Difference between revisions of "Part:BBa K1150040"
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This device contains loci coding for small RNAs that are able to target the catalytically-inactive, engineered [https://parts.igem.org/Part:BBa_K1150000 dCas9] protein to the Bla locus target 2 . This RNaimer is bulid of the uniCAS [https://parts.igem.org/Part:BBa_K1150034 RNAimer] and contains the DNA sequence coding for a crRNA that binds complementary to Bla 2 target. | This device contains loci coding for small RNAs that are able to target the catalytically-inactive, engineered [https://parts.igem.org/Part:BBa_K1150000 dCas9] protein to the Bla locus target 2 . This RNaimer is bulid of the uniCAS [https://parts.igem.org/Part:BBa_K1150034 RNAimer] and contains the DNA sequence coding for a crRNA that binds complementary to Bla 2 target. | ||
Revision as of 23:16, 4 October 2013
uniCAS RNAimer to Bla Target 2
| RNaimer (Bla target 2) | |
|---|---|
| Function | Targeting VEGF2 with Cas9 |
| Use in | Mammalian cells |
| RFC standard | RFC 25 |
| Backbone | pSB1C3 |
| Submitted by | [http://2013.igem.org/Team:Freiburg Freiburg 2013] |
Usage and Biology
This device contains loci coding for small RNAs that are able to target the catalytically-inactive, engineered dCas9 protein to the Bla locus target 2 . This RNaimer is bulid of the uniCAS RNAimer and contains the DNA sequence coding for a crRNA that binds complementary to Bla 2 target.
Freiburg 2013 used this plasmid to test the efficiency of target Bla2 by activating or repressing a SEAP reporter gene.