Difference between revisions of "Part:BBa K1152013"

Line 18: Line 18:
 
<!-- -->
 
<!-- -->
  
 
+
<a class="fancybox fancyGraphical" href="https://static.igem.org/mediawiki/2013/c/cf/Heidelberg_IndPD_Fig6.png">
<" href="https://static.igem.org/mediawiki/2013/6/6c/Heidelberg_IndPD_Fig3.png">
+

Revision as of 02:54, 5 October 2013

IndC Indigoidine Synthetase device


This part encodes the non-ribosomal peptide synthetase that synthesizes the blue pigment indigoidine. The IndC gene was amplified from P. luminescens laumondii TT01 (DSM15139) and cloned into pSB1C3.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 4087
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1467
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 2730

Restriction digest of BBa_K1152013 with EcoRI, XbaI, SpeI and PstI


<a class="fancybox fancyGraphical" href="Heidelberg_IndPD_Fig6.png">