Difference between revisions of "Part:BBa K1152007"

 
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<partinfo>BBa_K1152007 short</partinfo>
 
<partinfo>BBa_K1152007 short</partinfo>
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===Usage and Biology===
 
===Usage and Biology===
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This part serves as a template for the labeling of non-ribosomal peptides with indigoidine. It consists of three fragments: ccdB, the C module of TycC2, indC, an Indigoidine synthetaseand the backbone. The desired order of modules can be used to replace the suicide gene ccdB. Therefore, selection of positive transformed clones gets a lot easier and nascent non-ribosomal peptides are tagged by the Indigoidine residue attached during synthesis. Since the blue pigment Indigoidine can be seen without auxillary means, analytical procedures can be faciliated a lot. Instead of complex labeling, tagging or work up procedures, simple devices, like a TLC can be used to asses qualitativ differences. As Indigoidine has some toxic effectsthis blue pigment could lead to slightly smaller colonies and can take some time after induction before colonies will turn blue (we experienced even more than two days).
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>

Revision as of 21:35, 4 October 2013

Helper construct for NRP-Indigoidine-tagging

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Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 3061
    Illegal BamHI site found at 891
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 341
    Illegal SapI.rc site found at 4324