Difference between revisions of "Part:BBa K1104401:Design"

 
 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K1104401 short</partinfo>
 
<partinfo>BBa_K1104401 short</partinfo>
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===Design Notes===
 
===Design Notes===
many design considerations taken into account
 
  
  
  
 
===Source===
 
===Source===
 
+
PCR from ''Zymomonas mobilis'' using the following primers:
z
+
* gcttctag atgagttatactgtcggtacctatttagcg
 +
* ggactgcagcggccgctactagta ctagaggagcttgttaacaggcttacg
  
 
===References===
 
===References===

Latest revision as of 21:43, 4 October 2013

pyruvate decarboxyase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1015
    Illegal AgeI site found at 1162
    Illegal AgeI site found at 1540
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 49
    Illegal SapI site found at 1126


Design Notes

Source

PCR from Zymomonas mobilis using the following primers:

  • gcttctag atgagttatactgtcggtacctatttagcg
  • ggactgcagcggccgctactagta ctagaggagcttgttaacaggcttacg

References