Difference between revisions of "Part:BBa K950002"

Revision as of 03:54, 5 October 2013

yeast anb1 promotor

There are many genes in yeast that are regulated by oxygen supply as a means to adapt to fluctuations in oxygen tension (Lowry and Zitomer, 1988). One of these genes is the ANB1 gene: under aerobic conditions the ANB1 gene is regulated by heme while it is expressed under anaerobic conditions (Lowry and Zitomer, 1984). Under aerobic conditions “the ROX1 gene product mediates the heme regulation of the ANB1 gene” (Lowry and Zitomer, 1988). Lowry and Zitomer (1988) found that this repression is regulated by the synthesis and elimination of ROX1 transcripts depending on the presence of oxygen whereby aerobic conditions induce the synthesis of heme which in turn stimulates the expression of ROX1. “ROX1 is a member of the HMG family of DNA-binding proteins” (Balasubramanian et al., 1993) and binds to a “12-bp consensus sequence repeated twice in two of the ROX1-responsive operators of ANB1” (Lowry et al., 1990). According to Lowry et al. (1990) the operator sequences (which are the targets for ROX1) are located in the upstream region of the ANB1 gene.

Short: This is the promoter of yeast ANB1 gene. Panb1 is a target of the ROX1 repressor, see Part BBa_K950001.

Increased promoter activity of Psuc2 and Panb1 in repressor deficient strains. Fluorescence was detected using a RFP filter set (ET Bandpass 470/40, ET Bandpass 572/35).

Promoter activity of Psuc2 and Panb1 in repressor deficient strains. The promoter activity was enhanced by a factor of 2.6 for Psuc2 and 13.8 for Panb1. Fluorescence was measured using a plate reader (Ex: 548nm +/-9nm, Em: 581nm +/-20nm) and normalized to OD600 of the cell suspension. All measurements were performed as triplicates.

Sequence and Features