Difference between revisions of "Part:BBa K1041004"
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<partinfo>BBa_K1041004 short</partinfo>
 
<partinfo>BBa_K1041004 short</partinfo>
  
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Team NRP-UEA_Norwich 2013 desgined this part using biobrick BBa_K1041001. This biobrick contains an Nde1 site after its promoter sequence, enabling a restriction digest to be performed. The Neomycin resistance coding gene was excised from BBa_K1041001 and a gene encoding Gus ligated in front of the promoter of BBa_K1041001 to create a new biobrick.
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Team NRP-UEA_Norwich 2013 desgined this part using biobrick BBa_K1041001. This biobrick contains an Nde1 site after its promoter sequence, enabling a restriction digest to be performed. The Neomycin resistance coding gene would be excised from BBa_K1041001 and a gene encoding Gus ligated downstream of the promoter of BBa_K1041001 to create a new biobrick.
  
 
<!-- Add more about the biology of this part here
 
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Revision as of 22:56, 4 October 2013

AntG Promoter + Gus gene

Team NRP-UEA_Norwich 2013 desgined this part using biobrick BBa_K1041001. This biobrick contains an Nde1 site after its promoter sequence, enabling a restriction digest to be performed. The Neomycin resistance coding gene would be excised from BBa_K1041001 and a gene encoding Gus ligated downstream of the promoter of BBa_K1041001 to create a new biobrick.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 498
    Illegal NgoMIV site found at 630
    Illegal NgoMIV site found at 1227
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 896