Difference between revisions of "Part:BBa K1055000:Experience"
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− | As shown in Figure 4 the expression of mKate is not toxic towards the bacterial host ''E.coli'' Bl21(DE3) | + | As shown in Figure 4 the expression of mKate is not toxic towards the bacterial host ''E.coli'' Bl21(DE3). |
[[Image:mKate_bac.png|400px|thumb|Figure 3. '''Bl21-DE3 cells with mKate ''']] | [[Image:mKate_bac.png|400px|thumb|Figure 3. '''Bl21-DE3 cells with mKate ''']] |
Revision as of 22:14, 4 October 2013
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Applications of BBa_K1055000
Characterization mKate
mKate is a red fluorescent protein that we want to use as a acceptor for FRET (Figure 1). According to [http://www.evrogen.com/products/basicFPs.shtml Evrogen] mKate has an excitation maximum at 588 nm and an emission maximum at 633 nm. Figure 2 shows that the excitation maximum is at 579 nm and the emission maximum is 611 nm. The emission maximum is big enough to get stimulated by LSSmOrange, the other fluorescent protein. Of course, we checked also the emission and excitation of our BL21(DE3) cells (data not shown) and we can eliminate the theories that these cells disturb this florescence measurement.
As shown in Figure 4 the expression of mKate is not toxic towards the bacterial host E.coli Bl21(DE3).
User Reviews
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