Difference between revisions of "Part:BBa K1150045"

Latest revision as of 02:35, 5 October 2013

uniCAS RNaimer multiple targets to VEGF 4 and bla target 2

uniCAS RNaimer multiple targets to Bla target 2 and VEGF target 4
Function	twofold targeting with one plasmid
Use in	Mammalian cells
RFC standard	RFC 25
Backbone	pSB1C3
Submitted by	[http://2013.igem.org/Team:Freiburg Freiburg 2013]

Usage and Biology

Fusion of VEGF-target 4 and bla-target 2. This RNAimer was build via iGEM-ligation method and contains the DNA-target sequences of one areas of the bla-gene and one area of the VEGF locus. This enables multiple gene targeting throught one plasmid. The transfection with such a multiple target-plasmid avoids additional cell stress compared to transfection with serveral single target-plasmids.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 224
Illegal BglII site found at 1061
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 216
Illegal BsaI.rc site found at 1053

@@ Line 21: / Line 21: @@
 |}
-===Usage and Biology===
+==Usage and Biology==
 Fusion of [https://parts.igem.org/Part:BBa_K1150040 VEGF-target 4] and [https://parts.igem.org/Part:BBa_K1150040 bla-target 2]. This RNAimer was build via iGEM-ligation method and contains the DNA-target sequences of one areas of the bla-gene and one area of the VEGF locus. This enables multiple gene targeting throught one plasmid. The transfection with such a multiple target-plasmid avoids additional cell stress compared to transfection with serveral single target-plasmids.