Difference between revisions of "Part:BBa K1152000"

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This biobrick can be used for the design of different NRP synthetases.  
 
This biobrick can be used for the design of different NRP synthetases.  
It was used in the synthetic Tripeptide I of our project.
+
It was used in the synthetic Tripeptide I of our [http://2013.igem.org/Team:Heidelberg/Project/Tyrocidine project].
  
 
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Revision as of 21:01, 4 October 2013

TycA_deltaCom Module from Tyrocidine

This is the CDS for TycA_deltaCom module from the Tyrocidine gene cluster of Brevibacillus parabrevis. It is the first module in gene cluster encoding the non-ribosomal peptide (NRP) synthetase of the cyclic decamer Tyrocidine. This specific module TycA_deltaCom is responsiple for the binding of the first amino acid D-Phenyl of the NRP. It consists of the A- (adenylation), PCP- (peptide-carrier protein) and the E- (epimerization) domain. After tycA follows the tycB gene with three modules.

This biobrick can be used for the design of different NRP synthetases. It was used in the synthetic Tripeptide I of our [http://2013.igem.org/Team:Heidelberg/Project/Tyrocidine project].

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2518