Difference between revisions of "Part:BBa K1033111"

Latest revision as of 19:52, 4 October 2013

Zeaxanthin 7,8-cleavage dioxygenase (ZCD-His) with His tag

Cleaves zeaxanthin to begin the formation of saffron metabolites such as picrocrocin, crocin and safranal. This part is from a synthetic construct based on the gene from "Crocus Sativus". The protein expressed from this gene has been shown to form insoluable inclusion bodies in the cytoplasm of bacteria. These inclusion bodies form aggregates that inhibit cell growth. Therefore, it is recommended to increase th soluability of the enzyme, for example by fusing it with a histidine patch thioredoxin.

The his-tag (6xHis) was added to facilitate characterization of the construct.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 774
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K1033111 short</partinfo>
-Cleaves zeaxanthin to begin the formation of saffron metabolites such as picrocrocin, crocin and safranal. The his-tag (6xHis) was added to facilitate characterization of the construct.
+Cleaves zeaxanthin to begin the formation of saffron metabolites such as picrocrocin, crocin and safranal. This part is from a synthetic construct based on the gene from "Crocus Sativus". The protein expressed from this gene has been shown to form insoluable inclusion bodies in the cytoplasm of bacteria. These inclusion bodies form aggregates that inhibit cell growth. Therefore, it is recommended to increase th soluability of the enzyme, for example by fusing it with a histidine patch thioredoxin.
+The his-tag (6xHis) was added to facilitate characterization of the construct.
 <!-- Add more about the biology of this part here