Difference between revisions of "Part:BBa K1055000:Experience"
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As shown in Figure 1 and 4, the expression of mKate is not toxic towards the bacterial host ''E.coli'' Bl21(DE3). The growth rate was in a usual time scale of approximately 30 minutes. | As shown in Figure 1 and 4, the expression of mKate is not toxic towards the bacterial host ''E.coli'' Bl21(DE3). The growth rate was in a usual time scale of approximately 30 minutes. | ||
− | [[Image: | + | [[Image:Grow_log.png|400px|thumb|center|Figure 4. '''Semi logarithmic scaled growth curves of ''E.coli'' BL21(DE3) WT, BL21DE3 mKate and BL21DE3 LSSmOrange. The ln (OD600 values) are plotted against the time [min].''']] |
===User Reviews=== | ===User Reviews=== |
Revision as of 22:30, 4 October 2013
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how you used this part and how it worked out.
Applications of BBa_K1055000
Characterization mKate
mKate is a red fluorescent protein that we want to use as a acceptor for FRET (Figure 3). According to [http://www.evrogen.com/products/basicFPs.shtml Evrogen] mKate has an excitation maximum at 588 nm and an emission maximum at 633 nm. Figure 2 shows that the excitation maximum is at 579 nm and the emission maximum is 611 nm. The emission maximum is big enough to get stimulated by LSSmOrange, the other fluorescent protein. Of course, we checked also the emission and excitation of our BL21(DE3) cells (data not shown) and we can eliminate the theories that these cells disturb this florescence measurement.
As shown in Figure 1 and 4, the expression of mKate is not toxic towards the bacterial host E.coli Bl21(DE3). The growth rate was in a usual time scale of approximately 30 minutes.
User Reviews
UNIQ3c1158250cd646ce-partinfo-00000000-QINU UNIQ3c1158250cd646ce-partinfo-00000001-QINU