Difference between revisions of "Part:BBa K1216000:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | No illegal restriction sites within the part | + | No illegal restriction sites within the part<br><br> |
We obtain the plasmid from Johannes Haerle of the Panke Group from D-BSSE in Basel and sequence them for restriction sites for further cloning steps | We obtain the plasmid from Johannes Haerle of the Panke Group from D-BSSE in Basel and sequence them for restriction sites for further cloning steps | ||
Revision as of 03:42, 5 October 2013
β-Glucuronidase (gusA) from Bacillis Subtilis
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 538
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
No illegal restriction sites within the part
We obtain the plasmid from Johannes Haerle of the Panke Group from D-BSSE in Basel and sequence them for restriction sites for further cloning steps
Source
Bacillus subtilis.