Difference between revisions of "Part:BBa K1150007"

Revision as of 21:20, 4 October 2013

COP1

COP1
Function	interaction partner of UVR8,reacts to UVB light
Use in	Mammalians
RFC standard	RFC 25
Backbone	pSB1C3
Organism	Arabidopsis thaliana
Source	AG Weber, Freiburg
Submitted by	[http://2013.igem.org/Team:Freiburg Freiburg 2013]

Sunlight is essential for plants: Not only as an energy source but also as information for their growth cycle. Therefore they need mechanisms to detect different light wavelengths. E3-ubiquitin ligase CONSTITUTIVELY PHOTOMORPHOGENIC 1 (COP1) is the interaction partner of ultraviolet B (UVB) light receptor UVR8 [1]. The ubiqutin ligase activity of this COP1 is eliminated, only the WD40 domain remains. This minimizes the risk of cross talk in mammalian cells [2].

Biology and Usage

When UVB light is perceived by the UVR8 photoreceptor it monomerizes and therefore changes in its open confuguration. Then it recruits COP1. This is a signal for plant cells to react to UVB light, for example with photomorphogenesis [3]. Freiburg 2013 used this part to engineer a UVB light induced mechanism to activate gene expression. You need a combination of these BioBricks: Cas9-UVR8, COP1-VP16, RNaimer

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 787
Illegal BsaI.rc site found at 864
Illegal SapI.rc site found at 376

References

[1] Rizzini L., Favory J.-J., Cloix C., Faggionato D., O'Hara A., Kaiserli E., Baumeister R., Schäfer E., Nagy F., Jenkins G. I., Ulm R. (2011). Perception of UV-B by the Arabidopsis UVR8 protein. Science 332

[2] Müller K., Engesser R., Schulz S., Steinberg T., Tomakidi P., Weber C. C., Ulm R., Timmer J., Zurbriggen M. D., Weber W. (2013). Multi-chromatic control of mammalian gene expression and signaling. Nucleic Acids Research Vol. 41, No. 12

[3] Favory J.-J., Stec A., Gruber H., Rizzini L., Oravecz A., Funk M., Albert A., Cloix C., Jenkins G. I., Oakeley E. J., Seidlitz H. K., Nagy F., Ulm R. (2009). Interaction of COP1 and UVR8 regulates UV-B-induced photomorphogenesis and stress acclimation in Arabidopsis. The EMBO Journal 28, 591-600

@@ Line 34: / Line 34: @@
 ==Biology and Usage==
-When UVB light is perceived by the UVR8 photoreceptor ist monomerizes and therefore changes in its open confuguration. Then it recruits COP1. This is a signal for plant cells to react to UVB light, for example with photomorphogenesis [3].
+When UVB light is perceived by the UVR8 photoreceptor it monomerizes and therefore changes in its open confuguration. Then it recruits COP1. This is a signal for plant cells to react to UVB light, for example with photomorphogenesis [3].
 Freiburg 2013 used this part to engineer a UVB light induced mechanism to activate gene expression. You need a combination of these BioBricks: [https://parts.igem.org/Part:BBa_K1150029 Cas9-UVR8], [https://parts.igem.org/Part:BBa_K1150030 COP1-VP16], [https://parts.igem.org/Part:BBa_K1150034 RNaimer]