Difference between revisions of "Part:BBa K1150005"
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Illumination with red light (660 nm wavelength) leads to binding of Phytochrome Interaction Factor 6 (PIF6) to Phytochrome B. The interaction can be abolished by illumination with far-red light (740 nm) wavelength. | Illumination with red light (660 nm wavelength) leads to binding of Phytochrome Interaction Factor 6 (PIF6) to Phytochrome B. The interaction can be abolished by illumination with far-red light (740 nm) wavelength. | ||
| − | A framework using the unique properties of PhyB and PIF6 was designed to enable the recruitment of different effector proteins to any desired gene locus. The system was designed to work as described: Fusion proteins linking dCas9 to PIF6 were designed, as well as fusion proteins linking the interaction partner of PIF6 – PhyB – to several effector molecules such as VP16 or KRAB. | + | A framework using the unique properties of PhyB and PIF6 was designed to enable the recruitment of different effector proteins to any desired gene locus. The system was designed to work as described: Fusion proteins linking dCas9 to PIF6 were designed, as well as fusion proteins linking the interaction partner of PIF6 – PhyB – to several effector molecules such as [https://parts.igem.org/Part:BBa_K1150001 VP16] or [https://parts.igem.org/Part:BBa_K1150002 KRAB]. |
Revision as of 21:04, 4 October 2013
PIF6
| PIF6 | |
|---|---|
| Function | Phytochrome interaction factor |
| Use in | Mammalian cells |
| RFC standard | RFC 25 |
| Backbone | pSB1C3 |
| Organism | Arabidopsis thaliana |
| Source | AG Weber, Freiburg |
| Submitted by | [http://2013.igem.org/Team:Freiburg Freiburg 2013] |
The Phytochrome Interaction Factor 6 (PIF6) is derived from Arabidopsis thaliana.
Illumination with red light (660 nm wavelength) leads to binding of Phytochrome Interaction Factor 6 (PIF6) to Phytochrome B. The interaction can be abolished by illumination with far-red light (740 nm) wavelength.
A framework using the unique properties of PhyB and PIF6 was designed to enable the recruitment of different effector proteins to any desired gene locus. The system was designed to work as described: Fusion proteins linking dCas9 to PIF6 were designed, as well as fusion proteins linking the interaction partner of PIF6 – PhyB – to several effector molecules such as VP16 or KRAB.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]