Difference between revisions of "Part:BBa K1122676:Design"
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Latest revision as of 19:38, 4 October 2013
IPTG inducible pdc and adhB
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1109
Illegal AgeI site found at 2315 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
Presence of LacZ alpha peptide upstream of the ORFs coding for the enzymes enables blue- white screening.
Source
Z. mobilis gDNA, codon optimised for E. coli
References
INGRAM, L. O., CONWAY, T., CLARK, D. P., SEWELL, G. W. & PRESTON, J. F. 1987. GENETIC-ENGINEERING OF ETHANOL-PRODUCTION IN ESCHERICHIA-COLI. Applied and Environmental Microbiology, 53, 2420-2425.