Difference between revisions of "Part:BBa K1045016:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | The inverse DarR sequence was cut out from [[Part:BBa_K1045001|BBa_K1045001]] and ligated into the plasmid carrying[[Part:BBa_K1045009|BBa_K1045009]] in a post-fixing composition. | + | The inverse DarR sequence was cut out from [[Part:BBa_K1045001|BBa_K1045001]] and ligated into the plasmid carrying [[Part:BBa_K1045009|BBa_K1045009]] in a post-fixing composition. |
===Source=== | ===Source=== | ||
Revision as of 19:54, 4 October 2013
Terminator BBa_B0015 with inversed Pre- and Suffix- DarR ORF with inversed Pre- and Suffix
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NotI site found at 718
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 731
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 168
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The inverse DarR sequence was cut out from BBa_K1045001 and ligated into the plasmid carrying BBa_K1045009 in a post-fixing composition.
Source
For this composite part, part BBa_K1045001 was used which sequence originated from PCR amplification from M. smegmatis chromosomal DNA. Part BBa_K1045009 originated from PCR amplification of plasmid DNA derived from the distribution kit 2013.
References
Lei Zhang, Weihui Li, and Zheng-Guo He (2013) “DarR, a TetR-like Transcriptional Factor, Is a Cyclic Di-AMP-responsive Repressor in Mycobacterium smegmatis”, THE JOURNAL OF BIOLOGICAL CHEMISTRY VOL. 288, NO. 5, pp. 3085–3096