Difference between revisions of "Part:BBa K1152013"
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===Usage and Biology=== | ===Usage and Biology=== | ||
| − | + | The single module NRPS indC from ''P. luminescens'' contains an internal oxidation domain (Ox-domain) in its A-domain and a special TE-domain . This enzyme first adenylates L-glutamine (A-domain), which is then attached to the T-domain. The TE-domain cleaves and catalyzes the cyclization of the substrate, which is further oxidized by the Ox-domain. The oxidation of two cyclic glutamines results in the formation of the insoluble blue pigment indigoidine. <br/> | |
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
Revision as of 02:32, 5 October 2013
IndC Indigoidine Synthetase device
Nonribosomal Peptide Synthetase that synthesizes the blue pigment indigoidine. The gene was amplified from P. luminescens laumondii TT01 (DSM15139).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 4087
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1467
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 2730
