Difference between revisions of "Part:BBa K1163101:Design"

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===Design Notes===
 
===Design Notes===
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We blindly extracted the 300 bp sequence upstream of the AceB gene in E. coli. It contains a putative FUR binding site and a putative RBS.
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We extracted the 300 bp sequence upstream of the AceB gene in Escherichia coli. It contains a putative FUR binding site and a putative RBS.
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===Source===
 
===Source===

Revision as of 23:50, 4 October 2013


pAceB promoter region


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 277
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We extracted the 300 bp sequence upstream of the AceB gene in Escherichia coli. It contains a putative FUR binding site and a putative RBS.

Source

Genomic DNA.

References

Functional Interactions between the Carbon and Iron Utilization Regulators, Crp and Fur, in Escherichia coli. J. Bacteriol. February 2005 vol. 187 no. 3 980-990