Difference between revisions of "Part:BBa K1152013"

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Nonribosomal Peptide Synthetase that synthesizes the blue pigment indigoidine. The gene was amplified from ''P. luminescens laumondii'' TT01 (DSM15139).
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This part encodes the non-ribosomal peptide synthetase that synthesizes the blue pigment indigoidine. The IndC gene was amplified from ''P. luminescens laumondii'' TT01 (DSM15139) and cloned into pSB1C3.
  
 
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<partinfo>BBa_K1152013 parameters</partinfo>
 
<partinfo>BBa_K1152013 parameters</partinfo>
 
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<" href="https://static.igem.org/mediawiki/2013/6/6c/Heidelberg_IndPD_Fig3.png">

Revision as of 02:52, 5 October 2013

IndC Indigoidine Synthetase device


This part encodes the non-ribosomal peptide synthetase that synthesizes the blue pigment indigoidine. The IndC gene was amplified from P. luminescens laumondii TT01 (DSM15139) and cloned into pSB1C3.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 4087
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1467
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 2730

Restriction digest of BBa_K1152013 with EcoRI, XbaI, SpeI and PstI


<" href="Heidelberg_IndPD_Fig3.png">