Difference between revisions of "Part:BBa K1075028"

Latest revision as of 01:33, 5 October 2013

SplitSspB(Rapamycin inducable)-pLac-RBS34-mCherry-TT

The part contains the promoter pLac, a ribosomal binding site, the red fluorescent protein mCherry and a double terminator. It is integrated in the plasmid pJD427, which contains a ecSspB split system.

biology

The plasmid pJD427 contains the two parts of split ecSspB, which can be fused (and thus regain function) by addition of rapamycin. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3220803/

plac is a IPTG inducible promoter.

The ribosomal binding side is a sequence of mRNA where the ribosome binds to start translation.

mCherry is a red fluorescent protein with the excitation maximum at 587 nm and the emission maximum at 610 nm. http://www.ncbi.nlm.nih.gov/pubmed/15558047

The double terminator stops the transcription at this point.

application

This plasmid is a negative control to the pJD427-pLac2-RBS34-mCherry-ecssrA(DAS+4)-TT plasmid. [[1]]

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 1174
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 102
Illegal BsaI.rc site found at 743

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K1075028 short</partinfo>
+The part contains the promoter pLac, a ribosomal binding site, the red fluorescent protein mCherry and a double terminator. It is integrated in the plasmid pJD427, which contains a ecSspB split system.
-<!-- Add more about the biology of this part here-->
-===construction===
-The part contains the promoter plac, a ribosomal binding site, the red fluorescent protein mCherry and a double terminator. It is integrated in the plasmid pJD427, which contains a ecSspB split system.
 ===biology===
-The plasmid pJD427 contains the two parts of splitted ecSspB, which can be fused (and thus regain function) by addition of rapamycin. [[http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3220803/]]
+The plasmid pJD427 contains the two parts of split ecSspB, which can be fused (and thus regain function) by addition of rapamycin. [[http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3220803/]]