Difference between revisions of "Part:BBa K1149008"
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Encodes a peptidase from proteinase K family, breaks down polylactic acid. See [https://parts.igem.org/Part:BBa_K1149007 here] for construct with secretion tag. | Encodes a peptidase from proteinase K family, breaks down polylactic acid. See [https://parts.igem.org/Part:BBa_K1149007 here] for construct with secretion tag. | ||
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<h2>References: </h2> | <h2>References: </h2> |
Latest revision as of 20:55, 4 October 2013
Proteinase K (extracellular)
Encodes a peptidase from proteinase K family, breaks down polylactic acid. See here for construct with secretion tag.
References:
Larsen AN, Moe E, Helland R, Gjellesvik DR, Willassen NP.Characterization of a recombinantly expressed proteinase K-like enzyme from a psychrotrophic Serratia sp. FEBS J. 2006 Jan;273(1):47-60.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 125
Illegal NheI site found at 1009 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 356
Illegal BamHI site found at 65 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 210
Illegal AgeI site found at 1531 - 1000COMPATIBLE WITH RFC[1000]