Difference between revisions of "Part:BBa K1202103"

 
 
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__NOTOC__
 
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<partinfo>BBa_K1202103 short</partinfo>
 
<partinfo>BBa_K1202103 short</partinfo>
  
  
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E.coli  secrete this protein , this protein get into the all cells but only force cancer cells to apoptosis
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Our cancer killing system, TAT-Apoptin, must be secreted from our bacteria. For this purpose, we decided to add HlyA signal peptide in the downstream region of our part. HlyA works only if it is placed in the N-terminus of protein. With this design, our TAT-Apoptin system can go out from bacteria.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 00:10, 5 October 2013

Tat-Apoptin with HlyA signal peptide


Our cancer killing system, TAT-Apoptin, must be secreted from our bacteria. For this purpose, we decided to add HlyA signal peptide in the downstream region of our part. HlyA works only if it is placed in the N-terminus of protein. With this design, our TAT-Apoptin system can go out from bacteria.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 348
  • 1000
    COMPATIBLE WITH RFC[1000]