Difference between revisions of "Part:BBa K1202102"

Latest revision as of 00:05, 5 October 2013

Nanofactory Connecting Domain (Protein G)

Protein G is used as a protein linker domain and antibody Fc section binding tag in literature. In our design, NanoFactory consists of two main sections: Binding and enzyme zones. By linking these two sections with Protein G, we hope to conduct excellent protein folding and less complication in our experiments. Additionally, we want to use the Fc binding ability of Protein G for the alternative NanoFactory binding zone construction technique.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 93

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K1202102 short</partinfo>
-Protein G has a special area that binds to antibody's Fc fragment.
+Protein G is used as a protein linker domain and antibody Fc section binding tag in literature. In our design, NanoFactory consists of two main sections: Binding and enzyme zones. By linking these two sections with Protein G, we hope to conduct excellent protein folding and less complication in our experiments. Additionally, we want to use the Fc binding ability of Protein G for the alternative NanoFactory binding zone construction technique.
 <!-- Add more about the biology of this part here