Difference between revisions of "Part:BBa K1197006"

Revision as of 02:06, 5 October 2013

MazF and LacI expression construct

MazF is a non-specific ribonuclease coding gene is used as killing agent against Bacillus subtilis. In our system, MazF is produced constitutively under Pveg promoter.

When MazF construct (Pveg + RBS SpoVG + MazF + RBS SpoVG + LacI + DT) was assemled in B.subtilis integration vector, BBa_K823023, we saw colonies when it is transformed into E.coli with Ampicillin resistance. On the other hand, we did not see any colony when the same plasmid was transformed into B.subtilis. Thus, it can be said that MazF construct is working in B.subtilis functionally.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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+MazF is a non-specific ribonuclease coding gene is used as killing agent against Bacillus subtilis. In our system, MazF is produced constitutively under Pveg promoter.
+When MazF construct (Pveg + RBS SpoVG + MazF + RBS SpoVG + LacI + DT) was assemled in B.subtilis integration vector, BBa_K823023, we saw colonies when it is transformed into E.coli with Ampicillin resistance. On the other hand, we did not see any colony when the same plasmid was transformed into B.subtilis. Thus, it can be said that MazF construct is working in B.subtilis functionally.
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