteins from bees' genome DNA extraction is a more complicated and time-consuming method.
Working Mechanism
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 135
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]