Difference between revisions of "Part:BBa K1152000"

Revision as of 20:33, 4 October 2013

TycA_deltaCom Module from Tyrocidine

This is the CDS for TycA_deltaCom module from the Tyrocidine gene cluster of Brevibacillus parabrevis. It is the first module in gene cluster encoding the non-ribosomal peptide (NRP) synthetase of the cyclic decamer Tyrocidine. This specific module TycA_deltaCom is responsiple for the binding of the first amino acid D-Phenyl of the NRP. It consists of the A- (adenylation), PCP- (peptide-carrier protein) and the E- (epimerization) domain. After tycA follows the tycB gene with three modules.

This biobrick can be used for the design of different NRP synthetases. It was used in the synthetic Tripeptide I of our project.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 2518

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K1152000 short</partinfo>
-This is the CDS for TycA_deltaCom module from the Tyrocidine gene cluster of Brevibacillus Parabrevis.
+This is the CDS for TycA_deltaCom module from the Tyrocidine gene cluster of ''Brevibacillus parabrevis''.
-It is the first module in this cluster for the NonRibosomalPeptide-Synthetase of the cyclic decamer Tyrocidine.
+It is the first module in gene cluster encoding the non-ribosomal peptide (NRP) synthetase of the cyclic decamer Tyrocidine.
-This specific module Tyca_deltaCom is responsiple for the binding of the first amino acid D-Phenyl of the NRP.
+This specific module TycA_deltaCom is responsiple for the binding of the first amino acid D-Phenyl of the NRP.
-It consists of the A- (adenylation), PCP- (peptide carrier protein) and the E- (epimerization) domain. After Tyc A follows the TycB gene with three modules.
+It consists of the A- (adenylation), PCP- (peptide-carrier protein) and the E- (epimerization) domain. After tycA follows the tycB gene with three modules.
-This bio brick can be used for the design of different NRPS.
+This biobrick can be used for the design of different NRP synthetases.
 It was used in the synthetic Tripeptide I of our project.