Difference between revisions of "Part:BBa K1152007"

Revision as of 21:56, 4 October 2013

Helper construct for NRP-Indigoidine-tagging

This part serves as a template for the labeling of non-ribosomal peptides with indigoidine. The desired order of modules can be used to replace the suicide gen ccdB. Therefore, selection of positive transformed clones gets a lot easier and nascent non-ribosomal peptides are tagged by the Indigoidine residue attached during synthesis. Since the blue pigment Indigoidine can be seen without auxillary means, analytical procedures can be faciliated a lot. Instead of complex labeling, tagging or work up procedures, simple devices, like a TLC can be used to assess qualitativ differences.

To verify the genotype of our construct colony-PCRs, and restriction digests with Pst1 and EcoRI were conducted. Assembly overhang regions were sequenced as well.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 3061
Illegal BamHI site found at 891
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 341
Illegal SapI.rc site found at 4324

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 <partinfo>BBa_K1152007 short</partinfo>
-This part serves as a template for the labeling of non-ribosomal peptides with indigoidine. The desired order of modules can be used to replace the suicide gen ccdB. Therefore, selection of positive transformed clones gets a lot easier and nascent non-ribosomal peptides are tagged by the Indigoidine residue attached during synthesis. Since the blue pigment Indigoidine can be seen without auxillary means, analytical procedures can be faciliated a lot. Instead of complex labeling, tagging or work up procedures, simple devices, like a TLC can be used to asses qualitativ differences.
+This part serves as a template for the labeling of non-ribosomal peptides with indigoidine. The desired order of modules can be used to replace the suicide gen ccdB. Therefore, selection of positive transformed clones gets a lot easier and nascent non-ribosomal peptides are tagged by the Indigoidine residue attached during synthesis. Since the blue pigment Indigoidine can be seen without auxillary means, analytical procedures can be faciliated a lot. Instead of complex labeling, tagging or work up procedures, simple devices, like a TLC can be used to assess qualitativ differences.
+To verify the genotype of our construct colony-PCRs, and restriction digests with Pst1 and EcoRI were conducted. Assembly overhang regions were sequenced as well.
 <!-- Add more about the biology of this part here