Difference between revisions of "Part:BBa K1163102"

Revision as of 00:13, 5 October 2013

pAceB-sfGFP-Term
iGEM Evry 2013

Description:
This part is composed of the following elements:

• AceB promoter region
• superfolder GFP
• Terminator

The AceB gene encodes the malate synthase A enzyme, which is involved in carbon source management. Its promoter region has been extracted from the genome of Escherichia coli. We found after genomic research that the gene is under the regulation of fur, thus meaning it is sensitive to iron concentration variations.

FUR (Ferric Uptake Regulation) is a transcriptional repressor of genes involved in iron homeostasis. In the presence of iron, FUR binds iron and dimerizes. This modification of conformation allows the linkage to a Fur Binding Site and inhibits the mRNA transcription of the downstream gene.
This 300 bp long promoter sequence contains a RBS and a FUR binding site, although it has not been clearly possible to identify them.

Characterization:
We characterized its inhibition ability by cloning a superfolder GFP downstream at a iron concentration range from 10^-7 to 10^-4 mol.L^-1.

Compatibility
Chassis: Device has been shown to work in BBa_V1004
Plasmids: Device has been shown to work on pSB1A3
Devices: Device has been shown to work with BBa_K1163102, BBa_K1163103

Why would you use this part?
Sequence and Features