Difference between revisions of "Part:BBa K1197008"

Revision as of 03:05, 5 October 2013

Pveg (K823003) + RBS (SpoVG) + RFP + DT

This part is used together with K119009 and K1197010 to compare two Pveg promoter (K143012 and K823003) and one pHyperspank promoter (K606040) which used in B.subtilis and have different sequences. By RFP production and modelling data, strength of promoters were compared.

We did alignment by using CLUSTAL W between two Pveg promoters; K143012 and K823003. The result is as follows:

http://wiki.metuigem.com/images/1375868_10202188366932242_1731194941_n.jpg

K823003 sequence has 237 bp and K143012 sequence has 97 bp as seen in the figure. We can conclude that K823003 includes K143012. After BLASTing the remaining part of the K823003 which is not same as K143012 there were no results for specific sequences for polimerase binding. This shows K823003 has alike promoter strength as K143012. Only the primers were desinged further from the main promoter region. So for this promoter we can use the promoter strength of the K143012.

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Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 818
Illegal AgeI site found at 930
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 This part is used together with K119009 and K1197010 to compare two Pveg promoter (K143012 and K823003) and one pHyperspank promoter (K606040) which used in B.subtilis and have different sequences. By RFP production and modelling data, strength of promoters were compared.
+We did alignment by using CLUSTAL W between two Pveg promoters; K143012 and K823003. The result is as follows:
+http://wiki.metuigem.com/images/1375868_10202188366932242_1731194941_n.jpg
+K823003 sequence has 237 bp and K143012 sequence has 97 bp as seen in the figure. We can conclude that K823003 includes K143012. After BLASTing the remaining part of the K823003 which is not same as K143012 there were no results for specific sequences for polimerase binding. This shows K823003 has alike promoter strength as K143012. Only the primers were desinged further from the main promoter region. So for this promoter we can use the promoter strength of the K143012.
 .