Difference between revisions of "Part:BBa K1152003"

Revision as of 21:04, 4 October 2013

TycC6 Module from Tyrocidine

This is the coding sequence for the TycC6 module from a gene cluster of Brevibacillus parabrevis. TycC6 is the first module in this cluster encoding the non-ribosomal peptide synthetase of the cyclic decamer Tyrocidine. This specific module TycC6 is responsible for the binding of the last amino acid Leucine of the NRP. It consists of an A- (adenylation), a PCP- (peptide carrier protein) and an TE- (thioesterase) domain. After the additon of Leucine from TycC, the decapeptide undergoes cyclization to the final native Tyrocidine.

This biobrick can be used for the design of different NRPS. It was used in the synthetic Dipeptide, TripeptideI/II and TetrapeptideI/II of our [http://2013.igem.org/Team:Heidelberg/Project/Tyrocidine project].

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 48
Illegal SapI.rc site found at 1165
Illegal SapI.rc site found at 1402

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K1152003 short</partinfo>
-This is the CDS for TycC6 module from the Tyrocidine gene cluster of ''Brevibacillus Parabrevis''. It is the first module in this cluster for the Non-Ribosomal-Peptide-Synthetase of the cyclic decamer Tyrocidine. This specific module TycC6 is responsiple for the binding of the last amino acid Leucine of the NRP. It consists of the A- (adenylation), PCP- (peptide carrier protein) and the TE- (thioesterase) domain. After the additon of Leucine from TycC the decapeptide undergoes cyclization to the final native Tyrocidine.
-This bio brick can be used for the design of different NRPS. It was used in the synthetic Dipeptide, TripeptideI/II and TetrapeptideI/II of our project.
+This is the coding sequence for the TycC6 module from a gene cluster of ''Brevibacillus parabrevis''.
+TycC6 is the first module in this cluster encoding the non-ribosomal peptide synthetase of the cyclic decamer Tyrocidine.
+This specific module TycC6 is responsible for the binding of the last amino acid Leucine of the NRP. It consists of an A- (adenylation), a PCP- (peptide carrier protein) and an TE- (thioesterase) domain. After the additon of Leucine from TycC, the decapeptide undergoes cyclization to the final native Tyrocidine.
+This biobrick can be used for the design of different NRPS. It was used in the synthetic Dipeptide, TripeptideI/II and TetrapeptideI/II of our [http://2013.igem.org/Team:Heidelberg/Project/Tyrocidine project].
 <!-- Add more about the biology of this part here
 ===Usage and Biology===