Difference between revisions of "Part:BBa K1163102"

Revision as of 23:26, 4 October 2013

pAceB-sfGFP-Term
iGEM Evry 2013

Description:
This part is composed of the following elements:

• AceB promoter region
• superfolder GFP
• Terminator

The AceB gene encodes the malate synthase A enzyme, which is involved in carbon source management. Its promoter region has been extracted from the genome of Escherichia coli. We found after genomic research that the gene is under the regulation of fur, thus meaning it is sensitive to iron concentration variations.

FUR (Ferric Uptake Regulation) is a transcriptional repressor of genes involved in iron homeostasis. In the presence of iron, FUR binds iron and dimerizes. This modification of conformation allows the linkage to a Fur Binding Site and inhibits the mRNA transcription of the downstream gene.
This 300 bp long promoter sequence contains a RBS and a FUR binding site, although it has not been clearly possible to identify them.

Characterization
We characterized its inhibition ability by cloning a superfolder GFP downstream at a iron concentration range from 10^-7 to 10^-4 mol.L^-1. Why would you use this part? This part allows the characterization of the AceB promoter region thanks to the sfGFP downstream. Sequence and Features