Difference between revisions of "Part:BBa K1150040"

Revision as of 23:05, 4 October 2013

uniCAS RNAimer to Bla Target 2

RNaimer (Bla target 2)
Function	Targeting VEGF2 with Cas9
Use in	Mammalian cells
RFC standard	RFC 25
Backbone	pSB1C3
Submitted by	[http://2013.igem.org/Team:Freiburg Freiburg 2013]

Usage and Biology

This device contains loci coding for small RNAs that are able to target the catalytically-inactive, engineered dCas9 protein to the Bla locus target 2 . This RNaimer is bulid of the uniCAS RNAimer and contains the DNA sequence coding for a crRNA that binds complementary to Bla 2 target.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 224
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 216

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K1150040 short</partinfo>
-.
-<!-- Add more about the biology of this part here
+{| style="color:black" cellpadding="6" cellspacing="1" border="2" align="right"
+! colspan="2" style="background:#FFBF00;"|RNaimer (Bla target 2)
+|-
+|'''Function'''
+|Targeting VEGF2 with Cas9
+|-
+|'''Use in'''
+|Mammalian cells
+|-
+|'''RFC standard'''
+|[https://parts.igem.org/Help:Assembly_standard_25 RFC 25]
+|-
+|'''Backbone'''
+|pSB1C3<br>
+|-
+|'''Submitted by'''
+|[http://2013.igem.org/Team:Freiburg Freiburg 2013]
+|}
 ===Usage and Biology===
+This device contains loci coding for small RNAs that are able to target the catalytically-inactive, engineered [https://parts.igem.org/Part:BBa_K1150000 dCas9] protein to the Bla locus target 2 .  This RNaimer is bulid of the uniCAS [https://parts.igem.org/Part:BBa_K1150034 RNAimer] and contains the DNA sequence coding for a crRNA that binds complementary to Bla 2 target.
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