Difference between revisions of "Part:BBa K1055000:Experience"
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''Characterization mKate'' | ''Characterization mKate'' | ||
− | [[Image:MKate-LSSmOrange_FRET_Pair.png|400px|thumb|Figure 2. '''Overlay of excitation | + | [[Image:MKate-LSSmOrange_FRET_Pair.png|400px|thumb|Figure 2. '''Overlay of excitation spectrum (dashed line) and emission spectrum (solid line) of mKate and LSSmOrange ''']] |
− | mKate2 is a red fluorescent protein that we want to use as a acceptor for FRET. According to | + | mKate2 is a red fluorescent protein that we want to use as a acceptor for FRET. According to [http://www.evrogen.com/products/basicFPs.shtml Evrogen] mKate2 has an excitation maximum at 588 nm and an emission maximum at 633 nm. The emission maximum is big enough to get stimulated by [https://parts.igem.org/wiki/index.php?title=Part:BBa_K1055001 LSSmOrange], the other fluorescent protein. Of course, we checked also the emission and excitation of our BL21(DE3) cells (data not shown) and we can eliminate the theories that these cells disturb this florescence measurement. |
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Applications of BBa_K1055000
Characterization mKate
mKate2 is a red fluorescent protein that we want to use as a acceptor for FRET. According to [http://www.evrogen.com/products/basicFPs.shtml Evrogen] mKate2 has an excitation maximum at 588 nm and an emission maximum at 633 nm. The emission maximum is big enough to get stimulated by LSSmOrange, the other fluorescent protein. Of course, we checked also the emission and excitation of our BL21(DE3) cells (data not shown) and we can eliminate the theories that these cells disturb this florescence measurement.
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