Difference between revisions of "Part:BBa K1155007"
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lacZ gene encodes the protein beta-galactosidase which causes bacteria expressing the gene to appear blue when grown on a medium that contains the substrate analog X-gal. | lacZ gene encodes the protein beta-galactosidase which causes bacteria expressing the gene to appear blue when grown on a medium that contains the substrate analog X-gal. | ||
| − | In order to be easily used by all the other teams, our team improved the biobrick BBa_I732017 designed by iGEM07_USTC by asembly of biobrick BBa_I732017 and B0017. | + | In order to be easily used by all the other teams, our team improved the biobrick [https://parts.igem.org/Part:BBa_I732017 BBa_I732017] designed by iGEM07_USTC by asembly of biobrick BBa_I732017 and B0017. |
A composite part constituted of a RBS, full length lacZ gene and a terminator. We used it as one of our reporter gene in ''Escherichia coli''. | A composite part constituted of a RBS, full length lacZ gene and a terminator. We used it as one of our reporter gene in ''Escherichia coli''. | ||
Revision as of 02:34, 5 October 2013
RBS-lacZ-term
lacZ gene encodes the protein beta-galactosidase which causes bacteria expressing the gene to appear blue when grown on a medium that contains the substrate analog X-gal.
In order to be easily used by all the other teams, our team improved the biobrick BBa_I732017 designed by iGEM07_USTC by asembly of biobrick BBa_I732017 and B0017.
A composite part constituted of a RBS, full length lacZ gene and a terminator. We used it as one of our reporter gene in Escherichia coli.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]