Difference between revisions of "Part:BBa K1150040"

(Usage and Biology)
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====Usage and Biology====
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===Usage and Biology===
 
This device contains loci coding for small RNAs that are able to target the catalytically-inactive, engineered [https://parts.igem.org/Part:BBa_K1150000 dCas9] protein to the Bla locus target 2 .  This RNaimer is bulid of the uniCAS [https://parts.igem.org/Part:BBa_K1150034 RNAimer] and contains the DNA sequence coding for a crRNA that binds complementary to Bla 2 target.  
 
This device contains loci coding for small RNAs that are able to target the catalytically-inactive, engineered [https://parts.igem.org/Part:BBa_K1150000 dCas9] protein to the Bla locus target 2 .  This RNaimer is bulid of the uniCAS [https://parts.igem.org/Part:BBa_K1150034 RNAimer] and contains the DNA sequence coding for a crRNA that binds complementary to Bla 2 target.  
  

Revision as of 23:16, 4 October 2013

uniCAS RNAimer to Bla Target 2


RNaimer (Bla target 2)
Function Targeting VEGF2 with Cas9
Use in Mammalian cells
RFC standard RFC 25
Backbone pSB1C3
Submitted by [http://2013.igem.org/Team:Freiburg Freiburg 2013]


Usage and Biology

This device contains loci coding for small RNAs that are able to target the catalytically-inactive, engineered dCas9 protein to the Bla locus target 2 . This RNaimer is bulid of the uniCAS RNAimer and contains the DNA sequence coding for a crRNA that binds complementary to Bla 2 target.


Freiburg 2013 used this plasmid to test the efficiency of target Bla2 by activating or repressing a SEAP reporter gene.