Difference between revisions of "Part:BBa K1123019"
(→‎Characterization)
 
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===Characterization===
 
===Characterization===
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For the iGEM 2013 Purdue team we characterized our parts according to their characterization datasheets. The data sheet for this particular biobrick can be found [[Media:Datasheet_BBa_K1123019.pdf | here]].
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====Characterization by the 2013 TU-Eindhoven iGEM team====
 
This part was created for use in the 2013 TU-Eindhoven iGEM project. Unfortunately however we did not get round to completing a proper characterization for this part as it went largely unused.  
 
This part was created for use in the 2013 TU-Eindhoven iGEM project. Unfortunately however we did not get round to completing a proper characterization for this part as it went largely unused.  
  

Latest revision as of 22:58, 4 October 2013

Poly(Arginine-Serine) Protein

This part contains the DNA sequence of a protein of our own design. We first repeated the Arginine-Serine amino acid pair 6 times to obtain a sequence of 12 amino acids. This sequence was then repeated a total of 36 times. Between these repeats no extra amino acids were added. The idea behind this protein was to provide ourselves with a high concentration of amino acids with amide groups which we could then use to provide CEST contrast in an MRI. The protein can be placed behind a promoter of your choice and expressed.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Characterization

For the iGEM 2013 Purdue team we characterized our parts according to their characterization datasheets. The data sheet for this particular biobrick can be found here.

Characterization by the 2013 TU-Eindhoven iGEM team

This part was created for use in the 2013 TU-Eindhoven iGEM project. Unfortunately however we did not get round to completing a proper characterization for this part as it went largely unused.