Difference between revisions of "Part:BBa K1075049"

Latest revision as of 02:05, 5 October 2013

AraC-pBAD-RBS32-sspB-TT-pLac-RBS34-mCherry-TT

This part contains sspB under the control of the AraC promoter and mCherry under the control of the pLac promoter.

Usage and Biology

The sspB protein is an adaptor responsible for delivering ssrA-tagged substrates to the ClpXP protease in order to enhance their degradation.

mCherry is a red fluorescent protein with the excitation maximum at 587 nm and the Emission maximum at 610 nm. [http://www.ncbi.nlm.nih.gov/pubmed/15558047]

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 1234
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 1069
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI site found at 1051

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K1075049 short</partinfo>
-a
+This part contains sspB under the control of the AraC promoter and mCherry under the control of the pLac promoter.
-<!-- Add more about the biology of this part here
 ===Usage and Biology===
+The sspB protein is an adaptor responsible for delivering ssrA-tagged substrates to the ClpXP protease in order to enhance their degradation.
+mCherry is a red fluorescent protein with the excitation maximum at 587 nm and the Emission maximum  at 610 nm. [http://www.ncbi.nlm.nih.gov/pubmed/15558047]
 <!-- -->